Transcript: Mouse NM_001271370.1

Mus musculus dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1b (Dyrk1b), transcript variant 4, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Dyrk1b (13549)
Length:
2477
CDS:
33..2102

Additional Resources:

NCBI RefSeq record:
NM_001271370.1
NBCI Gene record:
Dyrk1b (13549)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001271370.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000435463 ACATCAATGAGGTATACTATG pLKO_005 385 CDS 100% 10.800 15.120 N Dyrk1b n/a
2 TRCN0000416984 TACTACATAGTACACCTTAAG pLKO_005 723 CDS 100% 10.800 15.120 N Dyrk1b n/a
3 TRCN0000412749 ACAGATTGAGCTACGGCTGTT pLKO_005 668 CDS 100% 4.050 5.670 N Dyrk1b n/a
4 TRCN0000023027 CTACCGATACAGCAACCGATA pLKO.1 1658 CDS 100% 4.050 5.670 N Dyrk1b n/a
5 TRCN0000418794 GTGGACCAGATGAGCCGTATT pLKO_005 1167 CDS 100% 10.800 8.640 N Dyrk1b n/a
6 TRCN0000023025 CCAGCGGATCTACCAGTATAT pLKO.1 1013 CDS 100% 13.200 9.240 N Dyrk1b n/a
7 TRCN0000417765 GTGCTGCGCATGCTGGAATAT pLKO_005 1434 CDS 100% 13.200 9.240 N Dyrk1b n/a
8 TRCN0000433605 ATCACCTGTGCCTGGTGTTTG pLKO_005 763 CDS 100% 10.800 7.560 N Dyrk1b n/a
9 TRCN0000427288 CCAAGGCTCGAAAGTACTTTG pLKO_005 1234 CDS 100% 10.800 7.560 N Dyrk1b n/a
10 TRCN0000432195 CCTATCTCTGCCTCAACATTG pLKO_005 1797 CDS 100% 10.800 7.560 N Dyrk1b n/a
11 TRCN0000419572 ATGACCTGGCCATTGACATGT pLKO_005 1081 CDS 100% 4.950 3.465 N Dyrk1b n/a
12 TRCN0000023028 CCAGCATGATACAGAGATGAA pLKO.1 701 CDS 100% 4.950 3.465 N Dyrk1b n/a
13 TRCN0000002139 GACCTACAAGCACATCAATGA pLKO.1 374 CDS 100% 4.950 3.465 N DYRK1B n/a
14 TRCN0000023024 GCTATGAGATTGACTCTCTTA pLKO.1 541 CDS 100% 4.950 3.465 N Dyrk1b n/a
15 TRCN0000023026 CCACGGTTATGATGACGACAA pLKO.1 479 CDS 100% 4.050 2.835 N Dyrk1b n/a
16 TRCN0000199056 CCCTCCGGACTCGGATGACTG pLKO.1 1984 CDS 100% 0.000 0.000 N DYRK1B n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001271370.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14931 pDONR223 0% 84.5% 88.8% None (many diffs) n/a
2 ccsbBroad304_14931 pLX_304 0% 84.5% 88.8% V5 (many diffs) n/a
3 TRCN0000479503 CGCACGTACGGTCGGCCCGAATGT pLX_317 16.1% 84.5% 88.8% V5 (many diffs) n/a
4 TRCN0000489760 AATATGTTGATGCCGAGACCTTCC pLX_317 16.8% 78.8% 83% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV