Construct: ORF ccsbBroad304_04249

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013801.1_s304c1, BRDN0000410627
Derived from:
ccsbBroadEn_04249
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DIAPH3 (81624)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04249
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81624 DIAPH3 diaphanous related formin 3 NM_030932.3 100% 100%
2 human 81624 DIAPH3 diaphanous related formin 3 XM_006719876.1 91% 90.7% (many diffs)
3 human 81624 DIAPH3 diaphanous related formin 3 NM_001258370.2 79.7% 76.9% (many diffs)
4 human 81624 DIAPH3 diaphanous related formin 3 NM_001258369.1 76.3% 76.3% 1_789del
5 human 81624 DIAPH3 diaphanous related formin 3 NM_001258368.2 75.3% 75.1% (many diffs)
6 human 81624 DIAPH3 diaphanous related formin 3 XM_011535258.2 74.3% 74.3% (many diffs)
7 human 81624 DIAPH3 diaphanous related formin 3 XM_024449422.1 74% 74% (many diffs)
8 human 81624 DIAPH3 diaphanous related formin 3 NM_001258367.2 73.8% 73.5% (many diffs)
9 human 81624 DIAPH3 diaphanous related formin 3 NM_001258366.2 71.6% 71.4% (many diffs)
10 human 81624 DIAPH3 diaphanous related formin 3 NM_001042517.2 70.9% 70.7% (many diffs)
11 human 81624 DIAPH3 diaphanous related formin 3 XR_001749694.1 66.4% 1_951del;2426_2447del;3313_3314ins207
12 human 81624 DIAPH3 diaphanous related formin 3 XM_011535263.2 41% 39% (many diffs)
13 human 81624 DIAPH3 diaphanous related formin 3 XM_011535265.2 40% 39% (many diffs)
14 human 81624 DIAPH3 diaphanous related formin 3 XM_017020789.1 39.3% 38.6% (many diffs)
15 human 81624 DIAPH3 diaphanous related formin 3 XR_941672.1 38.8% (many diffs)
16 human 81624 DIAPH3 diaphanous related formin 3 XR_002957480.1 36.2% (many diffs)
17 human 81624 DIAPH3 diaphanous related formin 3 XR_002957477.1 31.6% (many diffs)
18 human 81624 DIAPH3 diaphanous related formin 3 XR_002957479.1 31.5% (many diffs)
19 human 81624 DIAPH3 diaphanous related formin 3 XR_002957478.1 31.5% (many diffs)
20 mouse 56419 Diaph3 diaphanous related formin 3 XM_006519312.3 64.3% 62.1% (many diffs)
21 mouse 56419 Diaph3 diaphanous related formin 3 XM_011245123.2 63.4% 62.3% (many diffs)
22 mouse 56419 Diaph3 diaphanous related formin 3 NM_019670.1 62.8% 61.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2613
ORF length:
2547
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag tgaggagagg agcctttcct tattggccaa agccgtggat cccagacacc
121ccaatatgat gacagatgtg gttaaacttc tctctgcggt atgcattgta ggggaagaaa
181gcatccttga agaagtttta gaagctttaa cttcagctgg tgaagaaaaa aaaattgaca
241gatttttttg tattgtggaa ggcctccggc acaattcagt tcaactgcaa gtagcttgta
301tgcagctcat caatgccctg gttacatctc ctgatgattt ggatttcagg cttcacatca
361gaaatgaatt tatgcgttgt ggattgaaag agatattgcc aaatttaaaa tgcattaaga
421atgatggcct ggatatccaa cttaaagtct ttgatgagca taaagaagaa gatttgtttg
481agttatccca tcgccttgaa gatattagag ctgaacttga tgaagcatat gatgtttaca
541acatggtgtg gagcacagtt aaagaaacta gagcagaggg atattttatt tctattcttc
601agcatctttt gctgattcga aatgattatt ttataaggca acaatacttc aaattaattg
661atgagtgtgt atcccagatt gtattgcata gagatggaat ggatccagac ttcacatatc
721gaaaaagact agatttagat ttaacccagt ttgtagacat ttgcatagat caagcaaaac
781tagaagagtt tgaagagaaa gcatcagaac tttacaagaa atttgaaaaa gagtttaccg
841accaccaaga aactcaggct gaattgcaga aaaaagaggc aaagattaat gagcttcaag
901cagagctaca agcttttaag tctcagtttg gtgccttgcc agctgattgt aatattcctt
961tgcctccctc taaagaaggt ggaactggcc actcagcact tcctcctccg cctccactgc
1021cttctggtgg aggggtgccg cctccacctc ctcccccacc acctcctcca cttccaggaa
1081tgcggatgcc attcagtggt cctgtgcctc caccacctcc cctgggattc cttggaggac
1141aaaattctcc tcctctacca atcctgccat ttgggttgaa accaaagaaa gaatttaaac
1201ctgaaatcag catgagaaga ttgaattggt taaagatcag acctcatgaa atgactgaaa
1261actgtttctg gataaaagta aatgaaaata agtatgaaaa cgtggatttg ctttgtaaac
1321ttgagaatac attttgttgc caacaaaaag agagaagaga agaggaagat attgaagaga
1381agaaatcgat taagaaaaaa attaaagaac ttaagttttt agattctaaa attgcccaga
1441acctttcaat cttcctgagc tcttttcggg tgccatatga ggaaatcaga atgatgatat
1501tggaagtaga tgaaacacgg ttggcagagt ctatgattca gaacttaata aagcatcttc
1561ctgatcaaga gcaattaaat tcattgtctc agttcaagag tgaatatagc aacttatgtg
1621aacctgagca gtttgtggtt gtgatgagca atgtgaagag actacggcca cggctcagtg
1681ctattctctt taagcttcag tttgaagagc aggtgaacaa catcaaacct gacatcatgg
1741ctgtcagtac tgcctgcgaa gagataaaga agagcaaaag ctttagcaag ttgctggaac
1801ttgtattgct aatgggaaac tacatgaatg ctggctcccg gaatgctcaa accttcggat
1861ttaaccttag ctctctctgt aaactaaagg acacaaaatc agcagatcag aaaacaacgc
1921tacttcattt cctggtagaa atatgtgaag agaagtaccc tgatatactg aattttgtgg
1981atgatttgga acctttagac aaagctagta aagtctctgt agaaacgctg gaaaagaatt
2041tgaggcagat gggaaggcag cttcaacagc ttgagaagga attggaaacc tttccccctc
2101ctgaggactt gcatgacaag tttgtgacaa agatgtccag atttgttatc agtgcaaaag
2161aacaatatga gacactttcg aagttacacg aaaacatgga aaagttatac cagagtataa
2221taggatacta tgccattgat gtgaagaagg tgtctgtgga agactttctt actgacctga
2281ataacttcag aaccacattc atgcaagcaa taaaggagaa tatcaaaaaa agagaagcag
2341aggaaaaaga aaaacgtgtc agaatagcta aagaattagc agagcgagaa agactcgaac
2401gccaacaaaa gaaaaagcgt ttattagaaa tgaagactga gggtgatgag acaggagtga
2461tggataatct gctggaggcc ttgcagtccg gggctgcctt ccgcgacaga agaaaaagga
2521caccgatgcc aaaagatgtt cggcagagtc tcagtccaat gtctcagagg cctgttctga
2581aagtttgtaa ccatggtaat aaaccgtatt tatacccaac tttcttgtac aaagtggttg
2641gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2701accggtggcg cgttaagtcg acaatcaacc tctggatta

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