Transcript: Mouse XM_006530650.3

PREDICTED: Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 19a (Ddx19a), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ddx19a (13680)
Length:
2772
CDS:
128..1294

Additional Resources:

NCBI RefSeq record:
XM_006530650.3
NBCI Gene record:
Ddx19a (13680)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006530650.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000096845 CCCAACATATGAGCTGGCATT pLKO.1 361 CDS 100% 0.000 0.000 N Ddx19a n/a
2 TRCN0000307630 TCTACGCCATGGGCTTCAATC pLKO_005 174 CDS 100% 10.800 8.640 N Ddx19a n/a
3 TRCN0000096844 CCTTGAATAAATGAGAGGAAA pLKO.1 1776 3UTR 100% 4.950 3.465 N Ddx19a n/a
4 TRCN0000288480 CCTTGAATAAATGAGAGGAAA pLKO_005 1776 3UTR 100% 4.950 3.465 N Ddx19a n/a
5 TRCN0000096846 AGCTGGCATTACAAACTGGAA pLKO.1 372 CDS 100% 2.640 1.848 N Ddx19a n/a
6 TRCN0000295802 GTGGAACCAGCAGACAGATAT pLKO_005 320 CDS 100% 13.200 7.920 N Ddx19a n/a
7 TRCN0000096848 CCCAAGAAGATCAAGGTGTTT pLKO.1 551 CDS 100% 4.950 2.475 Y Ddx19a n/a
8 TRCN0000151004 GATTTGGACGAGATTGAGAAA pLKO.1 1262 CDS 100% 4.950 2.475 Y DDX19A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006530650.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02664 pDONR223 100% 85.3% 92.7% None (many diffs) n/a
2 ccsbBroad304_02664 pLX_304 0% 85.3% 92.7% V5 (many diffs) n/a
3 TRCN0000492319 TCACCATTCCTGCGTCCACGACGC pLX_317 2.8% 85.3% 92.7% V5 (many diffs) n/a
4 ccsbBroadEn_03576 pDONR223 100% 72.9% 78.4% None (many diffs) n/a
5 ccsbBroad304_03576 pLX_304 0% 72.9% 78.4% V5 (many diffs) n/a
6 TRCN0000472365 CATCTACGCACTTAACAACACTCT pLX_317 29.8% 72.9% 78.4% V5 (many diffs) n/a
7 ccsbBroadEn_02663 pDONR223 100% 72.2% 77.6% None (many diffs) n/a
8 ccsbBroad304_02663 pLX_304 0% 72.2% 77.6% V5 (many diffs) n/a
9 TRCN0000472192 ATGCACTATTTATGCCGATATGCA pLX_317 32.3% 72.2% 77.6% V5 (many diffs) n/a
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