Construct: ORF TRCN0000466174
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010145.1_s317c1
- Derived from:
- ccsbBroadEn_09791
- DNA Barcode:
- GGAGAGATGACCTTCCATCATCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EEF2KMT (196483)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466174
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | NM_201400.4 | 99.7% | 99.6% | 783G>C;814C>G |
| 2 | human | 85002 | FAM86B1 | family with sequence simila... | XM_006716256.4 | 94.5% | 91.8% | (many diffs) |
| 3 | human | 653333 | FAM86B2 | family with sequence simila... | NM_001137610.2 | 94.4% | 91.8% | (many diffs) |
| 4 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | XM_005255157.4 | 91.6% | 91.5% | 156_157ins81;702G>C;733C>G |
| 5 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | NM_201598.3 | 89.5% | 89.3% | 239_240ins102;712C>G |
| 6 | human | 85002 | FAM86B1 | family with sequence simila... | NM_001083537.2 | 85.1% | 82.4% | (many diffs) |
| 7 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | NM_001289029.1 | 81.4% | 81.2% | 158_159ins183;631C>G |
| 8 | human | 85002 | FAM86B1 | family with sequence simila... | XM_006716257.4 | 78.1% | 71.1% | (many diffs) |
| 9 | human | 85002 | FAM86B1 | family with sequence simila... | XM_006716260.4 | 77.3% | 74.8% | (many diffs) |
| 10 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013916.2 | 76.6% | 69.5% | (many diffs) |
| 11 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013917.2 | 69.5% | 62.6% | (many diffs) |
| 12 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | XM_005255158.4 | 69.4% | 69.3% | 0_1ins300;483G>C;514C>G |
| 13 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | XM_011522404.2 | 69.4% | 69.3% | 0_1ins300;483G>C;514C>G |
| 14 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013918.2 | 66.9% | 60.1% | (many diffs) |
| 15 | human | 286042 | FAM86B3P | family with sequence simila... | NR_024361.1 | 61.6% | (many diffs) | |
| 16 | human | 85002 | FAM86B1 | family with sequence simila... | XM_011543843.3 | 59.2% | 52.7% | (many diffs) |
| 17 | human | 85002 | FAM86B1 | family with sequence simila... | XM_011543842.3 | 54.3% | 40.5% | (many diffs) |
| 18 | human | 85002 | FAM86B1 | family with sequence simila... | XM_024447313.1 | 54.3% | 40.5% | (many diffs) |
| 19 | human | 85002 | FAM86B1 | family with sequence simila... | XM_024447314.1 | 54.3% | 40.5% | (many diffs) |
| 20 | human | 286042 | FAM86B3P | family with sequence simila... | NR_024362.1 | 54% | (many diffs) | |
| 21 | human | 85002 | FAM86B1 | family with sequence simila... | XM_024447316.1 | 47.7% | 30.4% | (many diffs) |
| 22 | human | 55199 | FAM86C1 | family with sequence simila... | NM_018172.3 | 46.4% | 27.7% | (many diffs) |
| 23 | human | 85002 | FAM86B1 | family with sequence simila... | XM_024447315.1 | 45.3% | 40.4% | (many diffs) |
| 24 | human | 55199 | FAM86C1 | family with sequence simila... | NM_001099653.1 | 44.6% | 26.8% | (many diffs) |
| 25 | human | 653333 | FAM86B2 | family with sequence simila... | NR_148878.1 | 40.2% | (many diffs) | |
| 26 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013919.2 | 39% | 25.5% | (many diffs) |
| 27 | human | 692099 | FAM86DP | family with sequence simila... | NR_024241.1 | 37.2% | (many diffs) | |
| 28 | human | 55199 | FAM86C1 | family with sequence simila... | NM_152563.2 | 36.7% | 19.1% | (many diffs) |
| 29 | human | 653333 | FAM86B2 | family with sequence simila... | NR_148877.1 | 32.9% | (many diffs) | |
| 30 | human | 85002 | FAM86B1 | family with sequence simila... | XR_001745614.2 | 27.4% | (many diffs) | |
| 31 | human | 85002 | FAM86B1 | family with sequence simila... | XR_001745612.2 | 26.3% | (many diffs) | |
| 32 | human | 85002 | FAM86B1 | family with sequence simila... | XR_001745613.2 | 25% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1056
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcccgaggag aacgcgggga ccgaactctt gctgcagagt ttcgagcgcc 121 gcttcctggc ggcacgcaca ctgcgctcct tcccctggca gagcttagaa gcaaagttaa 181 gagactcatc agattctgag ctgctgcggg atattttgca caagactgtg aagcatcctg 241 tgtgtgtgaa gcacccgccg tccgtcaaat atgcccggtg ctttctctca gaactcatca 301 aaaagcacga ggctgtccac acagagcctt tggacgagct gtatgaagcg ctggcggaga 361 ccctgatggc caaggagtcc acccagggcc accggagcta tttgctgccc tcgggaggct 421 cggtcacact ctccgagagc acggccatca tctcctacgg taccacaggc ctggtcacat 481 gggacgccgc cctctacctt gcagaatggg ccatcgagaa cccggcagtc ttcactaaca 541 ggactgtcct agagcttggc agtggtgctg gcctcacagg cctggccatc tgcaagatgt 601 gccgcccccg ggcatacatc ttcagcgact gtcacagccg ggtccttgag cagctccgag 661 ggaatgtcct tctcaatggc ctctcattag aggcagacat cactgccaag ttagacagcc 721 ccagggtgac agtggcccag ctggactggg acgtcgcgac ggtccatcag ctctctgcct 781 tccagccaga tgttgtcatt gcagcagatg tgctgtattg cccagaagcc atcatgtcgc 841 tggtcggcgt CCTGCGGAGG CTGGCTGCCT GCCGGGAGGA CCAGCGGGCT CCTGAGGTCT 901 ACGTGGCCTT TACCGTCCGC AACCCAGAGA CGTGCCAGCT GTTCACCACC GAGCTAGGCC 961 GGGCCGGGAT CAGATGGGAA GTGGAACCTC GTCATGAGCA GAAACTGTTT CCCTACGAAG 1021 AGCACTTGGA GATGGCAATG CTGAATCTCA CCCTGTACCC AACTTTCTTG TACAAAGTGG 1081 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1141 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1201 AGGAGAGATG ACCTTCCATC ATCTGACGCG TTAAGTCgac aatcaacctc tggattacaa 1261 aatttgtgaa agatt