Construct: ORF ccsbBroad304_12372
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018080.1_s304c1, BRDN0000401514
- Derived from:
- ccsbBroadEn_12372
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDZD4 (57595)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12372
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57595 | PDZD4 | PDZ domain containing 4 | NM_001303512.1 | 100% | 100% | |
| 2 | human | 57595 | PDZD4 | PDZ domain containing 4 | NM_032512.4 | 99.2% | 99.2% | 296_297insGTACGTCCTCTCTGAGCT |
| 3 | human | 57595 | PDZD4 | PDZ domain containing 4 | NM_001303515.1 | 89.5% | 89.5% | 0_1ins243 |
| 4 | human | 57595 | PDZD4 | PDZ domain containing 4 | NM_001303516.1 | 88.7% | 88.7% | 0_1ins243;53_54insGTACGTCCTCTCTGAGCT |
| 5 | human | 57595 | PDZD4 | PDZ domain containing 4 | NM_001303513.2 | 86.6% | 86.4% | 1_1delAins308;5_6delCC;8_9insT |
| 6 | human | 57595 | PDZD4 | PDZ domain containing 4 | NM_001303514.1 | 85.1% | 85.1% | 58_59ins345 |
| 7 | mouse | 245469 | Pdzd4 | PDZ domain containing 4 | NM_001029868.2 | 86.5% | 93% | (many diffs) |
| 8 | mouse | 245469 | Pdzd4 | PDZ domain containing 4 | XM_006528017.3 | 83.9% | 90.3% | (many diffs) |
| 9 | mouse | 245469 | Pdzd4 | PDZ domain containing 4 | NM_001290540.1 | 72.7% | 78.8% | (many diffs) |
| 10 | mouse | 245469 | Pdzd4 | PDZ domain containing 4 | XM_006528020.2 | 72.2% | 78.1% | (many diffs) |
| 11 | mouse | 245469 | Pdzd4 | PDZ domain containing 4 | XM_006528021.3 | 68.3% | 75% | (many diffs) |
| 12 | mouse | 245469 | Pdzd4 | PDZ domain containing 4 | XM_006528022.3 | 66% | 72.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2394
- ORF length:
- 2325
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggatgtaat atgtgcgtgg tccagaaacc ggaggagcag tacaaagtga 121 tgctgcaggt gaacgggaag gagctctcca agctgtctca ggagcaaact ctgcaggccc 181 tgcgctcctc caaggagccc ctggtgatcc aggtgctgag acgcagcccc cgcctccggg 241 gggacagctc ctgtcacgac ctgcagctgg tggacagtgg cactcagacc gacatcacct 301 tcgagcatat catggcgctg ggcaagctgc gtccgcccac cccgcccatg gtcatcctgg 361 agccgtacgt cctctctgag ctccccccaa tcagccatga gtattatgac ccggcggagt 421 ttatggaggg cggcccgcag gaggcagacc gcttggatga gctggagtat gaggaggtgg 481 agctgtataa aagcagccac cgggacaagc tgggcctgat ggtttgctac cgcacggacg 541 acgaggagga cctgggcatt tatgtcggag aggtaaatcc caacagcatt gcagccaaag 601 acggccggat ccgtgaggga gaccgcatca tccagattaa cggtgtagac gtccagaacc 661 gggaagaggc ggtggccatc ctgagccagg aagagaacac caacatctcc ctgctggtgg 721 cccgacctga gagtcagctg gcgaaaaggt ggaaggacag cgaccgggat gacttcctgg 781 atgactttgg ctctgagaat gagggggagc tgcgtgctcg taaactgaaa tcaccccctg 841 cccagcagcc cggaaacgaa gaggagaagg gggctcccga tgccggccca ggcctgagca 901 acagccagga gctggacagc ggggtgggcc ggactgacga gagcacccgg aacgaagaga 961 gctctgagca cgacctgctg ggggacgaac ccccgagctc caccaacacc ccgggaagcc 1021 tgcgcaagtt tggcctgcaa ggggacgccc tgcagagccg ggacttccat ttcagcatgg 1081 actctctgct ggccgagggg gcggggctgg gagggggcga cgtcccgggc ctcacggatg 1141 aggagtatga gcgctaccgt gagctcctgg agatcaagtg ccacctggag aacggcaacc 1201 agctgggcct cctctttccc cgggcctccg gaggcaacag cgccctggac gtcaaccgca 1261 acgagagcct gggccacgag atggccatgc tggaggagga gctaaggcac ctggaattca 1321 agtgccgcaa catactgcgg gcgcagaaga tgcagcagct gcgtgagcgc tgcatgaagg 1381 cctggctgct ggaggaggag agcctctacg acctggcggc cagcgagccc aagaagcacg 1441 agctgtccga catctccgag ctgcccgaga agtcggacaa ggacagcacc agcgcctaca 1501 acactgggga gagctgccgc agcaccccgc tgcttgtgga gcccctgccc gagagccccc 1561 tgcggcgggc catggccggc aactccaact tgaaccggac ccctcccggc cccgctgttg 1621 ccacccccgc caaggcagct cctccaccgg ggagccccgc caagttccgg tccctctccc 1681 gggatcctga ggccggccgg aggcagcacg cggaggagcg cggccgccgc aaccccaaga 1741 cggggttgac cctggagcgt gtgggccctg aaagcagccc ttacctctcg cggcgccacc 1801 gcggccaggg ccaggagggc gagcactacc acagctgcgt gcagctggcc ccgacgcgag 1861 gcctggagga gctgggccac ggccccctga gcttggccgg tggccctcgg gtgggcgggg 1921 tggcggccgc ggccactgaa gcaccgcgca tggagtggaa agtgaaggtg cgcagcgacg 1981 gaacccgcta cgtggccaag cggcccgtgc gagatcggct gctgaaagcc cgtgccctga 2041 agatccggga ggagcgcagc ggtatgacga ccgacgacga cgcggtgagc gagatgaaga 2101 tgggccgcta ctggagcaag gaggagcgga agcagcacct gatccgggcc cgtgagcagc 2161 ggaagcggcg cgagttcatg atgcagagcc ggctggagtg cctgcgggag cagcagaatg 2221 gcgacagcaa gcccgagctc aacatcattg ccctgagcca ccgcaaaacc atgaagaagc 2281 ggaacaagaa gatcctggac aactggatca ccatccagga gatgctggcc cacggcgcgc 2341 gctccgccga tggcaagcgg gtctacaacc ctcttctctc agtcaccacc gtgttgccaa 2401 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2461 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta