Construct: ORF ccsbBroad304_14752

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018962.1_s304c1, BRDN0000459446
Derived from:
ccsbBroadEn_14752
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PHKA2 (5256)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14752
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5256 PHKA2 phosphorylase kinase regula... NM_000292.3 99.4% 19.7% (many diffs)
2 human 5256 PHKA2 phosphorylase kinase regula... XM_006724496.4 98.7% 19.6% (many diffs)
3 human 5256 PHKA2 phosphorylase kinase regula... XM_005274548.5 97.9% 20% (many diffs)
4 human 5256 PHKA2 phosphorylase kinase regula... XM_005274550.5 97.1% 20.2% (many diffs)
5 human 5256 PHKA2 phosphorylase kinase regula... XM_011545537.3 96.1% 17.4% (many diffs)
6 human 5256 PHKA2 phosphorylase kinase regula... XM_006724498.4 84.1% 4.9% (many diffs)
7 human 5256 PHKA2 phosphorylase kinase regula... XR_002958777.1 83.5% (many diffs)
8 human 5256 PHKA2 phosphorylase kinase regula... XR_001755697.2 81.6% (many diffs)
9 human 5256 PHKA2 phosphorylase kinase regula... XM_017029580.2 74.5% 5.6% (many diffs)
10 human 5256 PHKA2 phosphorylase kinase regula... XR_950461.3 72.1% (many diffs)
11 human 5256 PHKA2 phosphorylase kinase regula... XM_011545538.3 71.3% 5.9% (many diffs)
12 human 5256 PHKA2 phosphorylase kinase regula... XR_001755698.2 52.4% (many diffs)
13 mouse 110094 Phka2 phosphorylase kinase alpha 2 NM_001177879.1 86.8% 19.4% (many diffs)
14 mouse 110094 Phka2 phosphorylase kinase alpha 2 NM_172783.3 86.8% 19.4% (many diffs)
15 mouse 110094 Phka2 phosphorylase kinase alpha 2 XM_006528679.3 86.4% 19.3% (many diffs)
16 mouse 110094 Phka2 phosphorylase kinase alpha 2 XM_006528680.3 84.8% 19.7% (many diffs)
17 mouse 110094 Phka2 phosphorylase kinase alpha 2 NM_001177878.1 83.4% 20.1% (many diffs)
18 mouse 110094 Phka2 phosphorylase kinase alpha 2 XM_006528681.1 82.6% 14.8% (many diffs)
19 mouse 110094 Phka2 phosphorylase kinase alpha 2 XR_387078.3 75.9% (many diffs)
20 mouse 110094 Phka2 phosphorylase kinase alpha 2 XM_006528682.1 73.5% 4.9% (many diffs)
21 mouse 110094 Phka2 phosphorylase kinase alpha 2 XR_001782730.1 70.5% (many diffs)
22 mouse 110094 Phka2 phosphorylase kinase alpha 2 XR_001782731.1 40.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
828
ORF length:
759
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcggagcagg agcaattccg gggtccgctt ggacgggtac gcgcggctgg
121tgcagcaaac catcctgtgt taccagaatc ccgtcacggg gctgctgtca gccagccatg
181agcagaagga tgcctgggtg cgggataaca tctacagtat cctggccgtg tggggcctgg
241gcatggccta ccgtaagaat gcagaccgcg atgaggacaa ggccaaggcc tacgagctgg
301agcagaacgt ggtgaagctg atgcgaggtc ttctccagtg catgatgaga caggtggcca
361aagtggagaa gttcaaacac actcagagca ccaaggacag cctgcacgcc aagtacaaca
421ccgccacctg tggcacggtg gtgggcgacg accagtgggg ccacctccag gtggatgcca
481cctctctctt cctcctgttc ctggcccaga tgaccgcctc aggcttacgt atcattttca
541ctctcgatga ggtggccttc atacagaatc ttgtctttta catagaagct gcatataaag
601tcgctgatta tggaatgtgg gagcgtggag ataagactaa tcagggcatc ccggaattga
661atgcaagctc cgtaggaatg gccaaggcag ctcttgaggc aattgatgaa ctggaccttt
721ttggagccca tggaggacgc aagtcagtga ttcatgttct gccagatgag gtcgagcact
781gccagtctta ttcttgttct ccatgctgcc caagaagcgt cgacatctaa agaaattgat
841gctggacttc tttccattat ttccttcccg gcctttgcag tggaagatgt aaaccttgta
901aatgtgacca aaaatgaaat tatttctaag ctccaggggc gttatggatg ctgtcgcttc
961cttcgagatg gttataaaac tccaagagag gaccctaatc gactgcatta tgaccctgct
1021gaactcaagc tcttcgaaaa cattgaatgt gagtggcctg tgttttggac atattttata
1081atagatggag tcttcagtgg tgatgctgtt caggtccaag aataccgaga ggccctggag
1141ggaatactca tcagaggcaa gaatgggatc cgcctggtgc ctgaactcta cgctgtcccg
1201cctaacaagg tagatgaaga gtacaagaat cctcacacag tagaccgagt tcctatgggg
1261aaggtgcctc atctgtgggg ccaatccttg tacatcctca gctcgctgtt ggcagaggga
1321ttccttgccg ctggtgaaat cgatccctta aatagaagat tttccacttc agtcaaacct
1381gatgttgtag tacaagttac tgttttggca gaaaacaatc acattaagga cttattgagg
1441aaacacgggg tgaacgtcca gagtatcgcg gacattcatc caattcaagt ccagccgggc
1501cggattctta gtcacatata tgccaagctt ggacggaata agaatatgaa tttgagtggg
1561cgaccgtatc gacatattgg tgtccttgga acctctaaac tatatgtgat taggaaccaa
1621atctttactt ttacacccca gttcaccgac gagcatcact tctacctggc cctcgacaat
1681gagatgatcg tggagatgct aaggatcgag ctggcctacc tgtgcacctg ctggaggatg
1741acgggcagac ccacactcac cttccccatc agtcgcacca tgctcacaaa tgatggctca
1801gacattcatt ctgctgtgct ctccacaatt agaaaactag aggatggata ttttggagga
1861gccagagtaa aattagggaa cctttcggaa tttctcacca catcgttcta cacatatctg
1921acttttctgg atccagactg tgatgagaag ttgtttgaca atgccagcga agggactttc
1981agtcctgata gtgattcaga tttggtagga tatctggaag acacctgtaa tcaagaaagc
2041caagacgaac ttgaccatta tatcaaccac cttctgcaaa gcacatcgtt gaggtcctat
2101ctgcctcctc tttgtaagaa cacagaagac cgccatgtct tcagtgctat ccactccacg
2161cgggacatac tttctgtgat ggcaaaagca aagggtttgg aagttccatt tgttcccatg
2221actttgccga ctaaagttct aagtgcccac cgtaaatcac tgaatcttgt tgattctcct
2281cagccactcc tagaaaaggt tcctgaaagt gactttcagt ggcccagaga tgaccatagt
2341gacgtggact gtgagaagct ggttgagcag ctaaaagatt gttcgaacct acaggaccaa
2401gcagacattc tgtacattct ttatgtcata aagggtccca gctgggacac aaatctctct
2461ggacagcacg gggtcaccgt tcaaaacctt cttggtgagc tctatgggaa agccggcttg
2521aaccaggagt ggggtctgat tcgctacatc tcaggccttc tcaggaagaa agtggaggtc
2581ctggctgagg cctgcacaga cctgctttcg caccagaagc agctcaccgt gggcctgccg
2641cccgagcccc gggagaagat catctctgcg ccccttcccc cagaggagct cacaaaactc
2701atctacgagg ccagtgggca ggacatcagc attgccgtcc tcacgcagga gattgtggtt
2761tacctggcca tgtatgtcag ggcgcagccc agcctctttg tggagatgct gagactccgg
2821attggactga tcattcaggt gatggccacg gagctggcac ggagcctgaa ctgctcagga
2881gaagaggctt ctgaaagttt gatgaacctc agccctttcg atatgaaaaa tctcctgcac
2941catattctaa gtgggaaaga gtttggcgtt gaaagaagtg tgcgccctat ccactctcac
3001aatcagccct acatctcatc acgaggtgtc atacggagtc acaaaactga gaggagtggc
3061attacagact gagagtgaaa tgaaacagat gactaggcgg tttagtgctg atgaacagtt
3121cttttctgtg ggccaggccg cgtccagcag tgcgcattcc tccaagtctg cgaggtccag
3181caccccatcc tcgcccactg gcacgtcatc ctcagactcg ggaggacatc acatcggctg
3241gggtgagcgg cagggccagt ggctgcgcag gagaaggctg gatggggcca tcaacagggt
3301ccccgtggga ttctaccaga gggtgtggaa gatcctccag aagtgccacg gtctctccat
3361cgatggttat gtcctcccat cctcgacgac ccgagagatg accccgcatg agatcaagtt
3421tgctgtccat gtcgaatcgg tgctgaaccg cgtgccgcag cccgagtacc ggcagctgct
3481ggtggaagcc atcatggtgc tgacgctgct ctcggacacg gagatgacca gcatcggggg
3541catcatccac gtggaccaga tcgtgcagat ggccagtcag ctgttcttgc aggaccaggt
3601gtcaattggt gccatggaca ccctggagaa agaccaagcc acaggaatct gccacttctt
3661ttatgacagc gctccgagtg gggcttatgg gacgatgacc tacctaacaa gagcagtggc
3721ttcttatttg caggaattgt tgcccaattc gggctgccag atgcaattgc caactttctt
3781gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta
3841atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga tta

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