Construct: ORF TRCN0000491961
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021431.2_s317c1
- DNA Barcode:
- CCGTTCATGATGGCGTAAGTACGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FAM86B1 (85002)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491961
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | NM_201400.4 | 85.2% | 84.9% | (many diffs) |
| 2 | human | 85002 | FAM86B1 | family with sequence simila... | XM_006716256.4 | 80.7% | 78.2% | (many diffs) |
| 3 | human | 653333 | FAM86B2 | family with sequence simila... | NM_001137610.2 | 80.6% | 78.2% | (many diffs) |
| 4 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | XM_005255157.4 | 78.2% | 77.9% | (many diffs) |
| 5 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | NM_201598.3 | 76.5% | 76.1% | (many diffs) |
| 6 | human | 85002 | FAM86B1 | family with sequence simila... | NM_001083537.2 | 72.7% | 70.2% | (many diffs) |
| 7 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | NM_001289029.1 | 69.5% | 69.1% | (many diffs) |
| 8 | human | 85002 | FAM86B1 | family with sequence simila... | XM_006716257.4 | 67.5% | 61.4% | (many diffs) |
| 9 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013916.2 | 66.2% | 60% | (many diffs) |
| 10 | human | 85002 | FAM86B1 | family with sequence simila... | XM_006716260.4 | 66% | 63.7% | (many diffs) |
| 11 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013917.2 | 60% | 54% | (many diffs) |
| 12 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | XM_005255158.4 | 59.3% | 59% | (many diffs) |
| 13 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | XM_011522404.2 | 59.3% | 59% | (many diffs) |
| 14 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013918.2 | 57.7% | 51.9% | (many diffs) |
| 15 | human | 286042 | FAM86B3P | family with sequence simila... | NR_024362.1 | 57.1% | (many diffs) | |
| 16 | human | 286042 | FAM86B3P | family with sequence simila... | NR_024361.1 | 55.1% | (many diffs) | |
| 17 | human | 85002 | FAM86B1 | family with sequence simila... | XM_011543843.3 | 51.1% | 45.4% | (many diffs) |
| 18 | human | 85002 | FAM86B1 | family with sequence simila... | XM_011543842.3 | 47.3% | 39.7% | (many diffs) |
| 19 | human | 85002 | FAM86B1 | family with sequence simila... | XM_024447313.1 | 47.3% | 39.7% | (many diffs) |
| 20 | human | 85002 | FAM86B1 | family with sequence simila... | XM_024447314.1 | 47.3% | 39.7% | (many diffs) |
| 21 | human | 653333 | FAM86B2 | family with sequence simila... | NR_148878.1 | 44.2% | (many diffs) | |
| 22 | human | 85002 | FAM86B1 | family with sequence simila... | XM_024447316.1 | 41.3% | 30.6% | (many diffs) |
| 23 | human | 55199 | FAM86C1 | family with sequence simila... | NM_018172.3 | 39.7% | 23.9% | (many diffs) |
| 24 | human | 692099 | FAM86DP | family with sequence simila... | NR_024241.1 | 39.7% | (many diffs) | |
| 25 | human | 85002 | FAM86B1 | family with sequence simila... | XM_024447315.1 | 39.5% | 34.9% | (many diffs) |
| 26 | human | 55199 | FAM86C1 | family with sequence simila... | NM_001099653.1 | 38.1% | 23% | (many diffs) |
| 27 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013919.2 | 33.4% | 22% | (many diffs) |
| 28 | human | 55199 | FAM86C1 | family with sequence simila... | NM_152563.2 | 31.4% | 16.4% | (many diffs) |
| 29 | human | 653333 | FAM86B2 | family with sequence simila... | NR_148877.1 | 29.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 1242
- ORF length:
- 1158
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcgg 61 ccgccccctt caccggatcc gtcatggcgc ccgaggagaa cgcggggacc gaactcttgc 121 tgcagagttt cgagcgccgc ttcctggcgg cacgcacact gcgctccttc ccctggcagg 181 tgggcggcgg ggcgagcgga gaggcccgcg gggctcgcgg gagtccaggg gcagacggga 241 cgggtctccg tgctgaagcc cctggcgctc ccgccactaa tcgttctggt cactgggaca 301 cagctgccct cgcccattct aaaaagtcag cgccgtcagg accgcagagc ttagaagcaa 361 agttaagaga ctcatcagat tctgagctgc tgcgggatat tttgcacaag actgtgaagc 421 atcctgtgtg tgtgaagcac ccgccgtccg tcaaatatgc ccggtgcttt ctctcagaac 481 tcatcaaaaa gcacgaggct gtccacacag agcctttgga cgagctgtat gaagcgctgg 541 cggagaccct gatggccaag gagtccaccc agggccaccg gagctatttg ctgccctcgg 601 gaggctcggt cacactctcc gagagcacgg ccatcatctc ctacggtacc acaggcctgg 661 tcacatggga cgccgccctc taccttgcag aatgggccat cgagaacccg gcagtcttca 721 ctaacaggac tgtcctagag cttggcagtg gtgttggcct cacaggcctg gccatctgca 781 agatgtgccg cccccgggca tacatcttca gcgactgtca cagccgggtc cttgagcagc 841 tccgagggaa tgtccttctc aatggCCTCT CATTAGAGGC AGACATCACT GCCAAGTTAG 901 ACAGCCCCAG GGTGACAGTG GCCCAGCTGG ACTGGGACGT CGCGACGGTC CATCAGCTCT 961 CTGCCTTCCA GCCAGATGTT GTCATTGCAG CAGATGTGCT GTATTGCCCA GAAGCCATCA 1021 TGTCGCTGGT CGGCGTCCTG CGGAGGCTGG CTGCCTGCCG GGAGGACCAG CGGGCTCCTG 1081 AGGTCTACGT GGCCTTTACC GTCCGCAACC CAGAGACGTG CCAGCTGTTC ACCACCGAGC 1141 TAGGCCGGGC CGGGATCAGA TGGGAAGTGG AACCTCGTCA TGAGCAGAAA CTGTTTCCCT 1201 ACGAAGAGCA CTTGGAGATG GCAATGCTGA ATCTCACCCT GTGAAAGGGT GGGCGCGCCG 1261 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1321 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1381 TTTATATATC TTGTGGAAAG GACGACCGTT CATGATGGCG TAAGTACGCA CGCGTTAAGT 1441 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt