Construct: ORF TRCN0000489928
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020242.1_s317c1
- DNA Barcode:
- AAGTCCCACCATTGCGCAATTGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PKN2 (5586)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489928
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5586 | PKN2 | protein kinase N2 | NM_006256.4 | 100% | 100% | |
| 2 | human | 5586 | PKN2 | protein kinase N2 | NM_001320709.2 | 98.3% | 98.3% | 1122_1123ins48 |
| 3 | human | 5586 | PKN2 | protein kinase N2 | NM_001320707.1 | 95.1% | 95.1% | 1280_1281ins144 |
| 4 | human | 5586 | PKN2 | protein kinase N2 | XM_017001782.2 | 93.6% | 93.5% | 985_986ins186 |
| 5 | human | 5586 | PKN2 | protein kinase N2 | XM_017001783.2 | 93.4% | 93.4% | 1122_1123ins48;1232_1233ins144 |
| 6 | human | 5586 | PKN2 | protein kinase N2 | NM_001320708.2 | 84% | 84% | 0_1ins471 |
| 7 | human | 5586 | PKN2 | protein kinase N2 | XM_011541772.2 | 66.8% | 66.5% | 0_1ins946;3G>C;7_8ins32 |
| 8 | mouse | 109333 | Pkn2 | protein kinase N2 | NM_178654.4 | 90.3% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3024
- ORF length:
- 2952
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgtcc aaccccgaac ggggggagat tctgctcacg gaactgcagg 121 gggattcccg aagtcttccg ttttctgaga atgtgagtgc tgttcaaaaa ttagactttt 181 cagatacaat ggtgcagcag aaattggatg atatcaagga tcgaattaag agagaaataa 241 ggaaagaact gaaaatcaaa gaaggagctg aaaatctgag gaaagtcaca acagataaaa 301 aaagtttggc ttatgtagac aacattttga aaaaatcaaa taaaaaatta gaagaactac 361 atcacaagct gcaggaatta aatgcacata ttgttgtatc agatccagaa gatattacag 421 attgcccaag gactccagat actccaaata atgaccctcg ttgttctact agcaacaata 481 gattgaaggc cttacaaaaa caattggata tagaacttaa agtaaaacaa ggtgcagaga 541 atatgataca gatgtattca aatggatctt caaaggatcg gaaactccat ggtacagctc 601 agcaactgct ccaggacagc aagacaaaaa tagaagtcat acgaatgcag attcttcagg 661 cagtccagac taatgaattg gcttttgata atgcaaaacc tgtgataagt cctcttgaac 721 ttcggatgga agaattaagg catcatttta ggatagagtt tgcagtagca gaaggtgcaa 781 agaatgtaat gaaattactt ggctcaggaa aagtaacaga cagaaaagca ctttcagaag 841 ctcaagcaag atttaatgaa tcaagtcaga agttggacct tttaaagtat tcattagagc 901 aaagattaaa cgaagtcccc aagaatcatc ccaaaagcag gattattatt gaagaacttt 961 cacttgttgc tgcatcacca acactaagtc cacgtcaaag tatgatatct acgcaaaatc 1021 aatatagtac actatccaaa ccagcagcac taacaggtac tttggaagtt cgtcttatgg 1081 gctgccaaga tatcctagag aatgtccctg gacggtcaaa agcaacatca gttgcactgc 1141 ctggttggag tccaagtgaa accagatcat ctttcatgag cagaacgagt aaaagtaaaa 1201 gcggaagtag tcgaaatctt ctaaaaaccg atgacttgtc caatgatgtc tgtgctgttt 1261 tgaagctcga taatactgtg gttggccaaa ctagctggaa acccatttcc aatcagtcat 1321 gggaccagaa gtttacactg gaactggaca ggtcacgtga actggaaatt tcagtttatt 1381 ggcgtgattg gcggtctctg tgtgctgtaa aatttctgag gttagaagat tttttagaca 1441 accaacggca tggcatgtgt ctctatttgg aaccacaggg tactttattt gcagaggtta 1501 ccttttttaa tccagttatt gaaagaagac caaaacttca aagacaaaag aaaatttttt 1561 caaagcaaca aggcaaaaca tttctcagag ctcctcaaat gaatattaat attgccactt 1621 ggggaaggct agtaagaaga gctattccta cagtaaatca ttctggcacc ttcagccctc 1681 aagctcctgt gcctactaca gtgccagtgg ttgatgtacg catccctcaa ctagcacctc 1741 cagctagtga ttctacagta accaaattgg actttgatct tgagcctgaa cctcctccag 1801 ccccaccacg agcttcttct cttggagaaa tagatgaatc ttctgaatta agagttttgg 1861 atataccagg acaggattca gagactgttt ttgatattca gaatgacaga aatagtatac 1921 ttccaaaatc tcaatctgaa tacaagcctg atactcctca gtcaggccta gaatatagtg 1981 gtattcaaga acttgaggac agaagatctc agcaaaggtt tcagtttaat ctacaagatt 2041 tcaggtgttg tgctgtcttg ggaagaggac attttggaaa ggtgctttta gctgaatata 2101 aaaacacaaa tgagatgttt gctataaaag ccttaaagaa aggagatatt gtggctcgag 2161 atgaagtaga cagcctgatg tgtgaaaaaa gaatttttga aactgtgaat agtgtaaggc 2221 atcccttttt ggtgaacctt tttgcatgtt tccaaaccaa agagcatgtt tgctttgtaa 2281 tggaatatgc tgccggtggg gacctaatga tgcacattca tactgatgtc ttttctgaac 2341 caagagctgt attttatgct gcttgtgtag ttcttgggtt gcagtattta catgaacaca 2401 aaattgttta tagagatttg aaattggata acttattgct agatacagag ggctttgtga 2461 aaattgctga ttttggtctt tgcaaagaag gaatgggata tggagataga acaagcacat 2521 tttgtggcac tcctgaattt cttgccccag aagtattaac agaaacttct tatacaaggg 2581 ctgtagattg gtggggcctt GGCGTGCTTA TATATGAAAT GCTTGTTGGT GAGTCTCCCT 2641 TTCCTGGTGA TGATGAAGAG GAAGTTTTTG ACAGTATTGT AAATGATGAA GTAAGGTATC 2701 CAAGGTTCTT ATCTACAGAA GCCATTTCTA TAATGAGAAG GCTGTTAAGA AGAAATCCTG 2761 AACGGCGCCT TGGGGCTAGC GAGAAAGATG CAGAGGATGT AAAAAAGCAC CCATTTTTCC 2821 GGCTAATTGA TTGGAGCGCT CTGATGGACA AAAAAGTAAA GCCACCATTT ATACCTACCA 2881 TAAGAGGACG AGAAGATGTT AGTAATTTTG ATGATGAATT TACCTCAGAA GCACCTATTC 2941 TGACTCCACC TCGAGAACCA AGGATACTTT CGGAAGAGGA GCAGGAAATG TTCAGAGATT 3001 TTGACTACAT TGCTGATTGG TGTTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3061 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3121 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAGTCCCA 3181 CCATTGCGCA ATTGCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3241 aagatt