Construct: ORF ccsbBroadEn_13553

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF003644.1_s300c1, BRDN0000386276
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: FRYL (285527)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	285527	FRYL	FRY like transcription coac...	XM_005248093.5	19.7%	19.7%	1_4905del;5556_5573del
2	human	285527	FRYL	FRY like transcription coac...	XM_011513684.3	19.7%	19.7%	1_4905del;5556_5573del
3	human	285527	FRYL	FRY like transcription coac...	NM_015030.2	13.3%	13.3%	1_7812del;8463_8480del
4	human	285527	FRYL	FRY like transcription coac...	XM_017008046.2	13.3%	13.3%	1_7812del;8463_8480del
5	human	285527	FRYL	FRY like transcription coac...	XM_024453993.1	13.3%	13.3%	1_7812del;8463_8480del
6	human	285527	FRYL	FRY like transcription coac...	XM_024453994.1	13.3%	13.3%	1_7812del;8463_8480del
7	human	285527	FRYL	FRY like transcription coac...	XM_005248090.5	13.3%	13.3%	1_7857del;8508_8525del
8	human	285527	FRYL	FRY like transcription coac...	XM_011513683.3	13.3%	13.3%	1_7857del;8508_8525del
9	human	285527	FRYL	FRY like transcription coac...	XM_024453990.1	13.3%	13.3%	1_7857del;8508_8525del
10	human	285527	FRYL	FRY like transcription coac...	XM_024453991.1	13.3%	13.3%	1_7857del;8508_8525del
11	human	285527	FRYL	FRY like transcription coac...	XM_024453992.1	13.3%	13.3%	1_7857del;8508_8525del
12	human	285527	FRYL	FRY like transcription coac...	XM_011513682.3	13%	13%	1_8088del
13	human	285527	FRYL	FRY like transcription coac...	XM_024453989.1	12.9%	12.9%	1_8088del;8739_8756del
14	human	285527	FRYL	FRY like transcription coac...	XM_011513681.3	12.9%	12.9%	1_8133del
15	human	285527	FRYL	FRY like transcription coac...	XM_011513680.3	12.9%	12.9%	1_8130del;8781_8798del
16	human	285527	FRYL	FRY like transcription coac...	XM_024453988.1	12.9%	12.9%	1_8133del;8784_8801del
17	mouse	72313	Fryl	FRY like transcription coac...	NM_028194.2	11.2%	12.2%	(many diffs)
18	mouse	72313	Fryl	FRY like transcription coac...	XM_006504150.3	11.2%	12.2%	(many diffs)
19	mouse	72313	Fryl	FRY like transcription coac...	XM_006504148.3	11.1%	12.1%	(many diffs)
20	mouse	72313	Fryl	FRY like transcription coac...	XM_006504149.3	11.1%	12.1%	(many diffs)
21	mouse	72313	Fryl	FRY like transcription coac...	XM_011240773.2	11.1%	12.1%	(many diffs)
22	mouse	72313	Fryl	FRY like transcription coac...	XM_006504146.3	10.9%	11.9%	(many diffs)
23	mouse	72313	Fryl	FRY like transcription coac...	XM_017321111.1	10.9%	11.8%	(many diffs)
24	mouse	72313	Fryl	FRY like transcription coac...	XM_006504152.3	10.9%	11.8%	(many diffs)
25	mouse	72313	Fryl	FRY like transcription coac...	XM_006504145.3	10.9%	11.8%	(many diffs)
26	mouse	72313	Fryl	FRY like transcription coac...	XM_006504144.3	10.8%	11.8%	(many diffs)
27	mouse	72313	Fryl	FRY like transcription coac...	XM_006504143.3	10.8%	11.8%	(many diffs)
28	mouse	72313	Fryl	FRY like transcription coac...	XM_006504141.3	10.8%	11.7%	(many diffs)
29	mouse	72313	Fryl	FRY like transcription coac...	XM_017321110.1	10.8%	11.7%	(many diffs)
30	mouse	72313	Fryl	FRY like transcription coac...	XM_006504140.3	10.8%	11.7%	(many diffs)
31	mouse	72313	Fryl	FRY like transcription coac...	XM_006504142.3	10.8%	11.7%	(many diffs)
32	mouse	72313	Fryl	FRY like transcription coac...	XM_006504138.3	10.8%	11.7%	(many diffs)
33	mouse	72313	Fryl	FRY like transcription coac...	XM_006504139.3	10.8%	11.7%	(many diffs)
34	mouse	72313	Fryl	FRY like transcription coac...	XM_006504137.3	10.8%	11.7%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1275

ORF length:

1209

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61	TTGGCATGCC AGAGCCTCTA GCTCCTGAAA GTTACCCCGA GTCAGTCTGT GAAGAGGATG
121	TTACCTTAGC TCTGAAAGAG CTAGATGAAA GATGTGAAGA AGAAGAAGCG GATTTCTCCG
181	GACTGTCTAG TCAAGATGAA GAAGAGCAAG ATGGTTTTCC AGAAGTACAG ACGTCGCCTC
241	TGCCGTCACC ATTTCTTTCT GCCATCATAG CCGCCTTTCA GCCCGTGGCA TATGATGATG
301	AAGAGGAAGC CTGGCGCTGC CACGTCAATC AGATGCTGTC TGACACCGAC GGGTCCTCTG
361	CAGTGTTTAC TTTTCATGTG TTTTCTAGGC TGTTTCAGAC AATTCAAAGA AAGTTTGGAG
421	AAATAACTAA TGAGGCAGTC AGCTTTCTTG GTGATAGTCT GCAACGCATT GGTACCAAAT
481	TTAAAAGTTC CTTGGAAGTG ATGATGCTGT GTTCAGAATG CCCAACAGTC TTTGTGGATG
541	CTGAAACACT GATGTCATGT GGTTTGCTGG AAACACTCAA GTTTGGTGTT TTGGAGTTGC
601	AAGAACACCT GGATACATAC AATGTGAAAA GAGAAGCCGC TGAGCAGTGG CTAGATGATT
661	GTAAGAGGAC ATTTGGTGCC AAAGAAGACA TGTATAGGAT AAACACAGAT GCACAAGAAT
721	TGGAGCTCTG CCGAAGATTA TACAAATTGC ATTTTCAATT GCTGCTTCTG TTCCAGGCCT
781	ACTGTAAACT TATCAACCAA GTAAATACGA TAAAAAATGA AGCAGAGGTC ATCAACATGT
841	CAGAGGAACT TGCCCAACTG GAAAGTATCC TCAAAGAAGC TGAGTCCGCT TCCGAAAACG
901	AAGAAATTGA CATTTCCAAA GCTGCACAAA CTACTATAGA AACTGCCATT CATTCTTTAA
961	TTGAAACTTT GAAAAATAAA GAATTTATAT CAGCTGTAGC ACAAGTCAAA GCTTTCAGAT
1021	CTCTCTGGCC CAGTGATATC TTTGGCAGTT GTGAAGATGA CCCTGTACAG ACACTGTTAC
1081	ATATATATTT CCATCATCAG ACGCTGGGCC AGACAGGAAG CTTTGCAGTT ATAGGCTCTA
1141	ACCTGGACAT GTCAGAAGCC AACTACAAAC TGATGGAACT TAATCTGGAA ATAAGAGAGT
1201	CTCTACGCAT GGTGCAATCA TACCAACTTC TAGCACAGGC CAAACCAATG GGAAATATGG
1261	TGAGCACTGG ATTCTGCCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct
1321	tatcaatttg ttgcaacgaa c

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