Construct: ORF TRCN0000492213
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020187.2_s317c1
- DNA Barcode:
- TGTACTCCCGGGACTCAGACCACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NME2 (4831)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492213
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018137.3 | 100% | 100% | |
| 2 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018138.1 | 100% | 100% | |
| 3 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018139.2 | 100% | 100% | |
| 4 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_002512.4 | 100% | 100% | |
| 5 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_000269.2 | 81.4% | 88.1% | (many diffs) |
| 6 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_198175.1 | 69.9% | 75.7% | (many diffs) |
| 7 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NM_001018136.3 | 56.9% | 56.9% | 1_345del |
| 8 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001198682.2 | 53.9% | 49.6% | 227_228ins113;246_247ins97 |
| 9 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NR_037149.2 | 35.8% | 1_673del;1130_1272del | |
| 10 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_001077529.2 | 90.3% | 98% | (many diffs) |
| 11 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_008705.5 | 90.3% | 98% | (many diffs) |
| 12 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | NM_008704.2 | 82.7% | 90.1% | (many diffs) |
| 13 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | XM_030245646.1 | 75.7% | 82.5% | (many diffs) |
| 14 | mouse | 433968 | Gm5566 | predicted pseudogene 5566 | XR_868740.1 | 69.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 528
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccaac ctggagcgca cctTCATCGC CATCAAGCCG GACGGCGTGC 121 AGCGCGGCCT GGTGGGCGAG ATCATCAAGC GCTTCGAGCA GAAGGGATTC CGCCTCGTGG 181 CCATGAAGTT CCTCCGGGCC TCTGAAGAAC ACCTGAAGCA GCACTACATT GACCTGAAAG 241 ACCGACCATT CTTCCCTGGG CTGGTGAAGT ACATGAACTC AGGGCCGGTT GTGGCCATGG 301 TCTGGGAGGG GCTGAACGTG GTGAAGACAG GCCGAGTGAT GCTTGGGGAG ACCAATCCAG 361 CAGATTCAAA GCCAGGCACC ATTCGTGGGG ACTTCTGCAT TCAGGTTGGC AGGAACATCA 421 TTCATGGCAG TGATTCAGTA AAAAGTGCTG AAAAAGAAAT CAGCCTATGG TTTAAGCCTG 481 AAGAACTGGT TGACTACAAG TCTTGTGCTC ATGACTGGGT CTATGAATGA GACCCAGCTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGATGTA CTCCCGGGAC TCAGACCACG ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt