Construct: ORF ccsbBroad304_11497
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004641.1_s304c1, BRDN0000410398
- Derived from:
- ccsbBroadEn_11497
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBGCP3 (10426)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11497
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | NM_001286278.2 | 100% | 100% | |
| 2 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | XM_005268293.2 | 99.1% | 99% | (many diffs) |
| 3 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | NM_006322.6 | 90.6% | 89.9% | (many diffs) |
| 4 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | NM_001286277.2 | 89.5% | 88.8% | (many diffs) |
| 5 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | XM_011537462.2 | 79.6% | 78.9% | (many diffs) |
| 6 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | XM_017020323.2 | 65.4% | 64.7% | (many diffs) |
| 7 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | XR_001749455.2 | 60.1% | (many diffs) | |
| 8 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | NM_001286279.1 | 50.9% | 48.9% | (many diffs) |
| 9 | mouse | 259279 | Tubgcp3 | tubulin, gamma complex asso... | NM_198031.1 | 77.2% | 84.2% | (many diffs) |
| 10 | mouse | 259279 | Tubgcp3 | tubulin, gamma complex asso... | XM_006508798.1 | 75.2% | 81.9% | (many diffs) |
| 11 | mouse | 259279 | Tubgcp3 | tubulin, gamma complex asso... | XM_011242063.1 | 63.4% | 65.6% | (many diffs) |
| 12 | mouse | 259279 | Tubgcp3 | tubulin, gamma complex asso... | XM_006508800.1 | 61.4% | 65.2% | (many diffs) |
| 13 | mouse | 259279 | Tubgcp3 | tubulin, gamma complex asso... | XM_011242062.1 | 51% | 56.5% | (many diffs) |
| 14 | mouse | 259279 | Tubgcp3 | tubulin, gamma complex asso... | XM_006508801.2 | 39.1% | 43.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2538
- ORF length:
- 2472
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gaccccggac cagaagtcgc cgaacgttct gctgcagaac ctgtgctgca 121 ggatcctggg caggagcgaa gctgatgtag cccagcagtt ccagtatgct gtgcgggtga 181 ttggcagcaa cttcgcccca actgttgaaa gagatgaatt tttagtagct gaaaaaatca 241 agaaagagct tattcgacaa cgaagagaag cagatgctgc attattttca gaactccaca 301 gaaaacttca ttcacaggga gttttgaaaa ataaatggtc aatactctac ctcttgctga 361 gcctcagtga ggacccacgc aggcagccaa gcaaggtttc tagctatgct acgttatttg 421 ctcaggcctt accaagagat gcccactcaa ccccttacta ctatgccagg cctcagaccc 481 ttcccctgag ctaccaagat cggagtgccc agtcagccca gagctccggc agcgtgggca 541 gcagtggcat cagcagcatt ggcctgtgtg ccctcagtgg ccccgcgcct gcgccacaat 601 ctctcctccc aggacagtct aatcaagctc caggagtagg agattgcctt cgacagcagt 661 tggggtcacg actcgcatgg actttaactg caaatcagcc ttcttcacaa gccactacct 721 caaaaggtgt ccccagtgct gtgtctcgca acatgacaag gtccaggaga gaaggggata 781 cgggtggtac tatggaaatt acagaagcag ctctggtaag ggacattttg tacgtctttc 841 agggcataga tggcaaaaac atcaaaatga acaacactga aaattgttac aaagtagaag 901 gaaaggcaaa tctaagtagg tctttgagag acacagcagt caggctttct gagttgggat 961 ggttgcataa taaaatcaga agatacacgg accagaggag cctggaccgc tcattcggac 1021 tcgtcgggca gagcttttgt gctgccttgc accaggaact cagagaatac tatcgattgc 1081 tctctgtttt acattctcag ctacaactag aggatgacca gggtgtgaat ttgggacttg 1141 agagtagttt aacacttcgg cgcctcctgg tttggaccta tgatcccaaa atacgactga 1201 agacccttgc ggccctagtg gaccactgcc aaggaaggaa aggaggtgag ctggcctcag 1261 ctgtccacgc ctacacaaaa acaggagacc cgtacatgcg gtctctggtg cagcacatcc 1321 tcagcctcgt gtctcatcct gttttgagct tcctgtaccg ctggatatat gatggggagc 1381 ttgaggacac ttaccacgaa ttttttgtag catcagatcc aacagttaaa acagatcgac 1441 tgtggcacga caagtatact ttgaggaaat cgatgattcc ttcgtttatg acgatggatc 1501 agtctaggaa ggtccttttg ataggaaaat caataaattt cttgcaccaa gtttgtcatg 1561 atcagactcc cactacaaag atgatagctg tgaccaagtc tgcagagtca ccccaggacg 1621 ctgcagacct attcacagac ttggaaaatg catttcaggg gaagattgat gctgcttatt 1681 ttgagaccag caaatacctg ttggatgttc tcaataaaaa gtacagcttg ctggaccaca 1741 tgcaggcaat gaggcggtac ctgcttcttg gtcaaggaga ctttataagg cacttaatgg 1801 acttgctaaa accagaactt gtccgtccag ctacgacttt gtatcagcat aacttgactg 1861 gaattctaga aaccgctgtc agagccacca acgcacagtt tgacagtcct gagatcctgc 1921 gaaggctgga cgtgcggctg ctggaggtct ctccaggtga cactggatgg gatgtcttca 1981 gcctcgatta tcatgttgac ggaccaattg caactgtgtt tactcgagaa tgtatgagcc 2041 actacctaag agtatttaac ttcctctgga gggcgaagcg gatggaatac atcctcactg 2101 acatacggaa gggacacatg tgcaatgcaa agctcctgag aaacatgcca gagttctccg 2161 gggtgctgca ccagtgtcac attttggcct ctgagatggt ccatttcatt catcagatgc 2221 agtattacat cacatttgag gtgcttgaat gttcttggga tgagctttgg aacaaagtcc 2281 agcaggccca ggatttggat cacatcattg ctgcacacga ggtgttctta gacaccatca 2341 tctcccgctg cctgctggac agtgactcca gggcactttt aaatcaactt agagctgtgt 2401 ttgatcaaat tattgaactt cagaatgctc aagatgcaat atacagagct gctctggaag 2461 aattgcagag acgattacag tttgaagaga aaaagaaaca gcgtgaaatt gaggttgaga 2521 tgtgcctata ctgcgtttgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 2581 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2641 agtcgacaat caacctctgg atta