Construct: ORF TRCN0000489919

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020274.1_s317c1
DNA Barcode:
CGAACCTTCGACTGCACGAATTCC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
RPS6KB1 (6198)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489919
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_003161.4 99.8% 99.8% 1575_1576insTTG
2 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001272042.1 95.4% 95.4% 308_309ins69;1506_1507insTTG
3 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001272060.1 95.4% 95.4% 0_1ins69;1506_1507insTTG
4 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369669.1 92.9% 92.9% 1117_1118ins108;1467_1468insTTG
5 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001272044.1 89.7% 89.7% 0_1ins159;1416_1417insTTG
6 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369670.1 85.7% 85.7% 0_1ins159;819_820ins63;1353_1354insTTG
7 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001272043.2 85.5% 84.9% (many diffs)
8 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369671.1 84.6% 84.6% 140_141ins240;1335_1336insTTG
9 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369672.1 82.8% 82.8% 0_1ins159;958_959ins108;1308_1309insTTG
10 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 XM_011525102.3 72.5% 72.2% (many diffs)
11 human 100287072 LOC100287072 ribosomal protein S6 kinase... NR_073509.1 43.3% (many diffs)
12 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369674.1 39.8% 36.5% (many diffs)
13 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369675.1 39.8% 36.5% (many diffs)
14 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369676.1 38.5% 36.5% (many diffs)
15 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369677.1 38.5% 36.5% (many diffs)
16 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369678.1 38.5% 36.5% (many diffs)
17 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369679.1 38.5% 36.5% (many diffs)
18 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161461.1 29% (many diffs)
19 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161456.1 29% (many diffs)
20 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161459.1 28.6% (many diffs)
21 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369673.1 28.4% 26.6% (many diffs)
22 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161458.1 28% (many diffs)
23 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161460.1 27.6% (many diffs)
24 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161457.1 27.2% (many diffs)
25 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161462.1 27% (many diffs)
26 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161455.1 26.3% (many diffs)
27 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 XR_002958051.1 20.8% (many diffs)
28 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... NM_001114334.1 94.4% 99.2% (many diffs)
29 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_006534314.3 84.8% 89.5% (many diffs)
30 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_011249275.2 81.2% 84.6% (many diffs)
31 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_006534315.3 79.2% 84% (many diffs)
32 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_011249276.2 77.1% 78.1% (many diffs)
33 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_006534317.2 58% 61% (many diffs)
34 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_011249277.2 58% 61% (many diffs)
35 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_017314791.1 47.5% 34.7% (many diffs)
36 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_017314792.1 40.4% 34.8% (many diffs)
37 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_017314793.1 36.5% 36.7% (many diffs)
38 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XR_879743.2 33.7% (many diffs)
39 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XR_001780060.1 27.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1650
ORF length:
1578
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaggcga cgaaggaggc gggacggctt ttacccagcc ccggacttcc
121gagacaggga agctgaggac atggcaggag tgtttgacat agacctggac cagccagagg
181acgcgggctc tgaggatgag ctggaggagg ggggtcagtt aaatgaaagc atggaccatg
241ggggagttgg accatatgaa cttggcatgg aacattgtga gaaatttgaa atctcagaaa
301ctagtgtgaa cagagggcca gaaaaaatca gaccagaatg ttttgagcta cttcgggtac
361ttggtaaagg gggctatgga aaggtttttc aagtacgaaa agtaacagga gcaaatactg
421ggaaaatatt tgccatgaag gtgcttaaaa aggcaatgat agtaagaaat gctaaagata
481cagctcatac aaaagcagaa cggaatattc tggaggaagt aaagcatccc ttcatcgtgg
541atttaattta tgcctttcag actggtggaa aactctacct catccttgag tatctcagtg
601gaggagaact atttatgcag ttagaaagag agggaatatt tatggaagac actgcctgct
661tttacttggc agaaatctcc atggctttgg ggcatttaca tcaaaagggg atcatctaca
721gagacctgaa gccggagaat atcatgctta atcaccaagg tcatgtgaaa ctaacagact
781ttggactatg caaagaatct attcatgatg gaacagtcac acacacattt tgtggaacaa
841tagaatacat ggcccctgaa atcttgatga gaagtggcca caatcgtgct gtggattggt
901ggagtttggg agcattaatg tatgacatgc tgactggagc acccccattc actggggaga
961atagaaagaa aacaattgac aaaatcctca aatgtaaact caatttgcct ccctacctca
1021cacaagaagc cagagatctg cttaaaaagc tgctgaaaag aaatgctgct tctcgtctgg
1081gagctggtcc tggggacgct ggagaagttc aagctcatcc attctttaga cacattaact
1141gggaagaact tctggctcga aaggtggagc ccccctttaa acctctgttg caatctgaag
1201aggatgtaag tcagtttgat tccaagttta cacgtcagac acctgtcgac agcccagatg
1261actcaactcT CAGTGAAAGT GCCAATCAGG TCTTTCTGGG TTTTACATAT GTGGCTCCAT
1321CTGTACTTGA AAGTGTGAAA GAAAAGTTTT CCTTTGAACC AAAAATCCGA TCACCTCGAA
1381GATTTATTGG CAGCCCACGA ACACCTGTCA GCCCAGTCAA ATTTTCTCCT GGGGATTTCT
1441GGGGAAGAGG TGCTTCGGCC AGCACAGCAA ATCCTCAGAC ACCTGTGGAA TACCCAATGG
1501AAACAAGTGG CATAGAGCAG ATGGATGTGA CAATGAGTGG GGAAGCATCG GCACCACTTC
1561CAATACGACA GCCGAACTCT GGGCCATACA AAAAACAAGC TTTTCCCATG ATCTCCAAAC
1621GGCCAGAGCA CCTGCGTATG AATCTATTGT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG
1681ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1741GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG
1801AACCTTCGAC TGCACGAATT CCACGCGTTA AGTCgacaat caacctctgg attacaaaat
1861ttgtgaaaga tt

Download FASTA (ORF) (Full)

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