Transcript: Mouse NM_001190800.1

Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 19b (Ddx19b), transcript variant 3, mRNA.

Source:
NCBI, updated 2015-02-15
Taxon:
Mus musculus (mouse)
Gene:
Ddx19b (234733)
Length:
6634
CDS:
117..1520

Additional Resources:

NCBI RefSeq record:
NM_001190800.1
NBCI Gene record:
Ddx19b (234733)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001190800.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000096791 GCTGTTGTTAAGACCAATGCA pLKO.1 225 CDS 100% 3.000 2.400 N Ddx19b n/a
2 TRCN0000051129 CCTGAACTGAAGCTAGCTTAT pLKO.1 684 CDS 100% 10.800 7.560 N DDX19B n/a
3 TRCN0000365062 CTGAACTGAAGCTAGCTTATG pLKO_005 685 CDS 100% 10.800 7.560 N DDX19B n/a
4 TRCN0000096789 GCTGACATTCTTTCCAGGAAA pLKO.1 2080 3UTR 100% 4.950 3.465 N Ddx19b n/a
5 TRCN0000096792 TGTTGTTAAGACCAATGCAAA pLKO.1 227 CDS 100% 4.950 3.465 N Ddx19b n/a
6 TRCN0000096790 CCAACCTATGAGCTTGCTCTT pLKO.1 624 CDS 100% 4.050 2.835 N Ddx19b n/a
7 TRCN0000096793 AGGGAGTCTATGCTATGGGTT pLKO.1 430 CDS 100% 2.640 1.848 N Ddx19b n/a
8 TRCN0000096848 CCCAAGAAGATCAAGGTGTTT pLKO.1 813 CDS 100% 4.950 2.475 Y Ddx19a n/a
9 TRCN0000151004 GATTTGGACGAGATTGAGAAA pLKO.1 1519 CDS 100% 4.950 2.475 Y DDX19A n/a
10 TRCN0000051130 CACAGAACTTAATTGCCCAAT pLKO.1 508 CDS 100% 4.050 2.835 N DDX19B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001190800.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02663 pDONR223 100% 87.8% 93.1% None (many diffs) n/a
2 ccsbBroad304_02663 pLX_304 0% 87.8% 93.1% V5 (many diffs) n/a
3 TRCN0000472192 ATGCACTATTTATGCCGATATGCA pLX_317 32.3% 87.8% 93.1% V5 (many diffs) n/a
4 ccsbBroadEn_03576 pDONR223 100% 85.8% 89.1% None (many diffs) n/a
5 ccsbBroad304_03576 pLX_304 0% 85.8% 89.1% V5 (many diffs) n/a
6 TRCN0000472365 CATCTACGCACTTAACAACACTCT pLX_317 29.8% 85.8% 89.1% V5 (many diffs) n/a
7 ccsbBroadEn_02664 pDONR223 100% 67.5% 71.5% None (many diffs) n/a
8 ccsbBroad304_02664 pLX_304 0% 67.5% 71.5% V5 (many diffs) n/a
9 TRCN0000492319 TCACCATTCCTGCGTCCACGACGC pLX_317 2.8% 67.5% 71.5% V5 (many diffs) n/a
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