Construct: ORF TRCN0000479503
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005577.3_s317c1
- Derived from:
- ccsbBroadEn_14931
- DNA Barcode:
- CGCACGTACGGTCGGCCCGAATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYRK1B (9149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479503
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9149 | DYRK1B | dual specificity tyrosine p... | NM_004714.3 | 100% | 100% | |
| 2 | human | 9149 | DYRK1B | dual specificity tyrosine p... | XM_005259398.4 | 100% | 100% | |
| 3 | human | 9149 | DYRK1B | dual specificity tyrosine p... | NM_006484.2 | 95.5% | 95.5% | 1129_1130ins84 |
| 4 | human | 9149 | DYRK1B | dual specificity tyrosine p... | NM_006483.3 | 93.6% | 93.6% | 1093_1094ins120 |
| 5 | mouse | 13549 | Dyrk1b | dual-specificity tyrosine-(... | NM_001037957.3 | 92.6% | 97.2% | (many diffs) |
| 6 | mouse | 13549 | Dyrk1b | dual-specificity tyrosine-(... | NM_010092.2 | 86.3% | 90.9% | (many diffs) |
| 7 | mouse | 13549 | Dyrk1b | dual-specificity tyrosine-(... | NM_001271370.1 | 84.5% | 88.8% | (many diffs) |
| 8 | mouse | 13549 | Dyrk1b | dual-specificity tyrosine-(... | XM_006539526.3 | 80.5% | 84.7% | (many diffs) |
| 9 | mouse | 13549 | Dyrk1b | dual-specificity tyrosine-(... | XM_006539527.3 | 78.8% | 83% | (many diffs) |
| 10 | mouse | 13549 | Dyrk1b | dual-specificity tyrosine-(... | XM_006539528.3 | 41.7% | 36.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1953
- ORF length:
- 1887
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgtcccaccg ggccatggtc ccttctctgg cttcccaggg ccccaggagc 121 acacgcaggt attgcctgat gtgcggctac tgcctcggag gctgcccctg gccttccggg 181 atgcaacctc agccccgctg cgtaagctct ctgtggacct catcaagacc tacaagcaca 241 tcaatgaggt atactatgcg aagaagaagc ggcgggccca gcaggcgcca ccccaggatt 301 cgagcaacaa gaaggagaag aaggtcctga accatggtta tgatgacgac aaccatgact 361 acatcgtgcg cagtggcgag cgctggctgg agcgctacga aattgactcg ctcattggca 421 aaggctcctt tggccaggtg gtgaaagcct atgatcatca gacccaggag cttgtggcca 481 tcaagatcat caagaacaaa aaggctttcc tgaaccaggc ccagattgag ctgcggctgc 541 tggagctgat gaaccagcat gacacggaga tgaagtacta tatagtacac ctgaagcggc 601 acttcatgtt ccggaaccac ctgtgcctgg tatttgagct gctgtcctac aacctgtacg 661 acctcctgcg caacacccac ttccgcggcg tctcgctgaa cctgacccgg aagctggcgc 721 agcagctctg cacggcactg ctctttctgg ccacgcctga gctcagcatc attcactgcg 781 acctcaagcc cgaaaacatc ttgctgtgca accccaagcg cagcgccatc aagattgtgg 841 acttcggcag ctcctgccag cttggccaga ggatctacca gtatatccag agccgcttct 901 accgctcacc tgaggtgctc ctgggcacac cctacgacct ggccattgac atgtggtccc 961 tgggctgcat ccttgtggag atgcacaccg gagagcccct cttcagtggc tccaatgagg 1021 tcgaccagat gaaccgcatt gtggaggtgc tgggcatccc accggccgcc atgctggacc 1081 aggcgcccaa ggctcgcaag tactttgaac ggctgcctgg gggtggctgg accctacgaa 1141 ggacgaaaga actcaggaag gattaccagg gccccgggac acggcggctg caggaggtgc 1201 tgggcgtgca gacgggcggg cccgggggcc ggcgggcggg ggagccgggc cacagccccg 1261 ccgactacct ccgcttccag gacctggtgc tgcgcatgct ggagtatgag cccgccgccc 1321 gcatcagccc cctgggggct ctgcagcacg gcttcttccg ccgcacggcc gacgaggcca 1381 ccaacacggg cccggcaggc agcagtgcct ccacctcgcc cgcgcccctc gacacctgcc 1441 cctcttccag caccgccagc tccatctcca gttctggagg ctccagtggc tcctccagtg 1501 acaaccggac ctaccgctac agcaaccgat attgtggggg ccctgggccc cctatcacag 1561 actgtgagat gaacagcccc caggtcccac cctcccagcc gctgcggccc tgggcagggg 1621 gtgatgtgcc ccacaagaca catcaagccc ctgccTCTGC CTCGTCACTG CCTGGGACCG 1681 GGGCCCAGTT ACCCCCCCAG CCCCGATACC TTGGTCGTCC CCCATCACCA ACCTCACCAC 1741 CACCCCCGGA GCTGATGGAT GTGAGCCTGG TGGGCGGCCC TGCTGACTGC TCCCCACCTC 1801 ACCCAGCGCC TGCCCCCCAG CACCCGGCTG CCTCAGCCCT CCGGACTCGG ATGACTGGAG 1861 GTCGTCCACC CCTCCCGCCT CCTGATGACC CTGCCACTCT GGGGCCTCAC CTGGGCCTCC 1921 GTGGTGTACC CCAGAGCACA GCAGCCAGCT CGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1981 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2041 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 2101 CACGTACGGT CGGCCCGAAT GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2161 ttgtgaaaga tt