Construct: ORF TRCN0000487915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020994.1_s317c1
- DNA Barcode:
- TAAGTGGTTAGCTTAGGCCAATGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CASR (846)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487915
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 846 | CASR | calcium sensing receptor | NM_000388.4 | 99.9% | 99.8% | 2244G>C;2968A>G;3031G>C |
| 2 | human | 846 | CASR | calcium sensing receptor | XM_006713789.3 | 99.9% | 99.8% | 2244G>C;2968A>G;3031G>C |
| 3 | human | 846 | CASR | calcium sensing receptor | XM_017007324.1 | 99.9% | 99.8% | 2244G>C;2968A>G;3031G>C |
| 4 | human | 846 | CASR | calcium sensing receptor | XM_017007325.1 | 99.9% | 99.8% | 2244G>C;2968A>G;3031G>C |
| 5 | human | 846 | CASR | calcium sensing receptor | NM_001178065.2 | 98.9% | 98.8% | (many diffs) |
| 6 | human | 846 | CASR | calcium sensing receptor | XM_005247837.2 | 84.8% | 84.6% | (many diffs) |
| 7 | mouse | 12374 | Casr | calcium-sensing receptor | NM_013803.3 | 87.5% | 93.6% | (many diffs) |
| 8 | mouse | 12374 | Casr | calcium-sensing receptor | XM_006521729.2 | 87.5% | 93.6% | (many diffs) |
| 9 | mouse | 12374 | Casr | calcium-sensing receptor | XM_006521730.2 | 87.5% | 93.6% | (many diffs) |
| 10 | mouse | 12374 | Casr | calcium-sensing receptor | XM_006521731.3 | 87.5% | 93.6% | (many diffs) |
| 11 | mouse | 12374 | Casr | calcium-sensing receptor | XM_006521732.2 | 87.5% | 93.6% | (many diffs) |
| 12 | mouse | 12374 | Casr | calcium-sensing receptor | XM_006521735.2 | 75.2% | 79.2% | (many diffs) |
| 13 | mouse | 12374 | Casr | calcium-sensing receptor | XM_017316856.1 | 72% | 76.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3306
- ORF length:
- 3234
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcattt tatagctgct gctgggtcct cttggcactc acctggcaca 121 cctctgccta cgggccagac cagcgagccc aaaagaaggg ggacattatc cttggggggc 181 tctttcctat tcattttgga gtagcagcta aagatcaaga tctcaaatca aggccggagt 241 ctgtggaatg tatcaggtat aatttccgtg ggtttcgctg gttacaggct atgatatttg 301 ccatagagga gataaacagc agcccagccc ttcttcccaa cttgacgctg ggatacagga 361 tatttgacac ttgcaacacc gtttctaagg ccttggaagc caccctgagt tttgttgctc 421 aaaacaaaat tgattctttg aaccttgatg agttctgcaa ctgctcagag cacattccct 481 ctacgattgc tgtggtggga gcaactggct caggcgtctc cacggcagtg gcaaatctgc 541 tggggctctt ctacattccc caggtcagtt atgcctcctc cagcagactc ctcagcaaca 601 agaatcaatt caagtctttc ctccgaacca tccccaatga tgagcaccag gccactgcca 661 tggcagacat catcgagtat ttccgctgga actgggtggg cacaattgca gctgatgacg 721 actatgggcg gccggggatt gagaaattcc gagaggaagc tgaggaaagg gatatctgca 781 tcgacttcag tgaactcatc tcccagtact ctgatgagga agagatccag catgtggtag 841 aggtgattca aaattccacg gccaaagtca tcgtggtttt ctccagtggc ccagatcttg 901 agcccctcat caaggagatt gtccggcgca atatcacggg caagatctgg ctggccagcg 961 aggcctgggc cagctcctcc ctgatcgcca tgcctcagta cttccacgtg gttggcggca 1021 ccattggatt cgctctgaag gctgggcaga tcccaggctt ccgggaattc ctgaagaagg 1081 tccatcccag gaagtctgtc cacaatggtt ttgccaagga gttttgggaa gaaacattta 1141 actgccacct ccaagaaggt gcaaaaggac ctttacctgt ggacaccttt ctgagaggtc 1201 acgaagaaag tggcgacagg tttagcaaca gctcgacagc cttccgaccc ctctgtacag 1261 gggatgagaa catcagcagt gtcgagaccc cttacataga ttacacgcat ttacggatat 1321 cctacaatgt gtacttagca gtctactcca ttgcccacgc cttgcaagat atatatacct 1381 gcttacctgg gagagggctc ttcaccaatg gctcctgtgc agacatcaag aaagttgagg 1441 cgtggcaggt cctgaagcac ctacggcatc taaactttac aaacaatatg ggggagcagg 1501 tgacctttga tgagtgtggt gacctggtgg ggaactattc catcatcaac tggcacctct 1561 ccccagagga tggctccatc gtgtttaagg aagtcgggta ttacaacgtc tatgccaaga 1621 agggagaaag actcttcatc aacgaggaga aaatcctgtg gagtgggttc tccagggagg 1681 tgcccttctc caactgcagc cgagactgcc tggcagggac caggaaaggg atcattgagg 1741 gggagcccac ctgctgcttt gagtgtgtgg agtgtcctga tggggagtat agtgatgaga 1801 cagatgccag tgcctgtaac aagtgcccag atgacttctg gtccaatgag aaccacacct 1861 cctgcattgc caaggagatc gagtttctgt cgtggacgga gccctttggg atcgcactca 1921 ccctctttgc cgtgctgggc attttcctga cagcctttgt gctgggtgtg tttatcaagt 1981 tccgcaacac acccattgtc aaggccacca accgagagct ctcctacctc ctcctcttct 2041 ccctgctctg ctgcttctcc agctccctgt tcttcatcgg ggagccccag gactggacgt 2101 gccgcctgcg ccagccggcc tttggcatca gcttcgtgct ctgcatctca tgcatcctgg 2161 tgaaaaccaa ccgtgtcctc ctggtgtttg aggccaagat ccccaccagc ttccaccgca 2221 agtggtgggg gctcaacctg cagttcctgc tggttttcct ctgcaccttc atgcagattg 2281 tcatctgtgt gatctggctc tacaccgcgc ccccctcaag ctaccgcaac caggagctgg 2341 aggatgagat catcttcatc acgtgccacg agggctccct catggccctg ggcttcctga 2401 tcggctacac ctgcctgctg gctgccatct gcttcttctt tgccttcaag tcccggaagc 2461 tgccggagaa cttcaatgaa gccaagttca tcaccttcag catgctcatc ttcttcatcg 2521 tctggatctc cttcattcca gcctatgcca gcacctatgg caagtttgtc tctgccgtag 2581 aggtgattgc catcctggca gccagctttg gcttgctggc gtgcatcttc ttcaacaaga 2641 tctacatcat tctcttcaag ccatcccgca acaccatcga ggaggtgcgt tgcagcaccg 2701 cagctcacgc tttcaaggtg gctgcccggg ccacgctgcg ccgcagcaac gtctcccgca 2761 agcggtccag cagccttgga ggctccacgg gatccacccc ctcctcctcc atcagcagca 2821 agagcaacag cgaagaccca ttcccacagc ccgagaggca gaagcagcag cagccgctgg 2881 ccctaaccca gcaagagcag cagcagcagc ccctgaccct cccacagcag caacgatctc 2941 agcagcagcc cagatgcaag cagaaggtca tctttggcag cggcacggtc accttctcac 3001 tgagctttga tgagccTCAG AAGAACGCCA TGGCCCACGG GAATTCTACG CACCAGAACT 3061 CCCTGGAGGC CCAGAAAAGC AGCGATACGC TGACCCGACA CCAGCCATTA CTCCCGCTGC 3121 AGTGCGGGGA AACGGACTTA GATCTGACCG TCCAGGAAAC AGGTCTGCAA GGACCTGTGG 3181 GTGGAGACCA GCGGCCAGAG GTGGAGGACC CTGAAGAGTT GTCCCCAGCA CTTGTAGTGT 3241 CCAGTTCACA GAGCTTTGTC ATCAGTGGTG GAGGCAGCAC TGTTACAGAA AACGTAGTGA 3301 ATTCATAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3361 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3421 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATAAGTG GTTAGCTTAG GCCAATGGAC 3481 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt