Transcript: Human NM_001282484.1

Homo sapiens golgin A8 family member R (GOLGA8R), mRNA.

Source:
NCBI, updated 2019-05-01
Taxon:
Homo sapiens (human)
Gene:
GOLGA8R (101059918)
Length:
5172
CDS:
99..1994

Additional Resources:

NCBI RefSeq record:
NM_001282484.1
NBCI Gene record:
GOLGA8R (101059918)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001282484.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000151320 GCAGGAGATTTGCACATTAAA pLKO.1 878 CDS 100% 15.000 7.500 Y GOLGA8IP n/a
2 TRCN0000153447 CGCAGGAGATTTGCACATTAA pLKO.1 877 CDS 100% 13.200 6.600 Y GOLGA8IP n/a
3 TRCN0000162932 GCAGTTGGAGCAGCAAGTAAA pLKO.1 1250 CDS 100% 13.200 6.600 Y GOLGA8B n/a
4 TRCN0000269218 CAGTTGGAGCAGCAAGTAAAG pLKO_005 1251 CDS 100% 10.800 5.400 Y GOLGA6L9 n/a
5 TRCN0000153167 GCCTATGTTCTGCTGTTGTTT pLKO.1 2400 3UTR 100% 5.625 2.813 Y GOLGA8G n/a
6 TRCN0000152586 GACACAGTTGAAGGAGTCATT pLKO.1 770 CDS 100% 4.950 2.475 Y GOLGA8IP n/a
7 TRCN0000153184 GTGGGAGTTCAAACACACAAA pLKO.1 2273 3UTR 100% 4.950 2.475 Y GOLGA8F n/a
8 TRCN0000154490 GCAGCAAGTAAAGGAGCTACA pLKO.1 1259 CDS 100% 4.050 2.025 Y GOLGA8IP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001282484.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16170 pDONR223 0% 59.2% 52.3% None (many diffs) n/a
2 ccsbBroad304_16170 pLX_304 0% 59.2% 52.3% V5 (many diffs) n/a
3 ccsbBroadEn_10341 pDONR223 100% 58.8% 51.5% None (many diffs) n/a
4 ccsbBroad304_10341 pLX_304 0% 58.8% 51.5% V5 (many diffs) n/a
5 TRCN0000476380 AGTGATAACGTTCAAGGTCGCGGA pLX_317 28.7% 58.8% 51.5% V5 (many diffs) n/a
6 ccsbBroadEn_16172 pDONR223 0% 38.7% 33.6% None (many diffs) n/a
7 ccsbBroad304_16172 pLX_304 0% 38.7% 33.6% V5 (many diffs) n/a
8 TRCN0000480289 TACTAACTTATTTTTTGACAAACC pLX_317 100% 8.8% 7.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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