Construct: ORF TRCN0000489919
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020274.1_s317c1
- DNA Barcode:
- CGAACCTTCGACTGCACGAATTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KB1 (6198)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489919
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_003161.4 | 99.8% | 99.8% | 1575_1576insTTG |
| 2 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001272042.1 | 95.4% | 95.4% | 308_309ins69;1506_1507insTTG |
| 3 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001272060.1 | 95.4% | 95.4% | 0_1ins69;1506_1507insTTG |
| 4 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369669.1 | 92.9% | 92.9% | 1117_1118ins108;1467_1468insTTG |
| 5 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001272044.1 | 89.7% | 89.7% | 0_1ins159;1416_1417insTTG |
| 6 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369670.1 | 85.7% | 85.7% | 0_1ins159;819_820ins63;1353_1354insTTG |
| 7 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001272043.2 | 85.5% | 84.9% | (many diffs) |
| 8 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369671.1 | 84.6% | 84.6% | 140_141ins240;1335_1336insTTG |
| 9 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369672.1 | 82.8% | 82.8% | 0_1ins159;958_959ins108;1308_1309insTTG |
| 10 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | XM_011525102.3 | 72.5% | 72.2% | (many diffs) |
| 11 | human | 100287072 | LOC100287072 | ribosomal protein S6 kinase... | NR_073509.1 | 43.3% | (many diffs) | |
| 12 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369674.1 | 39.8% | 36.5% | (many diffs) |
| 13 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369675.1 | 39.8% | 36.5% | (many diffs) |
| 14 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369676.1 | 38.5% | 36.5% | (many diffs) |
| 15 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369677.1 | 38.5% | 36.5% | (many diffs) |
| 16 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369678.1 | 38.5% | 36.5% | (many diffs) |
| 17 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369679.1 | 38.5% | 36.5% | (many diffs) |
| 18 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161461.1 | 29% | (many diffs) | |
| 19 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161456.1 | 29% | (many diffs) | |
| 20 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161459.1 | 28.6% | (many diffs) | |
| 21 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369673.1 | 28.4% | 26.6% | (many diffs) |
| 22 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161458.1 | 28% | (many diffs) | |
| 23 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161460.1 | 27.6% | (many diffs) | |
| 24 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161457.1 | 27.2% | (many diffs) | |
| 25 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161462.1 | 27% | (many diffs) | |
| 26 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161455.1 | 26.3% | (many diffs) | |
| 27 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | XR_002958051.1 | 20.8% | (many diffs) | |
| 28 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | NM_001114334.1 | 94.4% | 99.2% | (many diffs) |
| 29 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_006534314.3 | 84.8% | 89.5% | (many diffs) |
| 30 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_011249275.2 | 81.2% | 84.6% | (many diffs) |
| 31 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_006534315.3 | 79.2% | 84% | (many diffs) |
| 32 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_011249276.2 | 77.1% | 78.1% | (many diffs) |
| 33 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_006534317.2 | 58% | 61% | (many diffs) |
| 34 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_011249277.2 | 58% | 61% | (many diffs) |
| 35 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_017314791.1 | 47.5% | 34.7% | (many diffs) |
| 36 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_017314792.1 | 40.4% | 34.8% | (many diffs) |
| 37 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_017314793.1 | 36.5% | 36.7% | (many diffs) |
| 38 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XR_879743.2 | 33.7% | (many diffs) | |
| 39 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XR_001780060.1 | 27.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1650
- ORF length:
- 1578
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaggcga cgaaggaggc gggacggctt ttacccagcc ccggacttcc 121 gagacaggga agctgaggac atggcaggag tgtttgacat agacctggac cagccagagg 181 acgcgggctc tgaggatgag ctggaggagg ggggtcagtt aaatgaaagc atggaccatg 241 ggggagttgg accatatgaa cttggcatgg aacattgtga gaaatttgaa atctcagaaa 301 ctagtgtgaa cagagggcca gaaaaaatca gaccagaatg ttttgagcta cttcgggtac 361 ttggtaaagg gggctatgga aaggtttttc aagtacgaaa agtaacagga gcaaatactg 421 ggaaaatatt tgccatgaag gtgcttaaaa aggcaatgat agtaagaaat gctaaagata 481 cagctcatac aaaagcagaa cggaatattc tggaggaagt aaagcatccc ttcatcgtgg 541 atttaattta tgcctttcag actggtggaa aactctacct catccttgag tatctcagtg 601 gaggagaact atttatgcag ttagaaagag agggaatatt tatggaagac actgcctgct 661 tttacttggc agaaatctcc atggctttgg ggcatttaca tcaaaagggg atcatctaca 721 gagacctgaa gccggagaat atcatgctta atcaccaagg tcatgtgaaa ctaacagact 781 ttggactatg caaagaatct attcatgatg gaacagtcac acacacattt tgtggaacaa 841 tagaatacat ggcccctgaa atcttgatga gaagtggcca caatcgtgct gtggattggt 901 ggagtttggg agcattaatg tatgacatgc tgactggagc acccccattc actggggaga 961 atagaaagaa aacaattgac aaaatcctca aatgtaaact caatttgcct ccctacctca 1021 cacaagaagc cagagatctg cttaaaaagc tgctgaaaag aaatgctgct tctcgtctgg 1081 gagctggtcc tggggacgct ggagaagttc aagctcatcc attctttaga cacattaact 1141 gggaagaact tctggctcga aaggtggagc ccccctttaa acctctgttg caatctgaag 1201 aggatgtaag tcagtttgat tccaagttta cacgtcagac acctgtcgac agcccagatg 1261 actcaactcT CAGTGAAAGT GCCAATCAGG TCTTTCTGGG TTTTACATAT GTGGCTCCAT 1321 CTGTACTTGA AAGTGTGAAA GAAAAGTTTT CCTTTGAACC AAAAATCCGA TCACCTCGAA 1381 GATTTATTGG CAGCCCACGA ACACCTGTCA GCCCAGTCAA ATTTTCTCCT GGGGATTTCT 1441 GGGGAAGAGG TGCTTCGGCC AGCACAGCAA ATCCTCAGAC ACCTGTGGAA TACCCAATGG 1501 AAACAAGTGG CATAGAGCAG ATGGATGTGA CAATGAGTGG GGAAGCATCG GCACCACTTC 1561 CAATACGACA GCCGAACTCT GGGCCATACA AAAAACAAGC TTTTCCCATG ATCTCCAAAC 1621 GGCCAGAGCA CCTGCGTATG AATCTATTGT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 1681 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1741 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 1801 AACCTTCGAC TGCACGAATT CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1861 ttgtgaaaga tt