Construct: ORF ccsbBroad304_04404
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000654.1_s304c1, BRDN0000410562
- Derived from:
- ccsbBroadEn_04404
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBB6 (84617)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04404
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303524.1 | 100% | 100% | |
2 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_032525.3 | 100% | 100% | |
3 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303526.2 | 91.7% | 91.4% | 166_167ins111 |
4 | human | 10381 | TUBB3 | tubulin beta 3 class III | NM_006086.4 | 87.7% | 91.5% | (many diffs) |
5 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289129.1 | 86.3% | 91.7% | (many diffs) |
6 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_006087.4 | 86.3% | 91.7% | (many diffs) |
7 | human | 7280 | TUBB2A | tubulin beta 2A class IIa | NM_001069.3 | 86.2% | 90.5% | (many diffs) |
8 | human | 347733 | TUBB2B | tubulin beta 2B class IIb | NM_178012.5 | 86.1% | 90.8% | (many diffs) |
9 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303528.2 | 83.8% | 77.7% | (many diffs) |
10 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303527.2 | 83.8% | 83.8% | 0_1ins216 |
11 | human | 10383 | TUBB4B | tubulin beta 4B class IVb | NM_006088.6 | 83.2% | 90.5% | (many diffs) |
12 | human | 203068 | TUBB | tubulin beta class I | NM_178014.4 | 80.9% | 90.3% | (many diffs) |
13 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289127.1 | 78.5% | 83.3% | (many diffs) |
14 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289123.1 | 77.5% | 82.3% | (many diffs) |
15 | human | 203068 | TUBB | tubulin beta class I | NM_001293212.2 | 75.9% | 83.6% | (many diffs) |
16 | human | 10381 | TUBB3 | tubulin beta 3 class III | NM_001197181.2 | 74.7% | 77.3% | (many diffs) |
17 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289130.1 | 74.2% | 78.2% | (many diffs) |
18 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289131.1 | 74.2% | 78.2% | (many diffs) |
19 | human | 203068 | TUBB | tubulin beta class I | NM_001293214.2 | 74% | 82.9% | (many diffs) |
20 | human | 7280 | TUBB2A | tubulin beta 2A class IIa | NM_001310315.2 | 71.1% | 72.6% | (many diffs) |
21 | human | 203068 | TUBB | tubulin beta class I | NM_001293215.2 | 69% | 76.9% | (many diffs) |
22 | human | 203068 | TUBB | tubulin beta class I | NM_001293216.2 | 69% | 76.9% | (many diffs) |
23 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303529.2 | 67.2% | 67.2% | 0_1ins438 |
24 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303530.2 | 67.2% | 67.2% | 0_1ins438 |
25 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_011519459.3 | 67% | 72.4% | (many diffs) |
26 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_017016192.2 | 59.9% | 63.9% | (many diffs) |
27 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_017016193.2 | 59.9% | 63.9% | (many diffs) |
28 | human | 260334 | TUBB8B | tubulin beta 8B | XM_024451147.1 | 59% | 63.2% | (many diffs) |
29 | human | 203068 | TUBB | tubulin beta class I | NM_001293213.2 | 43.6% | 47% | (many diffs) |
30 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303525.1 | 22.5% | 20.8% | (many diffs) |
31 | mouse | 67951 | Tubb6 | tubulin, beta 6 class V | NM_026473.2 | 89.7% | 97.3% | (many diffs) |
32 | mouse | 22152 | Tubb3 | tubulin, beta 3 class III | NM_023279.2 | 85.8% | 91.3% | (many diffs) |
33 | mouse | 73710 | Tubb2b | tubulin, beta 2B class IIB | NM_023716.2 | 84.7% | 90.8% | (many diffs) |
34 | mouse | 22151 | Tubb2a | tubulin, beta 2A class IIA | NM_009450.2 | 83.3% | 90.5% | (many diffs) |
35 | mouse | 22153 | Tubb4a | tubulin, beta 4A class IVA | NM_009451.3 | 82.1% | 91.7% | (many diffs) |
36 | mouse | 22154 | Tubb5 | tubulin, beta 5 class I | NM_011655.5 | 80.5% | 90.3% | (many diffs) |
37 | mouse | 227613 | Tubb4b | tubulin, beta 4B class IVB | NM_146116.2 | 80.2% | 90.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1404
- ORF length:
- 1338
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ggagatcgtg cacatccagg cgggccagtg cgggaaccag atcggcacca 121 agttttggga agtgatcagc gatgagcacg gcatcgaccc ggccggaggc tacgtgggag 181 actcggcgct gcagctggag agaatcaacg tctactacaa tgagtcatcg tctcagaaat 241 atgtgcccag ggccgccctg gtggacttag agccaggcac catggacagc gtgcggtctg 301 ggccttttgg gcagcttttc cggcctgaca acttcatctt tggccagacg ggtgcaggga 361 acaactgggc gaaagggcac tacacggagg gcgcggagct ggtggacgca gtgctggacg 421 tggtgcggaa ggagtgcgag cactgcgact gcctgcaggg cttccagctc acgcactcgc 481 tgggcggcgg cacgggctca ggcatgggca cgctgctcat cagcaagatc cgtgaggagt 541 tcccggaccg catcatgaac accttcagcg tcatgccctc gcccaaggtg tcggacacgg 601 tggtggagcc ctacaatgcc acactgtcgg tgcaccagct ggtggagaat acagacgaga 661 cctactgcat cgacaacgag gcgctctatg acatctgctt ccgcactctg aagctgacaa 721 cgcccaccta cggggacctc aaccacctgg tgtccgccac catgagtggg gtcaccacct 781 cgctgcgctt cccgggccag ctcaatgctg acctgcgcaa gctggcggtg aacatggtgc 841 ccttcccgcg cctgcacttc ttcatgcctg gcttcgcgcc gctcaccagc cgcggcagcc 901 agcagtaccg ggccctgacc gtgcccgagc tcacccagca gatgttcgac gccaggaaca 961 tgatggccgc ctgcgatccg cgccatggcc gctacctgac cgtggccacc gtgttccgcg 1021 ggcccatgtc catgaaggag gtggacgagc agatgctggc catccagagt aagaacagca 1081 gctacttcgt ggagtggatt cccaacaacg tgaaggtggc cgtgtgcgac atcccgcccc 1141 gcggcctgaa gatggcctcc accttcatcg gcaacagcac ggccatccag gagctgttca 1201 agcgcatctc cgagcagttc tcagccatgt tccggcgcaa ggccttcctg cactggttca 1261 cgggtgaggg catggatgaa atggagttca ccgaggcgga gagcaacatg aacgacctgg 1321 tatccgagta ccagcagtac caggatgcca ccgccaatga cggggaggaa gcttttgagg 1381 atgaggaaga ggagatcgat ggatacccaa ctttcttgta caaagtggtt ggtaagccta 1441 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1501 gcgttaagtc gacaatcaac ctctggatta