Construct: ORF TRCN0000491781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021696.2_s317c1
- DNA Barcode:
- GATCGAATTTTATTGGACAGATTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ERBB4 (2066)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491781
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003582.1 | 55.4% | .3% | 1_1489del;2619A>G |
| 2 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_005246376.3 | 47.7% | .3% | 1_2035del;3165A>G |
| 3 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | NM_005235.3 | 47.3% | .3% | 1_2065del;3195A>G |
| 4 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_006712364.3 | 46.8% | .3% | 1_2110del;3240A>G |
| 5 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003580.2 | 46.7% | .3% | 1_2113del;3243A>G |
| 6 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_005246377.3 | 46.4% | .3% | 1_2035del;3105_3106ins48;3117A>G |
| 7 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003578.2 | 46.4% | .3% | 1_2143del;3273A>G |
| 8 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | NM_001042599.1 | 46.1% | .3% | 1_2065del;3135_3136ins48;3147A>G |
| 9 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003577.2 | 45.9% | .3% | 1_2188del;3318A>G |
| 10 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003581.2 | 45.2% | .3% | 1_2143del;3213_3214ins48;3225A>G |
| 11 | human | 2066 | ERBB4 | erb-b2 receptor tyrosine ki... | XM_017003579.2 | 44.7% | .3% | 1_2188del;3258_3259ins48;3270A>G |
| 12 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | XM_006495693.3 | 42.7% | .4% | (many diffs) |
| 13 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | XM_006495692.3 | 42.4% | .4% | (many diffs) |
| 14 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | XM_006495694.3 | 41.5% | .4% | (many diffs) |
| 15 | mouse | 13869 | Erbb4 | erb-b2 receptor tyrosine ki... | NM_010154.2 | 41.2% | .4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 262
- ORF end:
- 310
- ORF length:
- 48
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttctt ggaaacagag ttggtggaac cattaactcc cagtggcaca gcacccaatc 121 aagctcaact tcgtattttg aaagaaactg agctgaagag ggtaaaagtc cttggctcag 181 gtgcttttgg aacggtttat aaaggtattt gggtacctga aggagaaact gtgaagattc 241 ctgtggctat taagattctt aatgagacaa ctggtcccaa ggcaaatgtg gagttcatgg 301 atgaagctct gatcatggca agtatggatc atccacacct agtccggttg ctgggtgtgt 361 gtctgagccc aaccatccag ctggttactc aacttatgcc ccatggctgc ctgttggagt 421 atgtccacga gcacaaggat aacattggat cacaactgct gcttaactgg tgtgtccaga 481 tagctaaggg aatgatgtac ctggaagaaa gacgactcgt tcatcgggat ttggcagccc 541 gtaatgtctt agtgaaatct ccaaaccatg tgaaaatcac agattttggg ctagccagac 601 tcttggaagg agatgaaaaa gagtacaatg ctgatggagg aaagatgcca attaaatgga 661 tggctctgga gtgtatacat tacaggaaat tcacccatca gagtgacgtt tggagctatg 721 gagttactat atgggaactg atgacctttg gaggaaaacc ctatgatgga attccaacgc 781 gagaaatccc tgatttatta gagaaaggag aacgtttgcc tcagcctccc atctgcacta 841 ttgacgttta catggtcatg gtcaaatgtt ggatgattga tgctgacagt agacctaaat 901 ttaaggaact ggctgctgag ttttcaagga tggctcgaga ccctcaaaga tacctagtta 961 ttcagggtga tgatcgtatg aagcttccca gtccaaatga cagcaagttc tttcagaatc 1021 tcttggatga agaggatttg gaagatatga tggatgctga ggagtacttg gtccctcagg 1081 ctttcaacat cccacctccc atctatactt ccagagcaag aattgactcg aataggagtg 1141 aaattggaca cagccctcct cctgcctaca cccccatgtc aggaaaccag tttgtgtacc 1201 gagatggagg ttttgctgct gaacaaggag tgtctgtgcc ctacagagcc ccaactagca 1261 caattccaga agctcctgtg gcacagggtg ctactgctga gatttttgat gactcctgct 1321 gtaatggcac cctacgcaag ccagtggcac cccatgtcca agaggacagt agcacccaga 1381 ggtacagtgc tgaccccacc gtgtttgccc cagaacggag cccacgagga gagctggatg 1441 aggaaggtta catgactcct atgcgagaca aacccaaaca agaatacctg aatccagtgg 1501 aggagaaccc ttttgtttct cggagaaaaa atggagacct tcaagcattG GATAATCCCG 1561 AATATCACAA TGCATCCAAT GGTCCACCCA AGGCCGAGGA TGAGTATGTG AATGAGCCAC 1621 TGTACCTCAA CACCTTTGCC AACACCTTGG GAAAAGCTGA GTACCTGAAG AACAACATAC 1681 TGTCAATGCC AGAGAAGGCC AAGAAAGCGT TTGACAACCC TGACTACTGG AACCACAGCC 1741 TGCCACCTCG GAGCACCCTT CAGCACCCAG ACTACCTGCA GGAGTACAGC ACAAAATATT 1801 TTTATAAACA GAATGGGCGG ATCCGGCCTA TTGTGGCAGA GAATCCTGAA TACCTCTCTG 1861 AGTTCTCCCT GAAGCCAGGC ACTGTGCTGC CGCCTCCACC TTACAGACAC CGGAATACTG 1921 TGGTGTAAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1981 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2041 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGATCGA ATTTTATTGG ACAGATTCAC 2101 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt