Transcript: Mouse NM_146116.2

Mus musculus tubulin, beta 4B class IVB (Tubb4b), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Tubb4b (227613)
Length:
1600
CDS:
113..1450

Additional Resources:

NCBI RefSeq record:
NM_146116.2
NBCI Gene record:
Tubb4b (227613)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_146116.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000091910 CTGCGGAAACTGGCTGTAAAT pLKO.1 860 CDS 100% 13.200 9.240 N Tubb4b-ps1 n/a
2 TRCN0000090276 GCTATTCAGGAGCTGTTCAAA pLKO.1 1229 CDS 100% 5.625 3.938 N Tubb4b n/a
3 TRCN0000436942 GAGCGCATCAACGTGTACTAC pLKO_005 245 CDS 100% 4.950 3.465 N TUBB8 n/a
4 TRCN0000090277 GCGCATCAACGTGTACTACAA pLKO.1 247 CDS 100% 4.950 3.465 N Tubb4b n/a
5 TRCN0000090273 GCTGTCTTAGTCACTAAAGCA pLKO.1 1452 3UTR 100% 3.000 2.100 N Tubb4b n/a
6 TRCN0000090274 CCAGCAGATGTTTGATGCCAA pLKO.1 982 CDS 100% 2.640 1.848 N Tubb4b n/a
7 TRCN0000090275 GCAGATCTTCAGACCTGATAA pLKO.1 358 CDS 100% 13.200 7.920 N Tubb4b n/a
8 TRCN0000113920 CGCATCAACGTGTACTACAAT pLKO.1 248 CDS 100% 5.625 3.938 N TUBB4B n/a
9 TRCN0000290838 CGCATCAACGTGTACTACAAT pLKO_005 248 CDS 100% 5.625 3.938 N TUBB4B n/a
10 TRCN0000116640 CCCAGCAGATGTTTGATGCTA pLKO.1 981 CDS 100% 3.000 2.100 N TUBB8 n/a
11 TRCN0000091911 GCTGAGGAAGAGGGAGAGTAT pLKO.1 1400 CDS 100% 4.950 2.970 N Tubb4b-ps1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_146116.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02416 pDONR223 100% 90.1% 100% None (many diffs) n/a
2 ccsbBroad304_02416 pLX_304 0% 90.1% 100% V5 (many diffs) n/a
3 TRCN0000466709 TCTCGCAGGACAGTTCGGCCAACT pLX_317 32.4% 90.1% 100% V5 (many diffs) n/a
4 ccsbBroadEn_07109 pDONR223 100% 85.2% 96.4% None (many diffs) n/a
5 ccsbBroad304_07109 pLX_304 0% 85.2% 96.4% V5 (many diffs) n/a
6 TRCN0000470494 CAATTACAACATTCATCTATATAC pLX_317 29.5% 85.2% 96.4% V5 (many diffs) n/a
7 ccsbBroadEn_05511 pDONR223 100% 84.9% 96.8% None (many diffs) n/a
8 ccsbBroad304_05511 pLX_304 0% 84.9% 96.8% V5 (many diffs) n/a
9 TRCN0000478420 AGGACATGACATCGGGCAGTTTCG pLX_317 18.3% 84.9% 96.8% V5 (many diffs) n/a
10 ccsbBroadEn_07591 pDONR223 100% 84.3% 98.4% None (many diffs) n/a
11 ccsbBroad304_07591 pLX_304 0% 84.3% 98.4% V5 (many diffs) n/a
12 TRCN0000472336 CCGGAACAAGTAAACTGCTTATCA pLX_317 41.4% 84.3% 98.4% V5 (many diffs) n/a
13 ccsbBroadEn_05206 pDONR223 100% 83.1% 97.3% None (many diffs) n/a
14 ccsbBroad304_05206 pLX_304 0% 83.1% 97.3% V5 (many diffs) n/a
15 ccsbBroadEn_02415 pDONR223 100% 81.7% 91.7% None (many diffs) n/a
16 ccsbBroad304_02415 pLX_304 0% 81.7% 91.7% V5 (many diffs) n/a
17 TRCN0000469802 TTTCCTGATTTATTTGACTTAATT pLX_317 33.2% 81.7% 91.7% V5 (many diffs) n/a
18 TRCN0000488922 TAACTAAGCCACAGTTTAACTCGA pLX_317 26.8% 81.7% 91.7% V5 (not translated due to prior stop codon) (many diffs) n/a
19 ccsbBroadEn_04404 pDONR223 100% 80.2% 90.5% None (many diffs) n/a
20 ccsbBroad304_04404 pLX_304 0% 80.2% 90.5% V5 (many diffs) n/a
21 TRCN0000480164 TGTCGAGACCCGACGGGAATTTTT pLX_317 24.4% 80.2% 90.5% V5 (many diffs) n/a
22 ccsbBroadEn_13398 pDONR223 100% 23.8% 26.5% None (many diffs) n/a
23 ccsbBroad304_13398 pLX_304 0% 23.8% 26.5% V5 (many diffs) n/a
24 TRCN0000466902 ACCACAGTACACCCTTGCTTCGCA pLX_317 100% 23.8% 26.5% V5 (many diffs) n/a
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