Transcript: Human NM_001130679.2

Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
EIF4E (1977)
Length:
11626
CDS:
1526..2272

Additional Resources:

NCBI RefSeq record:
NM_001130679.2
NBCI Gene record:
EIF4E (1977)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001130679.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000062576 CGGCTGATCTCCAAGTTTGAT pLKO.1 1706 CDS 100% 5.625 7.875 N EIF4E n/a
2 TRCN0000310424 CGGCTGATCTCCAAGTTTGAT pLKO_005 1706 CDS 100% 5.625 7.875 N EIF4E n/a
3 TRCN0000062574 CCAAAGATAGTGATTGGTTAT pLKO.1 2189 CDS 100% 10.800 7.560 N EIF4E n/a
4 TRCN0000299520 CCAAAGATAGTGATTGGTTAT pLKO_005 2189 CDS 100% 10.800 7.560 N EIF4E n/a
5 TRCN0000062573 CCACTCTGTAATAGTTCAGTA pLKO.1 2537 3UTR 100% 4.950 3.465 N EIF4E n/a
6 TRCN0000299577 CCACTCTGTAATAGTTCAGTA pLKO_005 2537 3UTR 100% 4.950 3.465 N EIF4E n/a
7 TRCN0000062575 CCGACTACAGAAGAGGAGAAA pLKO.1 1568 CDS 100% 4.950 3.465 N EIF4E n/a
8 TRCN0000299576 CCGACTACAGAAGAGGAGAAA pLKO_005 1568 CDS 100% 4.950 3.465 N EIF4E n/a
9 TRCN0000062577 CTGTTGTTAATGTTAGAGCTA pLKO.1 2073 CDS 100% 2.640 1.848 N EIF4E n/a
10 TRCN0000204533 CCTCCCAAAGTGCTGGAATTA pLKO.1 1994 CDS 100% 13.200 6.600 Y LRRC74B n/a
11 TRCN0000008902 CCTCCCAAAGTGTTGGGATTA pLKO.1 9735 3UTR 100% 1.080 0.540 Y GPR83 n/a
12 TRCN0000156315 CCTCCCAAAGTGTTGGGATTA pLKO.1 9735 3UTR 100% 1.080 0.540 Y MYORG n/a
13 TRCN0000172586 GCCTGGCCAACATGATGAAAT pLKO.1 8294 3UTR 100% 13.200 6.600 Y SPIRE2 n/a
14 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 6234 3UTR 100% 13.200 6.600 Y LIAS n/a
15 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4903 3UTR 100% 5.625 2.813 Y KLHL30 n/a
16 TRCN0000161892 GCCTGTAATTCCAGCTACTTA pLKO.1 7201 3UTR 100% 5.625 2.813 Y GPN2 n/a
17 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 8750 3UTR 100% 4.950 2.475 Y ERAP2 n/a
18 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4903 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001130679.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06150 pDONR223 100% 87.2% 87% None 241T>C;379G>A;400_492del n/a
2 ccsbBroad304_06150 pLX_304 93.5% 87.2% 87% V5 241T>C;379G>A;400_492del n/a
3 TRCN0000471416 AGCTTATAGAAACCCTCGGGAACC pLX_317 54% 87.2% 87% V5 241T>C;379G>A;400_492del n/a
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