Transcript: Mouse XM_006508801.2

PREDICTED: Mus musculus tubulin, gamma complex associated protein 3 (Tubgcp3), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Tubgcp3 (259279)
Length:
3514
CDS:
1162..2655

Additional Resources:

NCBI RefSeq record:
XM_006508801.2
NBCI Gene record:
Tubgcp3 (259279)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006508801.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000434508 CCGTGACGCTGCGGATTTATT pLKO_005 1479 CDS 100% 15.000 21.000 N Tubgcp3 n/a
2 TRCN0000091981 GCTGCTCTTGTGAGAGATATT pLKO.1 806 5UTR 100% 13.200 10.560 N Tubgcp3 n/a
3 TRCN0000091978 GCATTCTCTGTGAGACAGTTT pLKO.1 2983 3UTR 100% 4.950 3.960 N Tubgcp3 n/a
4 TRCN0000435606 AGGAGTCTGGACCGCTCATTT pLKO_005 995 5UTR 100% 13.200 9.240 N Tubgcp3 n/a
5 TRCN0000091982 CCACTACCTGAGAGTGTTTAA pLKO.1 1905 CDS 100% 13.200 9.240 N Tubgcp3 n/a
6 TRCN0000091980 GAGGACACTTACCACGAATTT pLKO.1 1249 CDS 100% 13.200 9.240 N Tubgcp3 n/a
7 TRCN0000424361 GTCTATGGCACGACAAGTATA pLKO_005 1304 CDS 100% 13.200 9.240 N Tubgcp3 n/a
8 TRCN0000091979 GCCGTGTTTGACCAAATCATT pLKO.1 2260 CDS 100% 5.625 3.938 N Tubgcp3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006508801.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11497 pDONR223 100% 39.1% 43.3% None (many diffs) n/a
2 ccsbBroad304_11497 pLX_304 0% 39.1% 43.3% V5 (many diffs) n/a
3 TRCN0000479745 TACCAATGGACGCTCGAGTAGTCT pLX_317 14.4% 39.1% 43.3% V5 (many diffs) n/a
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