Construct: ORF ccsbBroad304_00322
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012041.1_s304c1, BRDN0000405215
- Derived from:
- ccsbBroadEn_00322
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLCN5 (1184)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00322
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1184 | CLCN5 | chloride voltage-gated chan... | NM_000084.5 | 99.9% | 100% | 609A>G |
| 2 | human | 1184 | CLCN5 | chloride voltage-gated chan... | NM_001282163.1 | 97.3% | 97.3% | 1_60del;669A>G |
| 3 | human | 1184 | CLCN5 | chloride voltage-gated chan... | NM_001127898.4 | 91.3% | 91.4% | 1_210del;819A>G |
| 4 | human | 1184 | CLCN5 | chloride voltage-gated chan... | NM_001127899.4 | 91.3% | 91.4% | 1_210del;819A>G |
| 5 | human | 1184 | CLCN5 | chloride voltage-gated chan... | XM_017029257.1 | 90.9% | 90.9% | 1_222del;831A>G |
| 6 | human | 1184 | CLCN5 | chloride voltage-gated chan... | XM_017029258.1 | 90.9% | 90.9% | 1_222del;831A>G |
| 7 | mouse | 12728 | Clcn5 | chloride channel, voltage-s... | NM_016691.4 | 91.5% | 97.9% | (many diffs) |
| 8 | mouse | 12728 | Clcn5 | chloride channel, voltage-s... | XM_011247446.2 | 84.1% | 90% | (many diffs) |
| 9 | mouse | 12728 | Clcn5 | chloride channel, voltage-s... | NM_001243762.1 | 83.7% | 89.5% | (many diffs) |
| 10 | mouse | 12728 | Clcn5 | chloride channel, voltage-s... | XM_006527562.3 | 82.5% | 88.2% | (many diffs) |
| 11 | mouse | 12728 | Clcn5 | chloride channel, voltage-s... | XM_011247445.2 | 81.4% | 87.1% | (many diffs) |
| 12 | mouse | 12728 | Clcn5 | chloride channel, voltage-s... | XM_011247442.1 | 80.2% | 85.8% | (many diffs) |
| 13 | mouse | 12728 | Clcn5 | chloride channel, voltage-s... | XM_011247443.2 | 80.2% | 85.8% | (many diffs) |
| 14 | mouse | 12728 | Clcn5 | chloride channel, voltage-s... | XM_011247444.2 | 80.2% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2307
- ORF length:
- 2238
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacttcttg gaggagccaa tccctggtgt agggacctat gatgatttca 121 atacaattga ttgggtgaga gagaagtctc gagaccggga taggcaccga gagattacca 181 ataaaagcaa agagtcaaca tgggccttaa ttcacagtgt gagtgatgct ttttccggct 241 ggttgttgat gctccttatt gggcttttat caggttcgtt agctggtttg atagacatct 301 ctgctcattg gatgacagac ttaaaagaag gtatatgcac agggggattc tggtttaacc 361 atgaacattg ttgctggaac tctgagcatg tcacctttga agagagagac aaatgtccag 421 agtggaatag ttggtcccag cttatcatca gcacagatga gggagccttt gcctacatag 481 tcaattattt catgtacgtc ctctgggctc tcctatttgc cttccttgcc gtatctcttg 541 tcaaggtgtt tgcgccttat gcctgtggct ctggaatccc tgagataaaa actatcttga 601 gtggtttcat tattaggggc tatttgggta agtggactct ggttatcaaa accatcacct 661 tggtgctggc agtgtcgtct ggcttgagcc tgggcaaaga gggccctcta gtgcacgtgg 721 cttgctgctg tgggaacatc ctgtgccact gcttcaacaa atacaggaag aatgaagcca 781 agcgcagaga ggtcttgtcg gctgcagcag cagctggtgt atctgtagcc tttggagcac 841 ctataggtgg agtattattc agccttgaag aggtcagcta ctattttccc ctcaaaacat 901 tgtggcgttc attctttgct gccttggtgg cagcattcac tctacgctcc atcaatccat 961 ttgggaacag ccgcctggta ctattttatg tggagtttca caccccatgg catctctttg 1021 agctcgtgcc attcattctg ctgggcatat ttggtggtct gtggggagca ctgtttatcc 1081 gcacaaacat tgcctggtgt cggaagcgaa agaccaccca gttgggcaag tatcctgtta 1141 tagaggtact cgtcgtgaca gccatcactg ccatcctggc tttccccaat gaatacactc 1201 ggatgagcac aagtgagctc atttctgagc tgtttaatga ctgtggcctt ctggactcct 1261 ccaagctctg tgattatgag aaccgtttca acacaagcaa agggggtgaa ctgcctgaca 1321 gaccggctgg cgtgggagtc tacagtgcaa tgtggcagct ggctttaaca ctcatactga 1381 aaattgtcat tactatattc acctttggca tgaagatccc ttctggcctc tttatcccta 1441 gcatggctgt tggtgctata gcaggtcgac ttctaggagt aggaatggaa cagctggctt 1501 attaccacca ggaatggacc gtcttcaata gctggtgtag tcagggagct gattgcatca 1561 cccccggcct ttatgcaatg gttggggctg cagcctgctt aggtggggtg actcggatga 1621 ctgtttctct tgttgtcata atgtttgaac tgactggtgg cttagaatac atcgtgcctc 1681 tgatggctgc agccatgaca agcaagtggg tggcagatgc tcttgggcgg gagggcatct 1741 atgatgccca catccgtctc aatggatacc cctttcttga agccaaagaa gagtttgctc 1801 ataagaccct ggcaatggat gtgatgaaac cccggagaaa tgatcctttg ttgactgtcc 1861 ttactcagga cagtatgact gtggaagatg tagagaccat aatcagtgaa accacttaca 1921 gtggcttccc agtggtggta tcccgggagt cccaaagact tgtgggcttt gtcctccgaa 1981 gagatctcat tatttcaatt gaaaatgctc gaaagaaaca ggatggggtt gttagcactt 2041 ccatcattta tttcacggag cattctcctc cattgccacc atacactcca cccactctaa 2101 agcttcggaa catcctcgat ctcagcccct tcactgtgac tgaccttaca cccatggaga 2161 tcgtagtgga tattttccga aagctgggac tgcggcagtg cctggttaca cacaacgggc 2221 gattgcttgg aatcattacc aaaaaggatg tgttaaagca tatagcacag atggcgaacc 2281 aagatcctga ttccattctc ttcaacttgc caactttctt gtacaaagtg gttggtaagc 2341 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 2401 ggcgcgttaa gtcgacaatc aacctctgga tta