Construct: ORF ccsbBroadEn_10968

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003521.1_s300c1, BRDN0000395246
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MIA2 (4253)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001247990.2 100% 100%
2 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354140.2 99.3% 99.3% 143_144insGGTAGAAAATCAAAT
3 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354141.2 99.3% 99.3% 143_144insGGTAGAAAATCAAAT
4 human 4253 MIA2 MIA SH3 domain ER export fa... XM_011536785.2 99.3% 99.3% 143_144insGGTAGAAAATCAAAT
5 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021323.1 98.7% 98.7% 159_185del
6 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021324.2 98.7% 98.7% 159_185del
7 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449598.1 98.7% 98.7% 159_185del
8 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354156.2 95.7% 95.7% 65_66ins93
9 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354142.1 93.4% 93.4% 143_144insGGTAGAAAATCAAAT;1299_1300ins129
10 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354143.1 93.4% 93.4% 143_144insGGTAGAAAATCAAAT;1299_1300ins129
11 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354144.2 93.4% 93.4% 143_144insGGTAGAAAATCAAAT;1299_1300ins129
12 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021327.1 93.4% 93.4% 143_144insGGTAGAAAATCAAAT;1299_1300ins129
13 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354137.1 92.8% 92.8% 1_153del;296_297insGGTAGAAAATCAAAT
14 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354138.1 92.8% 92.8% 1_153del;296_297insGGTAGAAAATCAAAT
15 human 4253 MIA2 MIA SH3 domain ER export fa... NM_203356.2 92.8% 92.8% 1_153del;296_297insGGTAGAAAATCAAAT
16 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021317.2 92.3% 92.3% 1_153del;312_338del
17 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021318.1 92.3% 92.3% 1_153del;312_338del
18 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021319.1 92.3% 92.3% 1_153del;312_338del
19 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021330.1 92.3% 92.3% 1_153del;312_338del
20 human 4253 MIA2 MIA SH3 domain ER export fa... XM_011536778.2 90.9% 90.6% (many diffs)
21 human 4253 MIA2 MIA SH3 domain ER export fa... NM_203354.3 90.8% 90.8% 1_204del;347_348insGGTAGAAAATCAAAT
22 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021316.1 90.4% 90.4% 1_204del;363_389del
23 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354150.2 90.1% 90.1% 1_222del;365_366insGGTAGAAAATCAAAT
24 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001247989.1 90.1% 90.1% 1_240del
25 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021315.2 89.7% 89.7% 1_222del;381_407del
26 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354151.2 89.5% 89.2% (many diffs)
27 human 4253 MIA2 MIA SH3 domain ER export fa... NM_005930.4 89.4% 89.4% 1_240del;383_384insGGTAGAAAATCAAAT
28 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354147.1 89.4% 89.4% 1_153del;218_219ins93
29 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021314.1 89.1% 89.1% 1_240del;399_425del
30 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354145.2 87.7% 86.9% (many diffs)
31 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354148.1 87.5% 86.7% (many diffs)
32 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001247988.1 87.3% 87.3% 1_153del;296_297insGGTAGAAAATCAAAT;1452_1453ins129
33 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354139.1 87.3% 87.3% 1_153del;296_297insGGTAGAAAATCAAAT;1452_1453ins129
34 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354157.2 86.2% 86.2% 1_240del;305_306ins93
35 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354146.2 85.4% 85.4% 1_204del;347_348insGGTAGAAAATCAAAT;1503_1504ins129
36 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354154.2 84.3% 83.6% (many diffs)
37 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354152.2 84.2% 84% (many diffs)
38 human 4253 MIA2 MIA SH3 domain ER export fa... NM_203355.3 84.1% 84.1% 1_240del;383_384insGGTAGAAAATCAAAT;1539_1540ins129
39 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354149.1 83.9% 83.9% 1_153del;218_219ins93;1374_1375ins129
40 human 64693 CTAGE1 cutaneous T cell lymphoma-a... NM_172241.3 83.9% 79.3% (many diffs)
41 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354153.2 80.9% 80.9% 1_240del;305_306ins93;1461_1462ins129
42 human 643854 CTAGE9 CTAGE family member 9 NM_001145659.1 79.4% 72.1% (many diffs)
43 human 100142659 CTAGE8 CTAGE family member 8 NM_001278507.1 79.3% 72.2% (many diffs)
44 human 100128553 CTAGE4 CTAGE family member 4 NM_198495.2 79.3% 72% (many diffs)
45 human 441294 CTAGE15 CTAGE family member 15 NM_001008747.2 79% 72% (many diffs)
46 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354155.2 79% 78.3% (many diffs)
47 human 340307 CTAGE6 CTAGE family member 6 NM_178561.5 78.9% 71.7% (many diffs)
48 human 112268382 LOC112268382 cTAGE family member 4-like XR_002959124.1 76.2% (many diffs)
49 human 647288 CTAGE11P CTAGE family member 11, pse... NR_027466.1 74.2% (many diffs)
50 human 119437 CTAGE7P CTAGE family member 7, pseu... NR_044994.1 71.2% (many diffs)
51 human 220429 CTAGE10P CTAGE family member 10, pse... NR_003268.3 67.8% (many diffs)
52 human 4253 MIA2 MIA SH3 domain ER export fa... NR_148723.2 60.8% (many diffs)
53 human 4253 MIA2 MIA SH3 domain ER export fa... NR_148722.2 59% (many diffs)
54 human 4253 MIA2 MIA SH3 domain ER export fa... NR_148721.1 57.3% (many diffs)
55 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449594.1 51.4% 51.4% 1_2064del
56 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449593.1 51.2% 51% (many diffs)
57 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449592.1 51.1% 51.1% 1_2064del;2223_2249del
58 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001329214.3 51% 51% 1_2064del;2207_2208insGGTAGAAAATCAAAT
59 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449595.1 48.1% 48.1% 1_2064del;2223_2249del;3405_3406ins129
60 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449596.1 48% 48% 1_2064del;2207_2208insGGTAGAAAATCAAAT;3363_3364ins129
61 human 4253 MIA2 MIA SH3 domain ER export fa... XR_002957555.1 35.2% (many diffs)
62 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449597.1 35% 34.2% (many diffs)
63 human 4253 MIA2 MIA SH3 domain ER export fa... XR_002957554.1 19.6% (many diffs)
64 mouse 338320 Mia2 melanoma inhibitory activity 2 NM_146034.3 76.2% 71.1% (many diffs)
65 mouse 338320 Mia2 melanoma inhibitory activity 2 NM_001329047.1 43.6% 40.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2256
ORF length:
2187
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GCTTTCTGGA CTAATTGAAG AAAAAAGTAA ACTACTTGAA AAATTTAGCC
121TTGTTCAAAA AGAGTATGAA GGCTATGAAG TAGAGTCATC TTTAAAGGAT GCCAGCTTTG
181AGAAGGAGGC AACAGAAGCA CAAAGTTTGG AGGTAGAAAA TCAAATGGCA ACCTGTGAAA
241AGCTGAACAG GTCCAATTCT GAACTTGAGG ATGAAATACT CTGTCTAGAA AAAGAGTTAA
301AAGAAGAGAA ATCCAAACAT TCTGAACAAG ATGAATTGAT GGCGGATATT TCAAAAAGGA
361TACAGTCTCT AGAAGATGAG TCAAAATCCC TCAAATCACA AGTAGCTGAA GCCAAAATGA
421CCTTCAAGAT ATTTCAAATG AATGAAGAAC GACTGAAGAT AGCAATAAAA GATGCTTTGA
481ATGAAAATTC TCAACTTCAG GAAAGCCAGA AACAGCTTTT GCAAGAAGCT GAAGTATGGA
541AAGAACAAGT GAGTGAACTT AATAAACAGA AAGTAACATT TGAAGACTCC AAAGTACATG
601CAGAACAAGT TCTAAATGAT AAAGAAAGTC ACATCAAGAC TCTGACTGAA CGCTTGTTAA
661AGATGAAAGA TTGGGCTGCT ATGCTTGGAG AAGACATAAC GGATGATGAT AACTTGGAAT
721TAGAAATGAA CAGTGAATCG GAAAATGGTG CTTACTTAGA TAATCCTCCA AAAGGAGCTT
781TGAAGAAACT GATTCATGCT GCTAAGTTAA ATGCTTCTTT AAAAACCTTA GAAGGAGAAA
841GAAACCAAAT TTATATTCAG TTGTCTGAAG TTGATAAAAC AAAGGAAGAG CTTACAGAGC
901ATATTAAAAA TCTTCAGACT GAACAAGCAT CTTTGCAGTC AGAAAACACA CATTTTGAAA
961ATGAGAATCA GAAGCTTCAA CAGAAACTTA AAGTAATGAC TGAATTATAT CAAGAAAATG
1021AAATGAAACT CCACAGGAAA TTAACAGTAG AGGAAAATTA TCGGTTAGAG AAAGAAGAGA
1081AACTTTCTAA AGTAGATGAA AAGATCAGCC ATGCCACTGA AGAGCTGGAG ACCTATAGAA
1141AGCGAGCCAA AGATCTTGAA GAAGAATTGG AGAGAACTAT TCATTCTTAT CAAGGGCAGA
1201TTATTTCCCA TGAGAAAAAA GCACATGATA ATTGGTTGGC AGCTCGGAAT GCTGAAAGAA
1261ACCTCAATGA TTTAAGGAAA GAAAATGCTC ACAACAGACA AAAATTAACT GAAACAGAGC
1321TTAAATTTGA ACTTTTAGAA AAAGATCCTT ATGCACTCGA TGTTCCAAAT ACAGCATTTG
1381GCAGAGAGCA TTCCCCATAT GGTCCCTCAC CATTGGGTTG GCCTTCATCT GAAACAAGAG
1441CTTTTCTCTC TCCTCCAACT TTGTTGGAGG GTCCACTCAG ACTCTCACCT TTGCTTCCAG
1501GGGGAGGAGG AAGAGGCTCA CGAGGCCCAG GGAATCCTCT GGACCATCAG ATTACCAATG
1561AAAGAGGAGA ATCAAGCTGT GATAGGTTAA CCGATCCTCA TAGGGCTCCC TCTGACACTG
1621GGTCTCTGTC ACCTCCATGG GACCAGGACC GTAGGATGAT GTTTCCTCCG CCAGGACAAT
1681CATATCCTGA TTCAGCCCTT CCTCCACAAA GGCAAGACAG ATTTTGTTCT AATTCTGGTA
1741GACTGTCTGG ACCAGCAGAA CTCAGAAGTT TTAATATGCC TTCTTTGGAT AAAATGGATG
1801GGTCAATGCC TTCAGAAATG GAATCCAGTA GAAATGATAC CAAAGATGAT CTTGGTAATT
1861TAAATGTGCC TGATTCATCT CTCCCTGCTG AAAATGAAGC CACTGGCCCT GGCTTTGTTC
1921CTCCACCTCT TGCTCCAATC AGAGGTCCAT TGTTTCCAGT GGATGCAAGA GGCCCATTCT
1981TGAGAAGAGG ACCTCCTTTC CCCCCACCTC CTCCAGGAGC CATGTTTGGA GCTTCTCGAG
2041ATTATTTTCC ACCAGGGGAT TTCCCAGGTC CACCACCTGC TCCATTTGCA ATGAGAAATG
2101TCTATCCACC GAGGGGTTTT CCTCCTTACC TTCCCCCAAG ACCTGGATTT TTCCCCCCAC
2161CCCCACATTC TGAAGGTAGA AGTGAGTTCC CCTCAGGTTT GATTCCACCT TCAAATGAGC
2221CTGCTACTGA ACATCCAGAA CCACAGCAAG AAACCTTGCC AACTTTCTTG TACAAAGTtg
2281gcattataag aaagcattgc ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)