Construct: ORF TRCN0000488970
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020657.1_s317c1
- DNA Barcode:
- TGCTAGTCTATTTTCAGTACCAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SNRK (54861)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488970
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54861 | SNRK | SNF related kinase | NM_001100594.1 | 99.7% | 100% | 2295_2296insTGATC |
| 2 | human | 54861 | SNRK | SNF related kinase | NM_017719.5 | 99.7% | 100% | 2295_2296insTGATC |
| 3 | human | 54861 | SNRK | SNF related kinase | XM_005265245.3 | 99.7% | 100% | 2295_2296insTGATC |
| 4 | human | 54861 | SNRK | SNF related kinase | NM_001330750.1 | 72.9% | 73% | 0_1ins618;1677_1678insTGATC |
| 5 | mouse | 20623 | Snrk | SNF related kinase | NM_001164572.1 | 82.3% | 88.9% | (many diffs) |
| 6 | mouse | 20623 | Snrk | SNF related kinase | NM_133741.2 | 82.3% | 88.9% | (many diffs) |
| 7 | mouse | 20623 | Snrk | SNF related kinase | XM_006511998.3 | 82.3% | 88.9% | (many diffs) |
| 8 | mouse | 20623 | Snrk | SNF related kinase | XM_006511999.3 | 82.3% | 88.9% | (many diffs) |
| 9 | mouse | 20623 | Snrk | SNF related kinase | XM_006512000.3 | 82.3% | 88.9% | (many diffs) |
| 10 | mouse | 20623 | Snrk | SNF related kinase | XM_006512002.3 | 82.3% | 88.9% | (many diffs) |
| 11 | mouse | 20623 | Snrk | SNF related kinase | XM_006512003.3 | 82.3% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 102
- ORF end:
- 2397
- ORF length:
- 2295
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcag 61 gctccgcggc cgcccccttc accgaattct gttggaccag catggcagga tttaagcgag 121 ggtatgatgg aaagattgct ggattatatg atctggataa aaccttgggt cgaggccatt 181 ttgccgtggt taaacttgcc aggcatgtct ttacgggtga aaaggtggca gtaaaagtta 241 ttgacaagac aaaactggac actctagcta ctggtcatct tttccaggaa gtgagatgca 301 tgaaactagt gcagcatcct aacatcgtcc gcctttatga agttattgac acccagacca 361 aactatatct tattctagaa cttggggatg gaggagatat gtttgattat ataatgaaac 421 atgaggaggg tcttaatgaa gacttggcca agaagtattt tgctcagata gttcatgcta 481 tatcttattg ccataaactc catgtggttc acagagactt aaaaccagag aatgtagtct 541 tctttgaaaa acaaggtctt gtaaagttga cagactttgg gttcagcaac aaatttcaac 601 cagggaagaa gctcactaca agctgtggat ctcttgcata ttccgctcca gaaattctgc 661 ttggtgatga gtatgatgca cctgcagtag atatttggag tctgggagtg atccttttca 721 tgttggtgtg tgggcagccg ccctttcaag aagccaatga cagtgaaaca ctgacaatga 781 tcatggattg caaatataca gtaccatccc atgtgtctaa agagtgtaaa gacctaatca 841 cacggatgct acagagagat cccaagagaa gggcttcttt agaagagatt gaaaatcatc 901 cttggcttca gggagtggac ccttcaccag ctacaaagta taacattccc cttgtgtcat 961 acaaaaatct ctcggaagag gagcacaaca gcatcattca gcgcatggtg cttggggaca 1021 tagcggatcg agacgccatt gtagaagccc tggaaaccaa caggtataac catatcacag 1081 ccacatactt cctgctggct gaaaggatcc tgagagaaaa gcaagagaaa gaaatacaga 1141 ccagatctgc aagcccgagc aatatcaagg cccagtttag gcagtcatgg ccaaccaaaa 1201 ttgatgtacc ccaggacctt gaggatgacc tcacggccac tcctttgtcc cacgcgactg 1261 tccctcagtc tcctgctcgg gctgctgaca gtgtcctcaa tggccacagg agcaaaggcc 1321 tgtgtgactc agctaagaaa gatgacctcc ctgagttggc tggaccagca ctctctacgg 1381 tgccacccgc aagcttaaaa cccacagcca gtgggcggaa gtgtctgttc agggtggaag 1441 aagatgaaga ggaagatgag gaggacaaga aacccatgtc cctctcaaca caagtggttt 1501 tgcgccggaa gccatctgta accaaccgcc tgacatccag gaagagtgcg cccgtcctca 1561 accagatctt tgaggaaggg gaatctgacg atgagtttga catggatgag aatctgcctc 1621 ccaagttgag caggttaaag atgaatatag cttctccagg tacagttcac aaacgctacc 1681 accggaggaa aagtcagggc cggggctcca gctgcagtag ttcggagacc agtgatgatg 1741 attctgaaag ccggcggcgg ctcgataaag atagcgggtt cacctactcc tggcaccgac 1801 gggatagcag cgaggggccc cctggcagtg agggggatgg cgggggccag agcaagccaa 1861 gcaatgccag tggaggggtg gacaaggcca gccccagtga gaacaatgct ggtgggggca 1921 gtccctccag cggctcgggt ggcaacccca ccaatacatc gggtaccaca cgccgctgtg 1981 ccggccccag caactccatg cagctggcct ctcgcagtgc tggggagctc gttgagagcc 2041 tcaaactcat gagcctctgc cTCGGCTCCC AGCTTCATGG GAGCACCAAG TACATTATTG 2101 ATCCACAGAA TGGCTTGTCA TTTTCCAGTG TGAAAGTCCA AGAGAAATCT ACGTGGAAAA 2161 TGTGCATTAG CTCCACAGGG AATGCAGGGC AGGTCCCTGC AGTGGGCGGC ATAAAGTTTT 2221 TCTCTGACCA CATGGCAGAT ACCACCACTG AATTGGAACG GATAAAGAGC AAGAACCTGA 2281 AAAATAACGT GCTGCAGCTA CCTCTGTGCG AAAAGACCAT CTCTGTGAAC ATCCAGCGGA 2341 ACCCTAAGGA GGGGCTGCTG TGCGCATCCA GCCCAGCCAG CTGTTGCCAT GTCATCTGAT 2401 CTAGAAAGGG TGGGCGCGCC GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2461 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGC CCGTAACTTG 2521 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGCT AGTCTATTTT 2581 CAGTACCAGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt