Construct: ORF ccsbBroadEn_00024
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001035.1_s300c1, BRDN0000395067
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADCY3 (109)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 109 | ADCY3 | adenylate cyclase 3 | NM_004036.5 | 100% | 100% | |
| 2 | human | 109 | ADCY3 | adenylate cyclase 3 | NM_001320613.2 | 99.9% | 99.9% | 2493_2495delCAG |
| 3 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_017003187.1 | 98.3% | 98.3% | 1660_1716del |
| 4 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_011532491.1 | 98.2% | 98.2% | 1660_1716del;2550_2552delCAG |
| 5 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_017003186.1 | 98.1% | 98.1% | 1356_1421del |
| 6 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_006711925.1 | 98% | 98% | 1356_1421del;2559_2561delCAG |
| 7 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_011532490.1 | 96.5% | 96.5% | 1356_1421del;1726_1782del |
| 8 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_011532489.1 | 96.4% | 96.4% | 1356_1421del;1726_1782del;2616_2618delCAG |
| 9 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_017003190.1 | 95.9% | 95.9% | 2171_2172ins138 |
| 10 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_011532494.1 | 94.1% | 94.1% | 1356_1421del;2237_2238ins138 |
| 11 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_017003188.1 | 92.6% | 90.8% | (many diffs) |
| 12 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_017003189.1 | 92.6% | 92.6% | 1356_1421del;1726_1782del;2294_2295ins138 |
| 13 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_011532492.2 | 92.6% | 90.7% | (many diffs) |
| 14 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_011532493.1 | 92.5% | 92.5% | (many diffs) |
| 15 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_017003193.1 | 78.9% | 78.9% | 0_1ins723 |
| 16 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_017003192.1 | 78.8% | 78.8% | 0_1ins723;1770_1772delCAG |
| 17 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_017003191.1 | 78.1% | 77.8% | (many diffs) |
| 18 | human | 109 | ADCY3 | adenylate cyclase 3 | XM_011532496.1 | 76.1% | 76.1% | (many diffs) |
| 19 | mouse | 104111 | Adcy3 | adenylate cyclase 3 | NM_001159536.1 | 89.8% | 94.4% | (many diffs) |
| 20 | mouse | 104111 | Adcy3 | adenylate cyclase 3 | NM_001159537.1 | 89.8% | 94.4% | (many diffs) |
| 21 | mouse | 104111 | Adcy3 | adenylate cyclase 3 | NM_138305.3 | 89.7% | 94.4% | (many diffs) |
| 22 | mouse | 104111 | Adcy3 | adenylate cyclase 3 | XM_006514932.2 | 89.7% | 94.4% | (many diffs) |
| 23 | mouse | 104111 | Adcy3 | adenylate cyclase 3 | XM_006514933.2 | 89.7% | 94.4% | (many diffs) |
| 24 | mouse | 104111 | Adcy3 | adenylate cyclase 3 | XM_006514934.3 | 89.7% | 94.4% | (many diffs) |
| 25 | mouse | 104111 | Adcy3 | adenylate cyclase 3 | XM_006514935.3 | 89.7% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3501
- ORF length:
- 3432
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GCCGAGGAAC CAGGGCTTCT CCGAGCCCGA ATACTCGGCC GAGTACTCAG 121 CCGAGTACTC CGTCAGCCTG CCCTCCGACC CTGACCGCGG GGTGGGCCGG ACCCATGAAA 181 TCTCGGTCCG GAACTCGGGC TCCTGCCTGT GCCTGCCTCG CTTCATGCGG CTGACTTTCG 241 TGCCGGAGTC CTTGGAGAAC CTCTACCAGA CCTACTTCAA AAGGCAGCGC CACGAGACCC 301 TGCTGGTGCT GGTGGTCTTT GCAGCCCTCT TTGACTGCTA CGTGGTGGTC ATGTGTGCTG 361 TGGTCTTCTC CAGCGACAAG CTGGCTTCCC TCGCCGTGGC TGGAATTGGA CTGGTGTTGG 421 ACATCATCCT CTTCGTGCTC TGCAAAAAGG GGCTGCTCCC GGACCGGGTC ACCCGCAGAG 481 TGCTGCCCTA CGTGCTGTGG CTGCTCATAA CCGCCCAGAT CTTCTCCTAC CTGGGCCTGA 541 ACTTCGCGCG TGCCCACGCG GCTAGTGACA CGGTGGGCTG GCAGGTCTTC TTTGTCTTCT 601 CCTTCTTCAT CACGCTGCCC CTCAGCCTCA GCCCCATCGT GATCATCTCC GTGGTCTCCT 661 GTGTGGTGCA CACGTTGGTC CTGGGGGTCA CCGTGGCCCA GCAGCAGCAG GAGGAGCTCA 721 AGGGGATGCA GCTGCTGCGG GAGATCCTGG CCAACGTCTT CCTCTACCTG TGCGCCATCG 781 CTGTGGGCAT CATGTCCTAC TACATGGCTG ACCGCAAGCA CCGCAAGGCC TTCCTGGAGG 841 CCCGCCAGTC GCTGGAGGTG AAGATGAACC TGGAAGAGCA GAGCCAGCAG CAGGAGAACC 901 TCATGCTTTC CATCCTGCCC AAGCACGTGG CTGACGAGAT GCTGAAAGAC ATGAAGAAAG 961 ACGAGAGCCA GAAGGACCAG CAGCAGTTCA ACACCATGTA CATGTACCGT CACGAGAACG 1021 TCAGCATCCT CTTTGCCGAC ATCGTGGGCT TTACCCAGCT GTCTTCTGCC TGCAGTGCCC 1081 AGGAGCTTGT GAAGCTGCTC AACGAGCTCT TTGCCCGCTT TGACAAGCTG GCAGCTAAAT 1141 ACCACCAGCT GCGGATTAAG ATCCTGGGCG ACTGCTACTA CTGCATCTGC GGCTTGCCCG 1201 ACTACCGGGA GGACCACGCC GTCTGCTCCA TCCTCATGGG GCTGGCCATG GTGGAGGCCA 1261 TCTCGTATGT GCGGGAGAAG ACCAAGACTG GGGTGGACAT GCGTGTGGGG GTGCACACGG 1321 GCACCGTGCT GGGGGGCGTC CTGGGCCAGA AGCGCTGGCA GTACGACGTG TGGTCGACTG 1381 ATGTCACTGT AGCCAACAAG ATGGAGGCCG GCGGCATCCC TGGGCGCGTG CACATCTCCC 1441 AGAGCACCAT GGACTGCCTG AAAGGGGAGT TTGATGTGGA GCCAGGCGAT GGGGGCAGCC 1501 GCTGTGATTA CCTAGAAGAG AAGGGTATTG AAACCTACCT CATCATTGCC TCCAAGCCAG 1561 AGGTGAAGAA AACAGCCACC CAGAATGGCC TCAATGGCTC GGCCCTGCCC AATGGAGCAC 1621 CAGCTTCCTC AAAGTCCAGC TCCCCTGCCC TCATTGAGAC CAAGGAGCCC AACGGGAGTG 1681 CCCACAGCAG TGGGTCCACG TCGGAGAAGC CCGAGGAGCA GGATGCCCAG GCCGACAACC 1741 CCTCATTCCC CAACCCACGC CGGAGGCTGC GCCTGCAGGA CCTGGCTGAC CGAGTGGTGG 1801 ATGCCTCTGA AGATGAGCAC GAGCTCAACC AGCTGCTCAA CGAGGCCCTG CTTGAGCGAG 1861 AGTCCGCCCA AGTAGTAAAG AAGAGAAACA CCTTCCTCTT GTCCATGCGG TTCATGGACC 1921 CCGAGATGGA AACCCGCTAC TCGGTGGAGA AGGAGAAGCA GAGTGGGGCT GCCTTCAGCT 1981 GCTCCTGCGT CGTCCTGCTC TGCACGGCCC TGGTCGAGAT ACTCATCGAC CCCTGGCTAA 2041 TGACAAACTA TGTGACCTTC ATGGTGGGGG AGATTCTGCT CCTCATCCTG ACCATCTGCT 2101 CCCTGGCTGC CATCTTTCCC CGGGCCTTTC CTAAGAAGCT TGTGGCCTTC TCAACTTGGA 2161 TTGACCGGAC CCGCTGGGCC AGGAACACCT GGGCCATGCT CGCCATCTTC ATCCTGGTGA 2221 TGGCAAATGT CGTGGACATG CTCAGCTGTC TCCAGTACTA CACGGGACCC AGCAATGCAA 2281 CGGCAGGGAT GGAAACGGAG GGCAGCTGCC TGGAGAACCC CAAGTATTAC AACTATGTGG 2341 CCGTGCTGTC CCTCATCGCC ACCATCATGC TGGTGCAGGT CAGCCACATG GTGAAGCTCA 2401 CGCTCATGCT GCTCGTCGCA GGCGCCGTGG CCACCATCAA CCTCTATGCC TGGCGTCCCG 2461 TCTTTGATGA ATACGACCAC AAGCGTTTTC GGGAGCACGA CTTACCTATG GTGGCCTTAG 2521 AGCAGATGCA AGGATTCAAC CCTGGGCTCA ATGGCACTGA CAGGCTGCCC CTGGTGCCTT 2581 CCAAGTACTC TATGACGGTG ATGGTGTTCC TCATGATGCT CAGCTTCTAC TACTTCTCCC 2641 GCCACGTAGA AAAACTGGCA CGGACACTTT TCTTGTGGAA GATTGAGGTC CACGACCAGA 2701 AGGAACGTGT CTATGAGATG CGACGCTGGA ACGAGGCCTT GGTCACCAAC ATGTTGCCTG 2761 AGCACGTGGC ACGCCATTTC CTGGGGTCCA AGAAGAGAGA TGAGGAGCTG TATAGCCAGA 2821 CGTATGATGA GATTGGAGTC ATGTTTGCCT CCCTGCCCAA CTTTGCTGAC TTCTACACAG 2881 AGGAGAGCAT CAACAATGGT GGTATTGAGT GTCTGCGTTT CCTCAATGAA ATCATCTCAG 2941 ATTTTGACTC TCTCCTGGAC AATCCCAAGT TCCGGGTGAT CACCAAGATC AAAACCATTG 3001 GCAGCACGTA TATGGCGGCT TCAGGAGTCA CCCCCGATGT CAACACCAAT GGCTTTGCCA 3061 GCTCCAACAA GGAAGACAAG TCCGAGAGAG AGCGCTGGCA GCACCTGGCT GACCTGGCCG 3121 ACTTCGCGCT GGCCATGAAG GATACGCTCA CCAACATCAA CAACCAGTCC TTCAATAACT 3181 TCATGCTGCG CATAGGCATG AACAAAGGCG GGGTTCTGGC TGGGGTCATC GGAGCCCGGA 3241 AACCACACTA CGACATCTGG GGCAATACAG TCAATGTAGC CAGCAGGATG GAGTCCACGG 3301 GGGTCATGGG CAACATTCAG GTGGTAGAAG AAACCCAAGT CATCCTCCGA GAGTACGGCT 3361 TCCGCTTTGT GAGGCGAGGC CCCATCTTTG TGAAGGGGAA GGGGGAGCTG CTGACCTTCT 3421 TCTTGAAGGG GCGGGATAAG CTAGCCACCT TCCCCAATGG CCCCTCTGTC ACACTGCCCC 3481 ACCAGGTGGT GGACAACTCC TTGCCAACTT TTCTTGTACA AAGTtggcat tataagaaag 3541 cattgcttat caatttgttg caacgaac