Construct: ORF TRCN0000489162

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020382.1_s317c1
DNA Barcode:
ACTTTTCATACCCTAGATACCCAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CAMK2A (815)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489162
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 815 CAMK2A calcium/calmodulin dependen... NM_001363990.1 99.9% 100% 1377T>C
2 human 815 CAMK2A calcium/calmodulin dependen... NM_171825.2 99.9% 100% 1377T>C
3 human 815 CAMK2A calcium/calmodulin dependen... NM_001369025.2 99.8% 99.7% 1377T>C;1434G>C
4 human 815 CAMK2A calcium/calmodulin dependen... NM_001363989.1 97.6% 97.7% 984_1016del;1410T>C
5 human 815 CAMK2A calcium/calmodulin dependen... NM_015981.4 97.6% 97.7% 984_1016del;1410T>C
6 human 816 CAMK2B calcium/calmodulin dependen... NM_172083.3 80% 85.2% (many diffs)
7 human 816 CAMK2B calcium/calmodulin dependen... XM_005249864.3 77.8% 82.6% (many diffs)
8 human 816 CAMK2B calcium/calmodulin dependen... XM_017012665.1 77.7% 82.6% (many diffs)
9 human 816 CAMK2B calcium/calmodulin dependen... NM_172081.3 76.3% 81.1% (many diffs)
10 human 816 CAMK2B calcium/calmodulin dependen... XM_017012664.1 76.2% 81.1% (many diffs)
11 human 816 CAMK2B calcium/calmodulin dependen... NM_172084.3 75.5% 80.6% (many diffs)
12 human 816 CAMK2B calcium/calmodulin dependen... XM_006715784.2 74.4% 78.9% (many diffs)
13 human 816 CAMK2B calcium/calmodulin dependen... NM_172079.2 74.3% 78.8% (many diffs)
14 human 816 CAMK2B calcium/calmodulin dependen... NM_172080.3 74.2% 78.8% (many diffs)
15 human 816 CAMK2B calcium/calmodulin dependen... XM_005249862.3 73% 77.6% (many diffs)
16 human 816 CAMK2B calcium/calmodulin dependen... NM_001293170.2 71.2% 75.5% (many diffs)
17 human 816 CAMK2B calcium/calmodulin dependen... NM_172078.3 71.2% 75.5% (many diffs)
18 mouse 12322 Camk2a calcium/calmodulin-dependen... NM_177407.4 93% 99.7% (many diffs)
19 mouse 12322 Camk2a calcium/calmodulin-dependen... XM_017317805.2 93% 99.5% (many diffs)
20 mouse 12322 Camk2a calcium/calmodulin-dependen... XM_006525541.4 91% 97.5% (many diffs)
21 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514479.2 76.3% 81.3% (many diffs)
22 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514476.1 74.2% 78.9% (many diffs)
23 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514477.1 74.1% 78.9% (many diffs)
24 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514475.1 73.1% 77.8% (many diffs)
25 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314240.1 73% 77.6% (many diffs)
26 mouse 12323 Camk2b calcium/calmodulin-dependen... NM_001174054.1 72.6% 77.3% (many diffs)
27 mouse 12323 Camk2b calcium/calmodulin-dependen... NM_007595.5 71.1% 75.6% (many diffs)
28 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314239.1 71% 75.5% (many diffs)
29 mouse 12323 Camk2b calcium/calmodulin-dependen... NM_001174053.1 70.4% 74.9% (many diffs)
30 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514469.2 61.5% 65.2% (many diffs)
31 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514470.3 61.4% 65.2% (many diffs)
32 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514467.2 60% 63.7% (many diffs)
33 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314238.1 60% 63.7% (many diffs)
34 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314237.1 59.3% 62.9% (many diffs)
35 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514465.2 58.1% 61.6% (many diffs)
36 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514464.2 58% 61.5% (many diffs)
37 mouse 12322 Camk2a calcium/calmodulin-dependen... NM_001286809.1 35.3% 34.7% (many diffs)
38 mouse 12322 Camk2a calcium/calmodulin-dependen... NM_009792.3 34.5% 33.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1506
ORF length:
1434
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggccacc atcacctgca cccgcttcac ggaagagtac cagctcttcg
121aggaattggg caagggagcc ttctcggtgg tgcgaaggtg tgtgaaggtg ctggctggcc
181aggagtatgc tgccaagatc atcaacacaa agaagctgtc agccagagac catcagaagc
241tggagcgtga agcccgcatc tgccgcctgc tgaagcaccc caacatcgtc cgactacatg
301acagcatctc agaggaggga caccactacc tgatcttcga cctggtcact ggtggggaac
361tgtttgaaga tatcgtggcc cgggagtatt acagtgaggc ggatgccagt cactgtatcc
421agcagatcct ggaggctgtg ctgcactgcc accagatggg ggtggtgcac cgggacctga
481agcctgagaa tctgttgctg gcctccaagc tcaagggtgc cgcagtgaag ctggcagact
541ttggcctggc catagaggtg gagggggagc agcaggcatg gtttgggttt gcagggactc
601ctggatatct ctccccagaa gtgctgcgga aggacccgta cgggaagcct gtggacctgt
661gggcttgtgg ggtcatcctg tacatcctgc tggttgggta ccccccgttc tgggatgagg
721accagcaccg cctgtaccag cagatcaaag ccggcgccta tgatttccca tcgccggaat
781gggacactgt caccccggaa gccaaggatc tgatcaataa gatgctgacc attaacccat
841ccaaacgcat cacagctgcc gaagccctta agcacccctg gatctcgcac cgctccaccg
901tggcatcctg catgcacaga caggagaccg tggactgcct gaagaagttc aatgccagga
961ggaaactgaa gggagccatt ctcaccacga tgctggccac caggaacttc tccggaggga
1021agagtggggg aaacaagaag agcgatggtg tgaaggaatc ctcagagagc accaacacca
1081ccatcgagga tgaagacacc aaagtgcgga aacaggaaat tataaaagtg acagagcagc
1141tgattgaagc cataagcaat ggagattttg agtcctacac gaagatgtgc gaccctggca
1201tgacagccTT CGAACCTGAG GCCCTGGGGA ACCTGGTTGA GGGCCTGGAC TTCCATCGAT
1261TCTATTTTGA AAACCTGTGG TCCCGGAACA GCAAGCCCGT GCACACCACC ATCCTGAATC
1321CCCACATCCA CCTGATGGGC GACGAGTCAG CCTGCATCGC CTACATCCGC ATCACGCAGT
1381ACCTGGACGC TGGCGGCATC CCACGCACCG CCCAGTCGGA GGAGACCCGT GTCTGGCACC
1441GCCGGGACGG CAAATGGCAG ATCGTCCACT TCCACAGATC TGGGGCGCCC TCCGTCCTGC
1501CCCACTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1561CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1621TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACTTTT CATACCCTAG ATACCCACAC
1681GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)