Construct: ORF TRCN0000476380

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014279.1_s317c1
Derived from:
ccsbBroadEn_10341
DNA Barcode:
AGTGATAACGTTCAAGGTCGCGGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GOLGA8G (283768)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476380
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 283768 GOLGA8G golgin A8 family member G NM_001368080.1 100% 100%
2 human 100132565 GOLGA8F golgin A8 family member F XM_024449812.1 100% 100%
3 human 100132565 GOLGA8F golgin A8 family member F XM_024449813.1 100% 100%
4 human 283768 GOLGA8G golgin A8 family member G NM_001368078.1 99% 99% 585_596del
5 human 100132565 GOLGA8F golgin A8 family member F XM_024449810.1 95.3% 95.3% 753_815del
6 human 100132565 GOLGA8F golgin A8 family member F XM_024449811.1 95.3% 95.3% 753_815del
7 human 283768 GOLGA8G golgin A8 family member G XM_024449906.1 95.3% 95.3% 753_815del
8 human 283768 GOLGA8G golgin A8 family member G XM_024449907.1 95.3% 95.3% 753_815del
9 human 653061 GOLGA8S golgin A8 family member S NM_001355465.1 90.4% 85.6% (many diffs)
10 human 653061 GOLGA8S golgin A8 family member S XM_024450030.1 90.4% 85.6% (many diffs)
11 human 653720 GOLGA8M golgin A8 family member M XM_011521951.3 78.5% 71.1% (many diffs)
12 human 653720 GOLGA8M golgin A8 family member M XM_011521954.3 78.5% 71.1% (many diffs)
13 human 653720 GOLGA8M golgin A8 family member M XM_024450037.1 78.5% 71.1% (many diffs)
14 human 653075 GOLGA8T golgin A8 family member T XM_024450034.1 77.8% 69.4% (many diffs)
15 human 653075 GOLGA8T golgin A8 family member T XM_024450035.1 77.8% 69.4% (many diffs)
16 human 728047 GOLGA8O golgin A8 family member O XM_024450042.1 77.6% 68.8% (many diffs)
17 human 23015 GOLGA8A golgin A8 family member A XM_017022020.2 71.6% 63.8% (many diffs)
18 human 653720 GOLGA8M golgin A8 family member M XM_024450036.1 68.6% 62.1% (many diffs)
19 human 653075 GOLGA8T golgin A8 family member T XM_024450033.1 68% 60.7% (many diffs)
20 human 283768 GOLGA8G golgin A8 family member G NM_001350919.2 66.3% 66.3% 1_654del
21 human 100132565 GOLGA8F golgin A8 family member F NM_001350920.1 66.3% 66.3% 1_654del
22 human 100132565 GOLGA8F golgin A8 family member F XM_024449809.1 64.3% 64.3% 1_651del;1404_1466del
23 human 283768 GOLGA8G golgin A8 family member G XM_024449905.1 64.3% 64.3% 1_651del;1404_1466del
24 human 100132565 GOLGA8F golgin A8 family member F XM_024449808.1 64.2% 64.2% 1_654del;1407_1469del
25 human 283768 GOLGA8G golgin A8 family member G XM_024449904.1 64.2% 64.2% 1_654del;1407_1469del
26 human 653061 GOLGA8S golgin A8 family member S XM_024450031.1 64% 55.2% (many diffs)
27 human 653720 GOLGA8M golgin A8 family member M XM_006720654.3 61.2% 55.3% (many diffs)
28 human 653073 GOLGA8J golgin A8 family member J XM_006720651.3 61.1% 54.8% (many diffs)
29 human 653075 GOLGA8T golgin A8 family member T XM_024450032.1 60.6% 54% (many diffs)
30 human 653720 GOLGA8M golgin A8 family member M NM_001282468.1 59.9% 54.2% (many diffs)
31 human 653073 GOLGA8J golgin A8 family member J NM_001282472.1 59.8% 53.7% (many diffs)
32 human 728498 GOLGA8H golgin A8 family member H NM_001282490.1 59.7% 53.4% (many diffs)
33 human 653075 GOLGA8T golgin A8 family member T NM_001355469.1 59.4% 52.9% (many diffs)
34 human 728047 GOLGA8O golgin A8 family member O NM_001277308.1 59.2% 52.5% (many diffs)
35 human 643699 GOLGA8N golgin A8 family member N NM_001282494.1 59.2% 52.5% (many diffs)
36 human 653125 GOLGA8K golgin A8 family member K NM_001282493.1 59.1% 52.9% (many diffs)
37 human 727909 GOLGA8Q golgin A8 family member Q NM_001355476.1 59.1% 52.2% (many diffs)
38 human 101059918 GOLGA8R golgin A8 family member R NM_001282484.1 58.8% 51.5% (many diffs)
39 human 728047 GOLGA8O golgin A8 family member O XM_011521988.3 55.9% 49.2% (many diffs)
40 human 653720 GOLGA8M golgin A8 family member M XM_005268284.5 55.9% 50.5% (many diffs)
41 human 23015 GOLGA8A golgin A8 family member A NM_181077.4 55.5% 49.5% (many diffs)
42 human 23015 GOLGA8A golgin A8 family member A NM_001368071.2 55.5% 49.5% (many diffs)
43 human 23015 GOLGA8A golgin A8 family member A NM_001368072.1 55.5% 49.5% (many diffs)
44 human 440270 GOLGA8B golgin A8 family member B NM_001023567.5 55.5% 49.3% (many diffs)
45 human 653720 GOLGA8M golgin A8 family member M XM_005268285.5 52.8% 47.5% (many diffs)
46 human 100132565 GOLGA8F golgin A8 family member F XR_002957623.1 42.6% 1_716del;1376_1377ins92;1571_1572ins343
47 human 283768 GOLGA8G golgin A8 family member G XR_002957634.1 42.6% 1_716del;1376_1377ins92;1571_1572ins343
48 human 653720 GOLGA8M golgin A8 family member M XM_011521952.3 39.3% 31.5% (many diffs)
49 human 101930434 LOC101930434 putative golgin subfamily A... XM_017030255.2 33.1% 28% (many diffs)
50 human 101930434 LOC101930434 putative golgin subfamily A... XM_017030256.2 33.1% 28% (many diffs)
51 human 101930434 LOC101930434 putative golgin subfamily A... XM_017030316.2 33.1% 28% (many diffs)
52 human 101930434 LOC101930434 putative golgin subfamily A... XM_017030317.2 33.1% 28% (many diffs)
53 human 653073 GOLGA8J golgin A8 family member J XM_011521950.2 33% 27.5% (many diffs)
54 human 101930434 LOC101930434 putative golgin subfamily A... XM_017022809.1 32.9% 27.5% (many diffs)
55 human 653073 GOLGA8J golgin A8 family member J XR_002957674.1 31.9% (many diffs)
56 human 653720 GOLGA8M golgin A8 family member M XM_011521953.3 30.7% 25.8% (many diffs)
57 human 100132565 GOLGA8F golgin A8 family member F NR_033351.1 28.5% 1_1015del;2306_4511del
58 human 390535 GOLGA8EP golgin A8 family member E, ... NR_033350.1 24.1% (many diffs)
59 human 729786 GOLGA8CP golgin A8 family member C, ... NR_027411.2 24% (many diffs)
60 human 100132979 GOLGA8DP golgin A8 family member D, ... NR_027407.1 23.9% (many diffs)
61 human 440270 GOLGA8B golgin A8 family member B NR_027410.1 16% (many diffs)
62 human 23015 GOLGA8A golgin A8 family member A NR_027409.2 15.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1359
ORF length:
1290
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagtggaag ttagagcagt ccatgcggga gcaggcactg ctgaaagcgc
121agctgacaca gttgaaggag tcacttaaag aagtccagct agagagggat gaatatgctg
181aacatctaaa aggagagagg gcccggtggc agcagaggat gagaaaaatg tcgcaggagg
241tttgctcgtt gaagaaggag aagaagcatg ataaatatcg ggtagagaag ctggagagga
301gcttgtccaa actcaaacac cagatggctg aacctctgcc cccggagccc ccagcagtgc
361cctctgaggt ggagctgcag cacctgagga aggaactaga gagagtggca ggagagctcc
421aggcccaggt ggagtacaat cagcgcataa gtctcctgaa tgaggggcaa aaggagaggc
481ttcgggagca ggaggagagg cttcaggagc agcaggagag gcttccagag caggaggaga
541ggcttcagca gctggccgag ccacagaaca gcttcaagga gctgaacaat gagaacaaga
601gcgtactaca gttggagcag caagtaaagg agctgcagga gaagctaggc aaggagcgtc
661tggaagctgc gagccagcag aaacagcagc taacggccca gttgagcctc atggctctcc
721ctggggaagg agatggagga ggacatctgg acagtgaggg ggaggaggca cctcggccca
781ttcctagcat cccacaggac ctggagagca gggaggccat gagcggcttt atggaccacc
841tggaggagaa ggcagacctg agtgagctgg tggagaaaga agaacttgga ttcttccagt
901actacagaga gagatgccat cagaaagttt atcaccctat aacaaagcca gggggcagtg
961ccaaagatgc agcaccggga ggaggacacc aTCAGGCTGG CCCTGGACAG GGAGGAGATG
1021AAGGTGAAGC TGCTGGAGCT GCAGGAGATG GTGTTGCAGC TGGTGGCGAC TACAAGGGAC
1081ACAGCAAATT CTTGGTGACT GCCCAGAACC CTGCTCATGA GCCCAGTCCA GGAGCCCCAG
1141CCCCCCAGGA GCTTGGGGCT GCCCACAAGC ATGGTGATCT TTGTGAGGTG AGCCTCACTG
1201ACAGCGTGGA GCCTGTGCAA GGAGAGGCCA GGGAGGGTTC TCCCCACGAC AACCCTACTG
1261CACAGCCGAT CGTGCAGGAC CACCAGGAGC ACCCAGGCTT GGGCAGCAAC TGCTGTGTGC
1321CATTCTTTTG CTGGGCTTGG CTGCCAAGAA GAAGGAGATT GCCAACTTTC TTGTACAAAG
1381TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1441GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1501ACGAAGTGAT AACGTTCAAG GTCGCGGAAC GCGTTAAGTC gacaatcaac ctctggatta
1561caaaatttgt gaaagatt

Download FASTA (ORF) (Full)