Transcript: Human XM_005268284.5

PREDICTED: Homo sapiens golgin A8 family member M (GOLGA8M), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GOLGA8M (653720)
Length:
4035
CDS:
754..2790

Additional Resources:

NCBI RefSeq record:
XM_005268284.5
NBCI Gene record:
GOLGA8M (653720)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005268284.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000151320 GCAGGAGATTTGCACATTAAA pLKO.1 1671 CDS 100% 15.000 7.500 Y GOLGA8IP n/a
2 TRCN0000153447 CGCAGGAGATTTGCACATTAA pLKO.1 1670 CDS 100% 13.200 6.600 Y GOLGA8IP n/a
3 TRCN0000162932 GCAGTTGGAGCAGCAAGTAAA pLKO.1 2046 CDS 100% 13.200 6.600 Y GOLGA8B n/a
4 TRCN0000269218 CAGTTGGAGCAGCAAGTAAAG pLKO_005 2047 CDS 100% 10.800 5.400 Y GOLGA6L9 n/a
5 TRCN0000153167 GCCTATGTTCTGCTGTTGTTT pLKO.1 3197 3UTR 100% 5.625 2.813 Y GOLGA8G n/a
6 TRCN0000151499 CCAGACATTGAACATACAGAA pLKO.1 1296 CDS 100% 4.950 2.475 Y GOLGA8IP n/a
7 TRCN0000152586 GACACAGTTGAAGGAGTCATT pLKO.1 1563 CDS 100% 4.950 2.475 Y GOLGA8IP n/a
8 TRCN0000153104 GAGTGTTCTCTCTGATGTCAT pLKO.1 1434 CDS 100% 4.950 2.475 Y GOLGA8IP n/a
9 TRCN0000153085 GCAACATTCATTGCAGCGTAA pLKO.1 1401 CDS 100% 4.050 2.025 Y GOLGA8IP n/a
10 TRCN0000154490 GCAGCAAGTAAAGGAGCTACA pLKO.1 2055 CDS 100% 4.050 2.025 Y GOLGA8IP n/a
11 TRCN0000157310 CAGCGCATAAGTCTCCTGAAT pLKO.1 1879 CDS 100% 4.950 2.475 Y GOLGA8G n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005268284.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16170 pDONR223 0% 56.3% 51.3% None (many diffs) n/a
2 ccsbBroad304_16170 pLX_304 0% 56.3% 51.3% V5 (many diffs) n/a
3 ccsbBroadEn_10341 pDONR223 100% 55.9% 50.5% None (many diffs) n/a
4 ccsbBroad304_10341 pLX_304 0% 55.9% 50.5% V5 (many diffs) n/a
5 TRCN0000476380 AGTGATAACGTTCAAGGTCGCGGA pLX_317 28.7% 55.9% 50.5% V5 (many diffs) n/a
6 ccsbBroadEn_16172 pDONR223 0% 37.1% 33.5% None (many diffs) n/a
7 ccsbBroad304_16172 pLX_304 0% 37.1% 33.5% V5 (many diffs) n/a
8 TRCN0000480289 TACTAACTTATTTTTTGACAAACC pLX_317 100% 8.6% 8.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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