Construct: ORF TRCN0000489052
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022025.1_s317c1
- DNA Barcode:
- ACCCTCTTGCATGCCTGCACGGGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RIC3 (79608)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489052
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_024557.6 | 100% | 100% | |
| 2 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_006718318.4 | 100% | 100% | |
| 3 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001206671.4 | 99.7% | 99.7% | 520_522delAGC |
| 4 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_006718317.4 | 92.6% | 92.6% | 520_606del |
| 5 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_017018287.2 | 92.6% | 92.6% | 520_606del |
| 6 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001346692.2 | 86.6% | 86.6% | 0_1ins147 |
| 7 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_024448684.1 | 86.6% | 86.6% | 0_1ins147 |
| 8 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001346694.2 | 86.4% | 86.4% | 0_1ins147;373_375delAGC |
| 9 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001346693.2 | 80.3% | 80.3% | 0_1ins147;373_459del |
| 10 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001206672.4 | 78.2% | 77.9% | 427_428ins240 |
| 11 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930900.3 | 63.5% | 1_32del;552_638del;1224_1738del | |
| 12 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001346690.2 | 54.3% | 53.8% | (many diffs) |
| 13 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001135109.4 | 50.8% | 50.8% | 120_121ins543 |
| 14 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_006718321.4 | 50.7% | 46.1% | (many diffs) |
| 15 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_024448685.1 | 48.6% | 47.8% | (many diffs) |
| 16 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_024448686.1 | 48.6% | 47.8% | (many diffs) |
| 17 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001346691.2 | 47.7% | 47.2% | (many diffs) |
| 18 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_001747957.2 | 43.6% | 1_32del;1137_2529del | |
| 19 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_428851.4 | 42.2% | 1_32del;552_638del;1224_2616del | |
| 20 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NR_144485.2 | 41.7% | 1_36del;158_206del;1190_2645del | |
| 21 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930896.3 | 41.2% | 1_32del;552_638del;1224_2679del | |
| 22 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_002957193.1 | 36.3% | 1_32del;1137_3036del | |
| 23 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930897.3 | 35.3% | 1_32del;552_638del;1224_3123del | |
| 24 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930898.3 | 35.1% | 1_32del;552_638del;1224_3141del | |
| 25 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_002957192.1 | 29% | 1_32del;1137_3801del | |
| 26 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930901.3 | 29% | 1_32del;552_554delAGC;1140_3804del | |
| 27 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930903.3 | 28.5% | (many diffs) | |
| 28 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_428848.4 | 28.3% | 1_32del;552_638del;1224_3888del | |
| 29 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_002957194.1 | 27.9% | (many diffs) | |
| 30 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_002957195.1 | 25.2% | 1_32del;551_552ins146;991_3655del | |
| 31 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930904.3 | 19.9% | (many diffs) | |
| 32 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_001747959.2 | 19.9% | (many diffs) | |
| 33 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930905.3 | 19.6% | (many diffs) | |
| 34 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NR_144484.2 | 17.4% | 1_36del;463_464ins94;1047_5691del | |
| 35 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NR_144497.2 | 12.2% | 1_36del;158_159ins397;744_5388del | |
| 36 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | NM_001311161.1 | 87.2% | 86.4% | (many diffs) |
| 37 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | NM_001038624.1 | 87% | 86.1% | (many diffs) |
| 38 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | XM_017322302.1 | 55.1% | 55.9% | (many diffs) |
| 39 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | NM_178780.3 | 54.9% | 55.8% | (many diffs) |
| 40 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | XM_006507942.2 | 49% | 46.8% | (many diffs) |
| 41 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | XM_006507943.3 | 49% | 46.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1173
- ORF length:
- 1104
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgtactcc acagtgcaga gagtcgctct ggcttctggg cttgtcctgg 121 ctctgtcgct gctgctgccc aaggccttcc tgtcccgcgg gaagcggcag gagccgccgc 181 cgacacctga aggaaaattg ggccgatttc cacctatgat gcatcatcac caggcaccct 241 cagatggcca gactcctggg gctcgtttcc agaggtctca ccttgccgag gcatttgcaa 301 aggccaaagg atcaggtgga ggtgctggag gaggaggtag tggaagaggt ctgatggggc 361 agattattcc aatctacggt tttgggattt ttttatatat actgtacatt ctatttaagc 421 tctcaaaggg gaaaacaact gcagaggatg ggaaatgcta tactgccatg cctggaaaca 481 cccacaggaa aattaccagt tttgagcttg ctcaactgca agaaaaactg aaggagacag 541 aagcagccat ggaaaaatta atcaacagag tgggacctaa tggtgagaga gcacagactg 601 tgacttctga ccaagagaaa cggttgctac atcagctccg agaaatcacc agggtcatga 661 aagaaggaaa attcattgac agattttctc cagagaaaga agctgaggag gccccttaca 721 tggaggactg ggaaggttac cctgaagaga cttacccaat ttatgacctt tcagactgta 781 tcaagcgtag gcaagaaaca atcttggtgg attacccTGA CCCAAAAGAA CTTTCTGCTG 841 AAGAAATAGC TGAAAGAATG GGAATGATAG AAGAGGAAGA ATCAGATCAT TTGGGTTGGG 901 AAAGTCTGCC CACTGACCCC AGAGCCCAGG AAGATAATTC TGTTACCTCG TGTGATCCAA 961 AGCCAGAAAC ATGTTCCTGC TGTTTTCATG AAGACGAGGA TCCTGCTGTC TTGGCAGAGA 1021 ATGCTGGATT CAGTGCAGAT AGCTACCCTG AGCAAGAGGA AACCACCAAA GAAGAGTGGT 1081 CCCAAGACTT TAAAGATGAA GGGTTGGGCA TCAGCACCGA TAAAGCATAT ACAGGCAGCA 1141 TGCTGAGGAA GCGTAACCCC CAGGGTTTAG AGTGAGACCC AGCTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 AACCCTCTTG CATGCCTGCA CGGGGACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt