Construct: ORF TRCN0000489669
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020309.1_s317c1
- DNA Barcode:
- GCGGGCGGAAGTCATGTGCGCTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- AKT3 (10000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489669
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_001370074.1 | 100% | 100% | |
| 2 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_005465.7 | 100% | 100% | |
| 3 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_001206729.1 | 95.8% | 94.3% | (many diffs) |
| 4 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_181690.2 | 95.8% | 94.3% | (many diffs) |
| 5 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024446000.1 | 89.4% | 88.9% | (many diffs) |
| 6 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_016999985.1 | 87.2% | 87.2% | 0_1ins183 |
| 7 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024446892.1 | 60.4% | 58% | (many diffs) |
| 8 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024447938.1 | 50.5% | 49.2% | (many diffs) |
| 9 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_011544014.2 | 50.2% | 42.1% | (many diffs) |
| 10 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | NM_011785.3 | 94% | 99.5% | (many diffs) |
| 11 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496818.3 | 94% | 99.5% | (many diffs) |
| 12 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496819.3 | 94% | 99.5% | (many diffs) |
| 13 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_011238805.2 | 84.4% | 85.6% | (many diffs) |
| 14 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_017320418.1 | 81.6% | 86.8% | (many diffs) |
| 15 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496821.3 | 54.1% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 1521
- ORF length:
- 1437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgagcg atgttaccat tgtgaaagaa ggttgggttc 121 agaagagggg agaatatata aaaaactgga ggccaagata cttccttttg aagacagatg 181 gctcattcat aggatataaa gagaaacctc aagatgtgga tttaccttat cccctcaaca 241 acttttcagt ggcaaaatgc cagttaatga aaacagaacg accaaagcca aacacattta 301 taatcagatg tctccagtgg actactgtta tagagagaac atttcatgta gatactccag 361 aggaaaggga agaatggaca gaagctatcc aggctgtagc agacagactg cagaggcaag 421 aagaggagag aatgaattgt agtccaactt cacaaattga taatatagga gaggaagaga 481 tggatgcctc tacaacccat cataaaagaa agacaatgaa tgattttgac tatttgaaac 541 tactaggtaa aggcactttt gggaaagtta ttttggttcg agagaaggca agtggaaaat 601 actatgctat gaagattctg aagaaagaag tcattattgc aaaggatgaa gtggcacaca 661 ctctaactga aagcagagta ttaaagaaca ctagacatcc ctttttaaca tccttgaaat 721 attccttcca gacaaaagac cgtttgtgtt ttgtgatgga atatgttaat gggggcgagc 781 tgtttttcca tttgtcgaga gagcgggtgt tctctgagga ccgcacacgt ttctatggtg 841 cagaaattgt ctctgccttg gactatctac attccggaaa gattgtgtac cgtgatctca 901 agttggagaa tctaatgctg gacaaagatg gccacataaa aattacagat tttggacttt 961 gcaaagaagg gatcacagat gcagccacca tgaagacatt ctgtggcact ccagaatatc 1021 tggcaccaga ggtgttagaa gataatgact atggccgagc agtagactgg tggggcctag 1081 gggttgtcat gtatgaaatg atgtgtggga ggttaCCTTT CTACAACCAG GACCATGAGA 1141 AACTTTTTGA ATTAATATTA ATGGAAGACA TTAAATTTCC TCGAACACTC TCTTCAGATG 1201 CAAAATCATT GCTTTCAGGG CTCTTGATAA AGGATCCAAA TAAACGCCTT GGTGGAGGAC 1261 CAGATGATGC AAAAGAAATT ATGAGACACA GTTTCTTCTC TGGAGTAAAC TGGCAAGATG 1321 TATATGATAA AAAGCTTGTA CCTCCTTTTA AACCTCAAGT AACATCTGAG ACAGATACTA 1381 GATATTTTGA TGAAGAATTT ACAGCTCAGA CTATTACAAT AACACCACCT GAAAAATATG 1441 ATGAGGATGG TATGGACTGC ATGGACAATG AGAGGCGGCC GCATTTCCCT CAATTTTCCT 1501 ACTCTGCAAG TGGACGAGAA TGAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1561 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1621 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CGGGCGGAAG 1681 TCATGTGCGC TCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1741 att