Construct: ORF TRCN0000488297

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021433.1_s317c1
DNA Barcode:
ACTAGACGAAAAATGAGGCATGGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
FAM86B1 (85002)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488297
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 196483 EEF2KMT eukaryotic elongation facto... NM_201400.4 99.7% 99.6% 783G>C;814C>G
2 human 85002 FAM86B1 family with sequence simila... XM_006716256.4 94.5% 91.8% (many diffs)
3 human 653333 FAM86B2 family with sequence simila... NM_001137610.2 94.4% 91.8% (many diffs)
4 human 196483 EEF2KMT eukaryotic elongation facto... XM_005255157.4 91.6% 91.5% 156_157ins81;702G>C;733C>G
5 human 196483 EEF2KMT eukaryotic elongation facto... NM_201598.3 89.5% 89.3% 239_240ins102;712C>G
6 human 85002 FAM86B1 family with sequence simila... NM_001083537.2 85.1% 82.4% (many diffs)
7 human 196483 EEF2KMT eukaryotic elongation facto... NM_001289029.1 81.4% 81.2% 158_159ins183;631C>G
8 human 85002 FAM86B1 family with sequence simila... XM_006716257.4 78.1% 71.1% (many diffs)
9 human 85002 FAM86B1 family with sequence simila... XM_006716260.4 77.3% 74.8% (many diffs)
10 human 85002 FAM86B1 family with sequence simila... XM_017013916.2 76.6% 69.5% (many diffs)
11 human 85002 FAM86B1 family with sequence simila... XM_017013917.2 69.5% 62.6% (many diffs)
12 human 196483 EEF2KMT eukaryotic elongation facto... XM_005255158.4 69.4% 69.3% 0_1ins300;483G>C;514C>G
13 human 196483 EEF2KMT eukaryotic elongation facto... XM_011522404.2 69.4% 69.3% 0_1ins300;483G>C;514C>G
14 human 85002 FAM86B1 family with sequence simila... XM_017013918.2 66.9% 60.1% (many diffs)
15 human 286042 FAM86B3P family with sequence simila... NR_024361.1 61.6% (many diffs)
16 human 85002 FAM86B1 family with sequence simila... XM_011543843.3 59.2% 52.7% (many diffs)
17 human 85002 FAM86B1 family with sequence simila... XM_011543842.3 54.3% 40.5% (many diffs)
18 human 85002 FAM86B1 family with sequence simila... XM_024447313.1 54.3% 40.5% (many diffs)
19 human 85002 FAM86B1 family with sequence simila... XM_024447314.1 54.3% 40.5% (many diffs)
20 human 286042 FAM86B3P family with sequence simila... NR_024362.1 54% (many diffs)
21 human 85002 FAM86B1 family with sequence simila... XM_024447316.1 47.7% 30.4% (many diffs)
22 human 55199 FAM86C1 family with sequence simila... NM_018172.3 46.4% 27.7% (many diffs)
23 human 85002 FAM86B1 family with sequence simila... XM_024447315.1 45.3% 40.4% (many diffs)
24 human 55199 FAM86C1 family with sequence simila... NM_001099653.1 44.6% 26.8% (many diffs)
25 human 653333 FAM86B2 family with sequence simila... NR_148878.1 40.2% (many diffs)
26 human 85002 FAM86B1 family with sequence simila... XM_017013919.2 39% 25.5% (many diffs)
27 human 692099 FAM86DP family with sequence simila... NR_024241.1 37.2% (many diffs)
28 human 55199 FAM86C1 family with sequence simila... NM_152563.2 36.7% 19.1% (many diffs)
29 human 653333 FAM86B2 family with sequence simila... NR_148877.1 32.9% (many diffs)
30 human 85002 FAM86B1 family with sequence simila... XR_001745614.2 27.4% (many diffs)
31 human 85002 FAM86B1 family with sequence simila... XR_001745612.2 26.3% (many diffs)
32 human 85002 FAM86B1 family with sequence simila... XR_001745613.2 25% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
84
ORF end:
1074
ORF length:
990
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcgg
61ccgccccctt caccggatcc gtcatggcgc ccgaggagaa cgcggggacc gaactcttgc
121tgcagagttt cgagcgccgc ttcctggcgg cacgcacact gcgctccttc ccctggcaga
181gcttagaagc aaagttaaga gactcatcag attctgagct gctgcgggat attttgcaca
241agactgtgaa gcatcctgtg tgtgtgaagc acccgccgtc cgtcaaatat gcccggtgct
301ttctctcaga actcatcaaa aagcacgagg ctgtccacac agagcctttg gacgagctgt
361atgaagcgct ggcggagacc ctgatggcca aggagtccac ccagggccac cggagctatt
421tgctgccctc gggaggctcg gtcacactct ccgagagcac ggccatcatc tcctacggta
481ccacaggcct ggtcacatgg gacgccgccc tctaccttgc agaatgggcc atcgagaacc
541cggcagtctt cactaacagg actgtcctag agcttggcag tggtgctggc ctcacaggcc
601tggccatctg caagatgtgc cgcccccggg catacatctt cagcgactgt cacagccggg
661tccttgagca gctccgaggg aatgtccttc tcaatggcct ctcattagag gcagacatca
721ctgccaagtt agacagcccc agggtgacag tggcccagct ggactgggac gtcgcgacgg
781tccaTCAGCT CTCTGCCTTC CAGCCAGATG TTGTCATTGC AGCAGATGTG CTGTATTGCC
841CAGAAGCCAT CATGTCGCTG GTCGGCGTCC TGCGGAGGCT GGCTGCCTGC CGGGAGGACC
901AGCGGGCTCC TGAGGTCTAC GTGGCCTTTA CCGTCCGCAA CCCAGAGACG TGCCAGCTGT
961TCACCACCGA GCTAGGCCGG GCCGGGATCA GATGGGAAGT GGAACCTCGT CATGAGCAGA
1021AACTGTTTCC CTACGAAGAG CACTTGGAGA TGGCAATGCT GAATCTCACC CTGTGAAAGG
1081GTGGGCGCGC CGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1141ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1201CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACT AGACGAAAAA TGAGGCATGG
1261AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)