Transcript: Human NM_001367572.1

Homo sapiens zinc finger protein 138 (ZNF138), transcript variant 10, mRNA.

Source:
NCBI, updated 2018-12-08
Taxon:
Homo sapiens (human)
Gene:
ZNF138 (7697)
Length:
2642
CDS:
376..1164

Additional Resources:

NCBI RefSeq record:
NM_001367572.1
NBCI Gene record:
ZNF138 (7697)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001367572.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000429634 TAACAACTTACTGAACATAAG pLKO_005 1162 CDS 100% 10.800 7.560 N ZNF138 n/a
2 TRCN0000012919 CTGGTCCACAAACCTTTCTAA pLKO.1 819 CDS 100% 5.625 3.938 N ZNF138 n/a
3 TRCN0000012922 CACAAACCTTTCTAAACCTAA pLKO.1 825 CDS 100% 4.950 3.465 N ZNF138 n/a
4 TRCN0000414608 GCGGAATGTATATAGGCATGT pLKO_005 275 5UTR 100% 4.050 2.835 N ZNF138 n/a
5 TRCN0000421902 ACAATGTGGCAAGGTCTTTAA pLKO_005 1050 CDS 100% 13.200 7.920 N ZNF138 n/a
6 TRCN0000434325 ACACTGTGGCAAAGCCTTTAA pLKO_005 966 CDS 100% 13.200 7.920 N ZNF138 n/a
7 TRCN0000424428 ACCTTACTAGACATAAGATAA pLKO_005 998 CDS 100% 13.200 7.920 N ZNF138 n/a
8 TRCN0000413885 AGCTCACTGAACATAAGTTAA pLKO_005 1334 3UTR 100% 13.200 7.920 N ZNF138 n/a
9 TRCN0000417549 CAATCCTCAATCCTTACTAAA pLKO_005 904 CDS 100% 13.200 7.920 N ZNF138 n/a
10 TRCN0000012920 CCCTTACTAAACATCAGATAA pLKO.1 1082 CDS 100% 13.200 7.920 N ZNF138 n/a
11 TRCN0000422662 TTCACGCCTAACTCAACATAA pLKO_005 741 CDS 100% 13.200 7.920 N ZNF138 n/a
12 TRCN0000420316 AGAATGTGACAAATCACTTTG pLKO_005 714 CDS 100% 10.800 6.480 N ZNF138 n/a
13 TRCN0000012918 CCTCAACTCTTATTACACATA pLKO.1 1496 3UTR 100% 4.950 2.970 N ZNF138 n/a
14 TRCN0000428574 CTGAAGAGAAACCCTACAAAT pLKO_005 1025 CDS 100% 13.200 6.600 Y ZNF138 n/a
15 TRCN0000107875 CACTTGATTGTAGGTAAGATA pLKO.1 2410 3UTR 100% 5.625 2.813 Y ZNF254 n/a
16 TRCN0000017702 CCCTGGAATATGAAGAGACAT pLKO.1 367 5UTR 100% 4.950 2.475 Y ZNF430 n/a
17 TRCN0000019457 CCTCACACCTTACTAGACATA pLKO.1 992 CDS 100% 4.950 2.475 Y ZNF681 n/a
18 TRCN0000018502 GATGTTAGAGAACTACAGAAA pLKO.1 296 5UTR 100% 4.950 2.475 Y ZNF493 n/a
19 TRCN0000158848 GAGAAACCTTACAAATGTGAT pLKO.1 1702 3UTR 100% 4.950 2.475 Y ZNF28 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001367572.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11235 pDONR223 100% 65.6% 65.6% None 1_270del n/a
2 ccsbBroad304_11235 pLX_304 0% 65.6% 65.6% V5 1_270del n/a
3 TRCN0000481650 TTTATCAAAGGAATCCCCAATTTT pLX_317 100% 65.6% 65.6% V5 1_270del n/a
4 ccsbBroadEn_11550 pDONR223 100% 43.9% 38.2% None (many diffs) n/a
5 ccsbBroad304_11550 pLX_304 0% 43.9% 38.2% V5 (many diffs) n/a
6 ccsbBroadEn_11232 pDONR223 100% 41.2% 33.7% None (many diffs) n/a
7 ccsbBroad304_11232 pLX_304 0% 41.2% 33.7% V5 (many diffs) n/a
8 TRCN0000476048 TACTATCCCAATAGAAACACCTCC pLX_317 21.8% 41.2% 33.7% V5 (many diffs) n/a
9 TRCN0000477054 GAGCCAATTTATTAAACTTAACTA pLX_317 14.1% 27.7% 23.5% V5 (many diffs) n/a
10 ccsbBroadEn_15067 pDONR223 92.3% 27.7% 23.7% None (many diffs) n/a
11 ccsbBroad304_15067 pLX_304 0% 27.7% 23.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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