Transcript: Human NR_130149.2

Homo sapiens serine protease 2 (PRSS2), transcript variant 3, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
PRSS2 (5645)
Length:
735
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_130149.2
NBCI Gene record:
PRSS2 (5645)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_130149.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000046736 TCTGAGTTCTGGTGCCGACTA pLKO.1 380 3UTR 100% 4.050 3.240 N PRSS2 n/a
2 TRCN0000046737 CCGGACTCTGGACAATGACAT pLKO.1 242 3UTR 100% 4.950 3.465 N PRSS2 n/a
3 TRCN0000046734 CCCAAATACAACAGCCGGACT pLKO.1 228 3UTR 100% 2.160 1.512 N PRSS2 n/a
4 TRCN0000046733 CCCTGGAAAGATTACCAACAA pLKO.1 464 3UTR 100% 4.950 2.475 Y PRSS2 n/a
5 TRCN0000373302 CCTGGAAAGATTACCAACAGC pLKO_005 465 3UTR 100% 2.640 1.320 Y PRSS3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_130149.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06785 pDONR223 100% 82% None (many diffs) n/a
2 ccsbBroad304_06785 pLX_304 0% 82% V5 (many diffs) n/a
3 TRCN0000470572 ATGCAGTTCGTCAATATCTATCTT pLX_317 55.8% 82% V5 (many diffs) n/a
4 ccsbBroadEn_11061 pDONR223 100% 79.4% None 1_13del;52_53ins74;657_735del n/a
5 ccsbBroad304_11061 pLX_304 0% 79.4% V5 1_13del;52_53ins74;657_735del n/a
6 TRCN0000479459 CAACACTCTTAGGGCCAGCTAGCC pLX_317 41.3% 79.4% V5 1_13del;52_53ins74;657_735del n/a
7 TRCN0000489890 CTGGCACTGACCGGGTAGTTTCTA pLX_317 63.8% 79.4% V5 (not translated due to prior stop codon) 1_13del;52_53ins74;657_735del n/a
8 ccsbBroadEn_06784 pDONR223 100% 76% None (many diffs) n/a
9 ccsbBroad304_06784 pLX_304 0% 76% V5 (many diffs) n/a
10 TRCN0000477222 GTACCTAGGACCTAGTCATAGTTG pLX_317 46.7% 76% V5 (many diffs) n/a
11 ccsbBroadEn_11062 pDONR223 100% 75.6% None (many diffs) n/a
12 ccsbBroad304_11062 pLX_304 0% 75.6% V5 (many diffs) n/a
13 TRCN0000469423 GGACTTCGACTGTAAGCTACCGGG pLX_317 33.5% 75.6% V5 (many diffs) n/a
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