Construct: ORF ccsbBroad304_02397
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003298.1_s304c1, BRDN0000402228
- Derived from:
- ccsbBroadEn_02397
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTN3 (10313)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02397
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10313 | RTN3 | reticulon 3 | NM_006054.4 | 100% | 100% | |
| 2 | human | 10313 | RTN3 | reticulon 3 | NM_201429.2 | 92.5% | 92.5% | 142_198del |
| 3 | human | 10313 | RTN3 | reticulon 3 | NM_201430.3 | 82.9% | 78.9% | 606_607ins59;650_723del |
| 4 | human | 10313 | RTN3 | reticulon 3 | XM_011544731.2 | 77.3% | 73.5% | 142_198del;663_664ins59;707_780del |
| 5 | human | 10313 | RTN3 | reticulon 3 | NM_001265591.2 | 50.3% | 44.1% | 350_351ins256;453_642del |
| 6 | human | 10313 | RTN3 | reticulon 3 | NM_001265590.2 | 23.5% | 20.6% | (many diffs) |
| 7 | human | 10313 | RTN3 | reticulon 3 | NR_049750.2 | 22.4% | 1_137del;487_488ins139;707_2390del | |
| 8 | human | 10313 | RTN3 | reticulon 3 | NM_201428.3 | 21.3% | 18.7% | (many diffs) |
| 9 | human | 10313 | RTN3 | reticulon 3 | NM_001265589.2 | 20.9% | 18.4% | (many diffs) |
| 10 | human | 10313 | RTN3 | reticulon 3 | NR_049751.2 | 20.1% | (many diffs) | |
| 11 | human | 10313 | RTN3 | reticulon 3 | XM_011544730.2 | 18.6% | 15.2% | (many diffs) |
| 12 | mouse | 20168 | Rtn3 | reticulon 3 | NM_053076.3 | 90.7% | 94.9% | (many diffs) |
| 13 | mouse | 20168 | Rtn3 | reticulon 3 | NM_001271487.1 | 83.9% | 87.8% | (many diffs) |
| 14 | mouse | 20168 | Rtn3 | reticulon 3 | XM_017318088.1 | 77.7% | 79.3% | (many diffs) |
| 15 | mouse | 20168 | Rtn3 | reticulon 3 | NM_001271486.1 | 30.3% | 31.2% | (many diffs) |
| 16 | mouse | 20168 | Rtn3 | reticulon 3 | NM_001003933.2 | 20.8% | 21.2% | (many diffs) |
| 17 | mouse | 20168 | Rtn3 | reticulon 3 | NM_001003934.2 | 20.3% | 20.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagccgtcg gcggccactc agtcccattc catctcctcg tcgtccttcg 121 gagccgagcc gtccgcgccc ggcggcggcg ggagcccagg agcctgcccc gccctgggga 181 cgaagagctg cagctcctcc tgtgcggtgc acgatctgat tttctggaga gatgtgaaga 241 agactgggtt tgtctttggc accacgctga tcatgctgct ttccctggca gctttcagtg 301 tcatcagtgt ggtttcttac ctcatcctgg ctcttctctc tgtcaccatc agcttcagga 361 tctacaagtc cgtcatccaa gctgtacaga agtcagaaga aggccatcca ttcaaagcct 421 acctggacgt agacattact ctgtcctcag aagctttcca taattacatg aatgctgcca 481 tggtgcacat caacagggcc ctgaaactca ttattcgtct ctttctggta gaagatctgg 541 ttgactcctt gaagctggct gtcttcatgt ggctgatgac ctatgttggt gctgttttta 601 acggaatcac ccttctaatt cttgctgaac tgctcatttt cagtgtcccg attgtctatg 661 agaagtacaa gacccagatt gatcactatg ttggcatcgc ccgagatcag accaagtcaa 721 ttgttgaaaa gatccaagca aaactccctg gaatcgccaa aaaaaaggca gaatacccaa 781 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 841 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta