Transcript: Human NM_001308112.2

Homo sapiens kelch like family member 28 (KLHL28), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
KLHL28 (54813)
Length:
7019
CDS:
558..2315

Additional Resources:

NCBI RefSeq record:
NM_001308112.2
NBCI Gene record:
KLHL28 (54813)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001308112.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000145580 CAAATGTGAACTCTCGGAAAT pLKO.1 2316 CDS 100% 10.800 15.120 N KLHL28 n/a
2 TRCN0000144111 CCTCGCTATGAATTTGGAATA pLKO.1 1557 CDS 100% 10.800 15.120 N KLHL28 n/a
3 TRCN0000141463 CATGATATACTGTCGCTGCAA pLKO.1 2273 CDS 100% 2.640 3.696 N KLHL28 n/a
4 TRCN0000140525 GTACTGCATGAACATCCGGAT pLKO.1 2355 3UTR 100% 2.160 3.024 N KLHL28 n/a
5 TRCN0000142103 GCTATGTTCACTGGAAACCTT pLKO.1 783 CDS 100% 3.000 2.400 N KLHL28 n/a
6 TRCN0000144659 GCTGGAATCCTGATACAAATA pLKO.1 1672 CDS 100% 13.200 9.240 N KLHL28 n/a
7 TRCN0000419995 TGGTGCTGCTGTAATCGATAA pLKO_005 2156 CDS 100% 10.800 7.560 N KLHL28 n/a
8 TRCN0000141219 CAGCCCGTATTTCAAAGCTAT pLKO.1 767 CDS 100% 4.950 3.465 N KLHL28 n/a
9 TRCN0000144586 GACCAATGAAAGAACCTAGAA pLKO.1 2128 CDS 100% 4.950 3.465 N KLHL28 n/a
10 TRCN0000179309 GCTTGCTAACTTAACCCACTT pLKO.1 626 CDS 100% 4.050 2.835 N Klhl28 n/a
11 TRCN0000419389 GAGCGTTATGATCCAAGTAAG pLKO_005 1947 CDS 100% 10.800 6.480 N KLHL28 n/a
12 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 4555 3UTR 100% 4.950 2.475 Y ERAP2 n/a
13 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 4556 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001308112.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03462 pDONR223 100% 97.6% 97.6% None 1_42del n/a
2 ccsbBroad304_03462 pLX_304 0% 97.6% 97.6% V5 1_42del n/a
3 TRCN0000466093 ACATGTGCGCCCTATACTCCACAG pLX_317 22.9% 97.6% 97.6% V5 1_42del n/a
4 ccsbBroadEn_15871 pDONR223 0% 86.7% 86.1% None (many diffs) n/a
5 ccsbBroad304_15871 pLX_304 0% 86.7% 86.1% V5 (many diffs) n/a
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