Transcript: Human NR_135191.2

Homo sapiens adaptor related protein complex 3 subunit mu 1 (AP3M1), transcript variant 6, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
AP3M1 (26985)
Length:
4911
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_135191.2
NBCI Gene record:
AP3M1 (26985)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_135191.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000065101 CCAACAATTCTACGCTCTGTT pLKO.1 486 3UTR 100% 4.950 6.930 N AP3M1 n/a
2 TRCN0000420255 GCCAGTCTGTCTGTGATTATT pLKO_005 151 3UTR 100% 15.000 10.500 N AP3M1 n/a
3 TRCN0000430284 ATGGAAATTTCCGACTCATAT pLKO_005 838 3UTR 100% 13.200 9.240 N AP3M1 n/a
4 TRCN0000421847 GATAATGTGGTCATAGTATAT pLKO_005 387 3UTR 100% 13.200 9.240 N AP3M1 n/a
5 TRCN0000111662 GCTTGCTATTTCAGGCTTAAA pLKO.1 1242 3UTR 100% 13.200 9.240 N Ap3m1 n/a
6 TRCN0000305487 GGATAATGTGGTCATAGTATA pLKO_005 386 3UTR 100% 13.200 9.240 N Ap3m1 n/a
7 TRCN0000417059 TGGCTACCGAATCTAACATTT pLKO_005 445 3UTR 100% 13.200 9.240 N AP3M1 n/a
8 TRCN0000425879 AGCTACACACCGCTAACAAAG pLKO_005 1469 3UTR 100% 10.800 7.560 N AP3M1 n/a
9 TRCN0000065099 GCGGCAGATTTGATATAACAA pLKO.1 940 3UTR 100% 5.625 3.938 N AP3M1 n/a
10 TRCN0000065100 CCGAGCCTCAACATACAGTTT pLKO.1 1210 3UTR 100% 4.950 3.465 N AP3M1 n/a
11 TRCN0000065102 CGGTGACATATTTCTAGAGAA pLKO.1 110 3UTR 100% 4.950 3.465 N AP3M1 n/a
12 TRCN0000065098 GCCTCATTAGACATCAAGAAA pLKO.1 2698 3UTR 100% 5.625 3.375 N AP3M1 n/a
13 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 4368 3UTR 100% 4.950 2.475 Y CFLAR n/a
14 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 4368 3UTR 100% 4.950 2.475 Y C19orf31 n/a
15 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 4407 3UTR 100% 4.050 2.025 Y P3H4 n/a
16 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 4407 3UTR 100% 4.050 2.025 Y ORAI2 n/a
17 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 4407 3UTR 100% 4.050 2.025 Y P3H4 n/a
18 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 4534 3UTR 100% 10.800 5.400 Y SMIM11A n/a
19 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 4366 3UTR 100% 4.950 2.475 Y ERN2 n/a
20 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 4366 3UTR 100% 4.950 2.475 Y P3H4 n/a
21 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 4366 3UTR 100% 4.950 2.475 Y P3H4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_135191.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02978 pDONR223 100% 25.5% None 1_77del;1089_1110del;1354_4911del n/a
2 ccsbBroad304_02978 pLX_304 0% 25.5% V5 1_77del;1089_1110del;1354_4911del n/a
3 TRCN0000492244 GCGCCTCCCCGGCGTCCGTGGCCA pLX_317 30.7% 25.5% V5 1_77del;1089_1110del;1354_4911del n/a
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