Construct: ORF ccsbBroad304_14752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018962.1_s304c1, BRDN0000459446
- Derived from:
- ccsbBroadEn_14752
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PHKA2 (5256)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14752
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5256 | PHKA2 | phosphorylase kinase regula... | NM_000292.3 | 99.4% | 19.7% | (many diffs) |
| 2 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_006724496.4 | 98.7% | 19.6% | (many diffs) |
| 3 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_005274548.5 | 97.9% | 20% | (many diffs) |
| 4 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_005274550.5 | 97.1% | 20.2% | (many diffs) |
| 5 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_011545537.3 | 96.1% | 17.4% | (many diffs) |
| 6 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_006724498.4 | 84.1% | 4.9% | (many diffs) |
| 7 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XR_002958777.1 | 83.5% | (many diffs) | |
| 8 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XR_001755697.2 | 81.6% | (many diffs) | |
| 9 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_017029580.2 | 74.5% | 5.6% | (many diffs) |
| 10 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XR_950461.3 | 72.1% | (many diffs) | |
| 11 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XM_011545538.3 | 71.3% | 5.9% | (many diffs) |
| 12 | human | 5256 | PHKA2 | phosphorylase kinase regula... | XR_001755698.2 | 52.4% | (many diffs) | |
| 13 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | NM_001177879.1 | 86.8% | 19.4% | (many diffs) |
| 14 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | NM_172783.3 | 86.8% | 19.4% | (many diffs) |
| 15 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XM_006528679.3 | 86.4% | 19.3% | (many diffs) |
| 16 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XM_006528680.3 | 84.8% | 19.7% | (many diffs) |
| 17 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | NM_001177878.1 | 83.4% | 20.1% | (many diffs) |
| 18 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XM_006528681.1 | 82.6% | 14.8% | (many diffs) |
| 19 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XR_387078.3 | 75.9% | (many diffs) | |
| 20 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XM_006528682.1 | 73.5% | 4.9% | (many diffs) |
| 21 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XR_001782730.1 | 70.5% | (many diffs) | |
| 22 | mouse | 110094 | Phka2 | phosphorylase kinase alpha 2 | XR_001782731.1 | 40.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 828
- ORF length:
- 759
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggagcagg agcaattccg gggtccgctt ggacgggtac gcgcggctgg 121 tgcagcaaac catcctgtgt taccagaatc ccgtcacggg gctgctgtca gccagccatg 181 agcagaagga tgcctgggtg cgggataaca tctacagtat cctggccgtg tggggcctgg 241 gcatggccta ccgtaagaat gcagaccgcg atgaggacaa ggccaaggcc tacgagctgg 301 agcagaacgt ggtgaagctg atgcgaggtc ttctccagtg catgatgaga caggtggcca 361 aagtggagaa gttcaaacac actcagagca ccaaggacag cctgcacgcc aagtacaaca 421 ccgccacctg tggcacggtg gtgggcgacg accagtgggg ccacctccag gtggatgcca 481 cctctctctt cctcctgttc ctggcccaga tgaccgcctc aggcttacgt atcattttca 541 ctctcgatga ggtggccttc atacagaatc ttgtctttta catagaagct gcatataaag 601 tcgctgatta tggaatgtgg gagcgtggag ataagactaa tcagggcatc ccggaattga 661 atgcaagctc cgtaggaatg gccaaggcag ctcttgaggc aattgatgaa ctggaccttt 721 ttggagccca tggaggacgc aagtcagtga ttcatgttct gccagatgag gtcgagcact 781 gccagtctta ttcttgttct ccatgctgcc caagaagcgt cgacatctaa agaaattgat 841 gctggacttc tttccattat ttccttcccg gcctttgcag tggaagatgt aaaccttgta 901 aatgtgacca aaaatgaaat tatttctaag ctccaggggc gttatggatg ctgtcgcttc 961 cttcgagatg gttataaaac tccaagagag gaccctaatc gactgcatta tgaccctgct 1021 gaactcaagc tcttcgaaaa cattgaatgt gagtggcctg tgttttggac atattttata 1081 atagatggag tcttcagtgg tgatgctgtt caggtccaag aataccgaga ggccctggag 1141 ggaatactca tcagaggcaa gaatgggatc cgcctggtgc ctgaactcta cgctgtcccg 1201 cctaacaagg tagatgaaga gtacaagaat cctcacacag tagaccgagt tcctatgggg 1261 aaggtgcctc atctgtgggg ccaatccttg tacatcctca gctcgctgtt ggcagaggga 1321 ttccttgccg ctggtgaaat cgatccctta aatagaagat tttccacttc agtcaaacct 1381 gatgttgtag tacaagttac tgttttggca gaaaacaatc acattaagga cttattgagg 1441 aaacacgggg tgaacgtcca gagtatcgcg gacattcatc caattcaagt ccagccgggc 1501 cggattctta gtcacatata tgccaagctt ggacggaata agaatatgaa tttgagtggg 1561 cgaccgtatc gacatattgg tgtccttgga acctctaaac tatatgtgat taggaaccaa 1621 atctttactt ttacacccca gttcaccgac gagcatcact tctacctggc cctcgacaat 1681 gagatgatcg tggagatgct aaggatcgag ctggcctacc tgtgcacctg ctggaggatg 1741 acgggcagac ccacactcac cttccccatc agtcgcacca tgctcacaaa tgatggctca 1801 gacattcatt ctgctgtgct ctccacaatt agaaaactag aggatggata ttttggagga 1861 gccagagtaa aattagggaa cctttcggaa tttctcacca catcgttcta cacatatctg 1921 acttttctgg atccagactg tgatgagaag ttgtttgaca atgccagcga agggactttc 1981 agtcctgata gtgattcaga tttggtagga tatctggaag acacctgtaa tcaagaaagc 2041 caagacgaac ttgaccatta tatcaaccac cttctgcaaa gcacatcgtt gaggtcctat 2101 ctgcctcctc tttgtaagaa cacagaagac cgccatgtct tcagtgctat ccactccacg 2161 cgggacatac tttctgtgat ggcaaaagca aagggtttgg aagttccatt tgttcccatg 2221 actttgccga ctaaagttct aagtgcccac cgtaaatcac tgaatcttgt tgattctcct 2281 cagccactcc tagaaaaggt tcctgaaagt gactttcagt ggcccagaga tgaccatagt 2341 gacgtggact gtgagaagct ggttgagcag ctaaaagatt gttcgaacct acaggaccaa 2401 gcagacattc tgtacattct ttatgtcata aagggtccca gctgggacac aaatctctct 2461 ggacagcacg gggtcaccgt tcaaaacctt cttggtgagc tctatgggaa agccggcttg 2521 aaccaggagt ggggtctgat tcgctacatc tcaggccttc tcaggaagaa agtggaggtc 2581 ctggctgagg cctgcacaga cctgctttcg caccagaagc agctcaccgt gggcctgccg 2641 cccgagcccc gggagaagat catctctgcg ccccttcccc cagaggagct cacaaaactc 2701 atctacgagg ccagtgggca ggacatcagc attgccgtcc tcacgcagga gattgtggtt 2761 tacctggcca tgtatgtcag ggcgcagccc agcctctttg tggagatgct gagactccgg 2821 attggactga tcattcaggt gatggccacg gagctggcac ggagcctgaa ctgctcagga 2881 gaagaggctt ctgaaagttt gatgaacctc agccctttcg atatgaaaaa tctcctgcac 2941 catattctaa gtgggaaaga gtttggcgtt gaaagaagtg tgcgccctat ccactctcac 3001 aatcagccct acatctcatc acgaggtgtc atacggagtc acaaaactga gaggagtggc 3061 attacagact gagagtgaaa tgaaacagat gactaggcgg tttagtgctg atgaacagtt 3121 cttttctgtg ggccaggccg cgtccagcag tgcgcattcc tccaagtctg cgaggtccag 3181 caccccatcc tcgcccactg gcacgtcatc ctcagactcg ggaggacatc acatcggctg 3241 gggtgagcgg cagggccagt ggctgcgcag gagaaggctg gatggggcca tcaacagggt 3301 ccccgtggga ttctaccaga gggtgtggaa gatcctccag aagtgccacg gtctctccat 3361 cgatggttat gtcctcccat cctcgacgac ccgagagatg accccgcatg agatcaagtt 3421 tgctgtccat gtcgaatcgg tgctgaaccg cgtgccgcag cccgagtacc ggcagctgct 3481 ggtggaagcc atcatggtgc tgacgctgct ctcggacacg gagatgacca gcatcggggg 3541 catcatccac gtggaccaga tcgtgcagat ggccagtcag ctgttcttgc aggaccaggt 3601 gtcaattggt gccatggaca ccctggagaa agaccaagcc acaggaatct gccacttctt 3661 ttatgacagc gctccgagtg gggcttatgg gacgatgacc tacctaacaa gagcagtggc 3721 ttcttatttg caggaattgt tgcccaattc gggctgccag atgcaattgc caactttctt 3781 gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta 3841 atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga tta