Construct: ORF ccsbBroad304_07330

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF015039.1_s304c1, BRDN0000406693
• Derived from: ccsbBroadEn_07330
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: SGCE (8910)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_07330
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	8910	SGCE	sarcoglycan epsilon	NM_003919.3	99.8%	99.5%	146A>G;1196C>A
2	human	8910	SGCE	sarcoglycan epsilon	NM_001346717.2	97.7%	97.4%	146A>G;1037_1038ins27;1169C>A
3	human	8910	SGCE	sarcoglycan epsilon	NM_001099401.2	94.4%	94.1%	146A>G;1196C>A;1254_1328del
4	human	8910	SGCE	sarcoglycan epsilon	XM_011516665.3	92.9%	92.2%	(many diffs)
5	human	8910	SGCE	sarcoglycan epsilon	NM_001099400.2	92.8%	91.7%	(many diffs)
6	human	8910	SGCE	sarcoglycan epsilon	NM_001346713.2	92.2%	91.9%	109_216del;254A>G;1304C>A
7	human	8910	SGCE	sarcoglycan epsilon	NM_001346719.2	91.8%	91.3%	(many diffs)
8	human	8910	SGCE	sarcoglycan epsilon	XM_011516666.3	91%	90.3%	(many diffs)
9	human	8910	SGCE	sarcoglycan epsilon	NM_001301139.2	90.5%	90.3%	108_109ins123;1073C>A
10	human	8910	SGCE	sarcoglycan epsilon	NM_001346715.2	90.3%	90%	(many diffs)
11	human	8910	SGCE	sarcoglycan epsilon	NM_001362807.2	89.7%	89.2%	(many diffs)
12	human	8910	SGCE	sarcoglycan epsilon	NM_001362809.2	88.4%	88.3%	108_109ins123;914_915ins27;1046C>A
13	human	8910	SGCE	sarcoglycan epsilon	XM_011516663.2	86.2%	85.6%	(many diffs)
14	human	8910	SGCE	sarcoglycan epsilon	XM_011516667.2	85.4%	84.5%	(many diffs)
15	human	8910	SGCE	sarcoglycan epsilon	XM_017012763.1	85.4%	84.5%	(many diffs)
16	human	8910	SGCE	sarcoglycan epsilon	XM_011516664.2	84.5%	83.8%	(many diffs)
17	human	8910	SGCE	sarcoglycan epsilon	XM_024446985.1	84.2%	83.7%	(many diffs)
18	human	8910	SGCE	sarcoglycan epsilon	XM_024446986.1	82.3%	81.7%	(many diffs)
19	human	8910	SGCE	sarcoglycan epsilon	NM_001346720.2	79%	78.9%	0_1ins273;923C>A
20	human	8910	SGCE	sarcoglycan epsilon	NM_001362808.2	77%	76.8%	0_1ins273;764_765ins27;896C>A
21	human	8910	SGCE	sarcoglycan epsilon	XM_011516669.3	73.5%	73%	(many diffs)
22	human	8910	SGCE	sarcoglycan epsilon	XM_017012767.1	71.6%	71%	(many diffs)
23	mouse	20392	Sgce	sarcoglycan, epsilon	NM_011360.3	89.2%	94.5%	(many diffs)
24	mouse	20392	Sgce	sarcoglycan, epsilon	NM_001130190.1	87.1%	92.1%	(many diffs)
25	mouse	20392	Sgce	sarcoglycan, epsilon	NM_001130191.1	85.2%	90.3%	(many diffs)
26	mouse	20392	Sgce	sarcoglycan, epsilon	XM_006505023.1	84.7%	88.9%	(many diffs)
27	mouse	20392	Sgce	sarcoglycan, epsilon	NM_001130189.1	82.9%	87.1%	(many diffs)
28	mouse	20392	Sgce	sarcoglycan, epsilon	NM_001130188.1	82.4%	87.3%	(many diffs)
29	mouse	20392	Sgce	sarcoglycan, epsilon	XM_006505022.3	80.6%	85.6%	(many diffs)
30	mouse	20392	Sgce	sarcoglycan, epsilon	XM_006505021.3	80.5%	85.2%	(many diffs)
31	mouse	20392	Sgce	sarcoglycan, epsilon	XM_006505019.3	78.6%	82.4%	(many diffs)
32	mouse	20392	Sgce	sarcoglycan, epsilon	XM_006505020.3	76.9%	80.8%	(many diffs)
33	mouse	20392	Sgce	sarcoglycan, epsilon	XM_006505024.3	67%	71.3%	(many diffs)
34	mouse	20392	Sgce	sarcoglycan, epsilon	XM_011241051.2	42.5%	42.9%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1377

ORF length:

1311

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatgca attgccccgg tggtgggagc tgggagaccc ctgtgcttgg acgggacagg
121	gtcgggggac acgcaggatg agccccgcga ccactggcac attcttgctg acagtgtaca
181	gtattttctc caaggtacac tccgatcgga gtgtataccc atcagcaggt gtcctctttg
241	ttcatgtttt ggaaagagaa tattttaagg gggaatttcc accttaccca aaacctggcg
301	agattagtaa tgatcccata acatttaata caaatttaat gggttaccca gaccgacctg
361	gatggcttcg atatatccaa aggacaccat atagtgatgg agtcctatat gggtccccaa
421	cagctgaaaa tgtggggaag ccaacaatca ttgagataac tgcctacaac aggcgcacct
481	ttgagactgc aaggcataat ttgataatta atataatgtc tgcagaagac ttcccgttgc
541	catatcaagc agaattcttc attaagaata tgaatgtaga agaaatgttg gccagtgagg
601	ttcttggaga ctttcttggc gcagtgaaaa atgtgtggca gccagagcgc ctgaacgcca
661	taaacatcac atcggcccta gacaggggtg gcagggtgcc acttcccatt aatgacctga
721	aggagggcgt ttatgtcatg gttggtgcag atgtcccgtt ttcttcttgt ttacgagaag
781	ttgaaaatcc acagaatcaa ttgagatgta gtcaagaaat ggagcctgta ataacatgtg
841	ataaaaaatt tcgtactcaa ttttacattg actggtgcaa aatttcattg gttgataaaa
901	caaagcaagt gtccacctat caggaagtga ttcgtggaga ggggatttta cctgatggtg
961	gagaatacaa acccccttct gattctttga aaagcagaga ctattacacg gatttcctaa
1021	ttacactggc tgtgccctcg gcagtggcac tggtcctttt tctaatactt gcttatatca
1081	tgtgctgccg acgggaaggc gtggaaaaga gaaacatgca aacaccagac atccaactgg
1141	tccatcacag tgctattcag aaatctacca aggagcttcg agacatgtcc aagaatagag
1201	agatagcatg gcccctgtca acgcttcctg tgttccaccc tgtgactggg gaaatcatac
1261	atcctttaca cacagacaac tatgatagca caaacatgcc attgatgcaa acgcagcaga
1321	acttgccaca tcagactcag attccccaac agcagactac aggtaaatgg tatccctgcc
1381	caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1441	ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
1501	tta

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