Transcript: Mouse NM_023716.2

Mus musculus tubulin, beta 2B class IIB (Tubb2b), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Tubb2b (73710)
Length:
1922
CDS:
122..1459

Additional Resources:

NCBI RefSeq record:
NM_023716.2
NBCI Gene record:
Tubb2b (73710)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_023716.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000090514 GCATCGATAATGAGGCTCTGT pLKO.1 723 CDS 100% 2.640 1.848 N Tubb2b n/a
2 TRCN0000090513 CCACCTTTCTGAACACAGAAA pLKO.1 1771 3UTR 100% 0.495 0.347 N Tubb2b n/a
3 TRCN0000090516 TGGACCATTTGGGCAGATCTT pLKO.1 355 CDS 100% 4.950 2.970 N Tubb2b n/a
4 TRCN0000090515 ACTACAATGAAGCAACTGGTA pLKO.1 270 CDS 100% 2.640 1.584 N Tubb2b n/a
5 TRCN0000089932 GCAGAACAAGAACAGCAGCTA pLKO.1 1120 CDS 100% 2.640 1.320 Y Tubb2a n/a
6 TRCN0000090517 GTGCAGGAAATAACTGGGCAA pLKO.1 408 CDS 100% 2.160 1.080 Y Tubb2b n/a
7 TRCN0000029134 GCAACATGAATGACCTGGTAT pLKO.1 1359 CDS 100% 4.950 2.475 Y TUBB4A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_023716.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05511 pDONR223 100% 91.5% 100% None (many diffs) n/a
2 ccsbBroad304_05511 pLX_304 0% 91.5% 100% V5 (many diffs) n/a
3 TRCN0000478420 AGGACATGACATCGGGCAGTTTCG pLX_317 18.3% 91.5% 100% V5 (many diffs) n/a
4 ccsbBroadEn_07109 pDONR223 100% 91.3% 99.5% None (many diffs) n/a
5 ccsbBroad304_07109 pLX_304 0% 91.3% 99.5% V5 (many diffs) n/a
6 TRCN0000470494 CAATTACAACATTCATCTATATAC pLX_317 29.5% 91.3% 99.5% V5 (many diffs) n/a
7 ccsbBroadEn_07591 pDONR223 100% 87.4% 95.5% None (many diffs) n/a
8 ccsbBroad304_07591 pLX_304 0% 87.4% 95.5% V5 (many diffs) n/a
9 TRCN0000472336 CCGGAACAAGTAAACTGCTTATCA pLX_317 41.4% 87.4% 95.5% V5 (many diffs) n/a
10 ccsbBroadEn_02416 pDONR223 100% 85.4% 96.8% None (many diffs) n/a
11 ccsbBroad304_02416 pLX_304 0% 85.4% 96.8% V5 (many diffs) n/a
12 TRCN0000466709 TCTCGCAGGACAGTTCGGCCAACT pLX_317 32.4% 85.4% 96.8% V5 (many diffs) n/a
13 ccsbBroadEn_04404 pDONR223 100% 84.7% 90.8% None (many diffs) n/a
14 ccsbBroad304_04404 pLX_304 0% 84.7% 90.8% V5 (many diffs) n/a
15 TRCN0000480164 TGTCGAGACCCGACGGGAATTTTT pLX_317 24.4% 84.7% 90.8% V5 (many diffs) n/a
16 ccsbBroadEn_05206 pDONR223 100% 84.5% 95.7% None (many diffs) n/a
17 ccsbBroad304_05206 pLX_304 0% 84.5% 95.7% V5 (many diffs) n/a
18 ccsbBroadEn_02415 pDONR223 100% 84.2% 91.3% None (many diffs) n/a
19 ccsbBroad304_02415 pLX_304 0% 84.2% 91.3% V5 (many diffs) n/a
20 TRCN0000469802 TTTCCTGATTTATTTGACTTAATT pLX_317 33.2% 84.2% 91.3% V5 (many diffs) n/a
21 TRCN0000488922 TAACTAAGCCACAGTTTAACTCGA pLX_317 26.8% 84.2% 91.3% V5 (not translated due to prior stop codon) (many diffs) n/a
22 ccsbBroadEn_13398 pDONR223 100% 25% 25.8% None (many diffs) n/a
23 ccsbBroad304_13398 pLX_304 0% 25% 25.8% V5 (many diffs) n/a
24 TRCN0000466902 ACCACAGTACACCCTTGCTTCGCA pLX_317 100% 25% 25.8% V5 (many diffs) n/a
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