Construct: ORF ccsbBroadEn_09791

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010145.1_s300c1, BRDN0000392559
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EEF2KMT (196483)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 196483 EEF2KMT eukaryotic elongation facto... NM_201400.4 99.7% 99.6% 783G>C;814C>G
2 human 85002 FAM86B1 family with sequence simila... XM_006716256.4 94.5% 91.8% (many diffs)
3 human 653333 FAM86B2 family with sequence simila... NM_001137610.2 94.4% 91.8% (many diffs)
4 human 196483 EEF2KMT eukaryotic elongation facto... XM_005255157.4 91.6% 91.5% 156_157ins81;702G>C;733C>G
5 human 196483 EEF2KMT eukaryotic elongation facto... NM_201598.3 89.5% 89.3% 239_240ins102;712C>G
6 human 85002 FAM86B1 family with sequence simila... NM_001083537.2 85.1% 82.4% (many diffs)
7 human 196483 EEF2KMT eukaryotic elongation facto... NM_001289029.1 81.4% 81.2% 158_159ins183;631C>G
8 human 85002 FAM86B1 family with sequence simila... XM_006716257.4 78.1% 71.1% (many diffs)
9 human 85002 FAM86B1 family with sequence simila... XM_006716260.4 77.3% 74.8% (many diffs)
10 human 85002 FAM86B1 family with sequence simila... XM_017013916.2 76.6% 69.5% (many diffs)
11 human 85002 FAM86B1 family with sequence simila... XM_017013917.2 69.5% 62.6% (many diffs)
12 human 196483 EEF2KMT eukaryotic elongation facto... XM_005255158.4 69.4% 69.3% 0_1ins300;483G>C;514C>G
13 human 196483 EEF2KMT eukaryotic elongation facto... XM_011522404.2 69.4% 69.3% 0_1ins300;483G>C;514C>G
14 human 85002 FAM86B1 family with sequence simila... XM_017013918.2 66.9% 60.1% (many diffs)
15 human 286042 FAM86B3P family with sequence simila... NR_024361.1 61.6% (many diffs)
16 human 85002 FAM86B1 family with sequence simila... XM_011543843.3 59.2% 52.7% (many diffs)
17 human 85002 FAM86B1 family with sequence simila... XM_011543842.3 54.3% 40.5% (many diffs)
18 human 85002 FAM86B1 family with sequence simila... XM_024447313.1 54.3% 40.5% (many diffs)
19 human 85002 FAM86B1 family with sequence simila... XM_024447314.1 54.3% 40.5% (many diffs)
20 human 286042 FAM86B3P family with sequence simila... NR_024362.1 54% (many diffs)
21 human 85002 FAM86B1 family with sequence simila... XM_024447316.1 47.7% 30.4% (many diffs)
22 human 55199 FAM86C1 family with sequence simila... NM_018172.3 46.4% 27.7% (many diffs)
23 human 85002 FAM86B1 family with sequence simila... XM_024447315.1 45.3% 40.4% (many diffs)
24 human 55199 FAM86C1 family with sequence simila... NM_001099653.1 44.6% 26.8% (many diffs)
25 human 653333 FAM86B2 family with sequence simila... NR_148878.1 40.2% (many diffs)
26 human 85002 FAM86B1 family with sequence simila... XM_017013919.2 39% 25.5% (many diffs)
27 human 692099 FAM86DP family with sequence simila... NR_024241.1 37.2% (many diffs)
28 human 55199 FAM86C1 family with sequence simila... NM_152563.2 36.7% 19.1% (many diffs)
29 human 653333 FAM86B2 family with sequence simila... NR_148877.1 32.9% (many diffs)
30 human 85002 FAM86B1 family with sequence simila... XR_001745614.2 27.4% (many diffs)
31 human 85002 FAM86B1 family with sequence simila... XR_001745612.2 26.3% (many diffs)
32 human 85002 FAM86B1 family with sequence simila... XR_001745613.2 25% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1056
ORF length:
990
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GCCCGAGGAG AACGCGGGGA CCGAACTCTT GCTGCAGAGT TTCGAGCGCC
121GCTTCCTGGC GGCACGCACA CTGCGCTCCT TCCCCTGGCA GAGCTTAGAA GCAAAGTTAA
181GAGACTCATC AGATTCTGAG CTGCTGCGGG ATATTTTGCA CAAGACTGTG AAGCATCCTG
241TGTGTGTGAA GCACCCGCCG TCCGTCAAAT ATGCCCGGTG CTTTCTCTCA GAACTCATCA
301AAAAGCACGA GGCTGTCCAC ACAGAGCCTT TGGACGAGCT GTATGAAGCG CTGGCGGAGA
361CCCTGATGGC CAAGGAGTCC ACCCAGGGCC ACCGGAGCTA TTTGCTGCCC TCGGGAGGCT
421CGGTCACACT CTCCGAGAGC ACGGCCATCA TCTCCTACGG TACCACAGGC CTGGTCACAT
481GGGACGCCGC CCTCTACCTT GCAGAATGGG CCATCGAGAA CCCGGCAGTC TTCACTAACA
541GGACTGTCCT AGAGCTTGGC AGTGGTGCTG GCCTCACAGG CCTGGCCATC TGCAAGATGT
601GCCGCCCCCG GGCATACATC TTCAGCGACT GTCACAGCCG GGTCCTTGAG CAGCTCCGAG
661GGAATGTCCT TCTCAATGGC CTCTCATTAG AGGCAGACAT CACTGCCAAG TTAGACAGCC
721CCAGGGTGAC AGTGGCCCAG CTGGACTGGG ACGTCGCGAC GGTCCATCAG CTCTCTGCCT
781TCCAGCCAGA TGTTGTCATT GCAGCAGATG TGCTGTATTG CCCAGAAGCC ATCATGTCGC
841TGGTCGGCGT CCTGCGGAGG CTGGCTGCCT GCCGGGAGGA CCAGCGGGCT CCTGAGGTCT
901ACGTGGCCTT TACCGTCCGC AACCCAGAGA CGTGCCAGCT GTTCACCACC GAGCTAGGCC
961GGGCCGGGAT CAGATGGGAA GTGGAACCTC GTCATGAGCA GAAACTGTTT CCCTACGAAG
1021AGCACTTGGA GATGGCAATG CTGAATCTCA CCCTGTACCC AACTTTCTTG TACAAAGTtg
1081gcattataag aaagcattgc ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)