Transcript: Mouse XM_006515166.2

PREDICTED: Mus musculus basic leucine zipper and W2 domains 2 (Bzw2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Bzw2 (66912)
Length:
2591
CDS:
165..1328

Additional Resources:

NCBI RefSeq record:
XM_006515166.2
NBCI Gene record:
Bzw2 (66912)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006515166.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000437591 AGGTGGTGCTTTACGTCAAAG pLKO_005 985 CDS 100% 10.800 15.120 N Bzw2 n/a
2 TRCN0000096855 CGATTAGATTACCGTCGCTAT pLKO.1 336 CDS 100% 4.050 5.670 N Bzw2 n/a
3 TRCN0000418953 AGGAATACTGCTACGACAACA pLKO_005 1204 CDS 100% 4.950 3.960 N Bzw2 n/a
4 TRCN0000139465 GATGACCAACCACTGTGTGTT pLKO.1 440 CDS 100% 4.950 3.960 N BZW2 n/a
5 TRCN0000096857 GCATCATGAATGCGGTGGAAT pLKO.1 1063 CDS 100% 4.950 3.960 N Bzw2 n/a
6 TRCN0000096856 CCGAAACTATGCTCAGGTCTT pLKO.1 488 CDS 100% 4.050 3.240 N Bzw2 n/a
7 TRCN0000140053 CCGAAACTATGCTCAGGTCTT pLKO.1 488 CDS 100% 4.050 3.240 N BZW2 n/a
8 TRCN0000086958 CCTGGGATGTATGCTTGTTAT pLKO.1 1548 3UTR 100% 13.200 9.240 N LOC433915 n/a
9 TRCN0000092213 CTGGGATGTATGCTTGTTATA pLKO.1 1549 3UTR 100% 13.200 9.240 N LOC433861 n/a
10 TRCN0000414051 ACCTTGAAGCTGTAGCCAAAT pLKO_005 295 CDS 100% 10.800 7.560 N Bzw2 n/a
11 TRCN0000430118 CTCCTCTTCCTTAAAGCATTT pLKO_005 573 CDS 100% 10.800 7.560 N Bzw2 n/a
12 TRCN0000419501 CTCATCAGGAGATACAAGTAT pLKO_005 516 CDS 100% 5.625 3.938 N Bzw2 n/a
13 TRCN0000427477 TGTGGATCATTTCGCTAAGTA pLKO_005 842 CDS 100% 5.625 3.938 N Bzw2 n/a
14 TRCN0000087039 AGGACCAGATGGAACCTCTTT pLKO.1 1271 CDS 100% 4.950 3.465 N LOC433911 n/a
15 TRCN0000092356 GATGAGAGATCCTTTCGGTAT pLKO.1 1395 3UTR 100% 4.050 2.835 N LOC434560 n/a
16 TRCN0000087516 TCGGTATTCCAGAAGCCGATA pLKO.1 1409 3UTR 100% 4.050 2.835 N LOC434774 n/a
17 TRCN0000092355 GATCCCACAAGCACAAAGGAT pLKO.1 1366 3UTR 100% 3.000 2.100 N LOC434560 n/a
18 TRCN0000092178 CCAATGCTGTTTGTCACCCAA pLKO.1 2114 3UTR 100% 2.640 1.848 N LOC432446 n/a
19 TRCN0000087513 CGTGGATGAGAGAGAGTATAA pLKO.1 2421 3UTR 100% 13.200 7.920 N LOC434774 n/a
20 TRCN0000092558 GCTCGACATGACATTCAAATT pLKO.1 1433 3UTR 100% 13.200 7.920 N LOC434815 n/a
21 TRCN0000087038 CCCTGGGATGTATGCTTGTTA pLKO.1 1547 3UTR 100% 5.625 3.375 N LOC433911 n/a
22 TRCN0000087066 AGCCGAGATGAGAGATCCTTT pLKO.1 1389 3UTR 100% 4.950 2.970 N LOC432823 n/a
23 TRCN0000087368 GCTTGTTATAGTGTGTGTGTT pLKO.1 1560 3UTR 100% 4.950 2.970 N LOC433663 n/a
24 TRCN0000087308 CGGGACAATCTCCTCAGATTT pLKO.1 1664 3UTR 100% 1.320 0.792 N LOC434331 n/a
25 TRCN0000086928 GCACCTGACTTCCTCATATAA pLKO.1 2273 3UTR 100% 15.000 7.500 Y LOC432510 n/a
26 TRCN0000087063 CCAACCTAAGACAGGGATCAA pLKO.1 2092 3UTR 100% 4.950 2.475 Y LOC432823 n/a
27 TRCN0000087311 CCTGTGGATGTGGTGGATGAT pLKO.1 1348 3UTR 100% 4.950 2.475 Y LOC434331 n/a
28 TRCN0000087578 GAATCCAATTTGGTGGAGATT pLKO.1 2339 3UTR 100% 4.950 2.475 Y LOC434071 n/a
29 TRCN0000086718 TGCAAGAATGAGGCTCCTGAT pLKO.1 1318 CDS 100% 4.050 2.025 Y LOC233467 n/a
30 TRCN0000092318 CTTGTTATAGTGTGTGTGTTT pLKO.1 1561 3UTR 100% 4.950 2.970 N LOC433995 n/a
31 TRCN0000087349 CCCTGTGGATGTGGTGGATAT pLKO.1 1347 3UTR 100% 10.800 5.400 Y LOC434680 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006515166.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03047 pDONR223 100% 83.4% 85.6% None (many diffs) n/a
2 ccsbBroad304_03047 pLX_304 0% 83.4% 85.6% V5 (many diffs) n/a
3 TRCN0000473591 AGGATATTAACCGCGTTTGTCCAC pLX_317 33.5% 83.4% 85.6% V5 (many diffs) n/a
4 ccsbBroadEn_15799 pDONR223 0% 83.3% 85.6% None (many diffs) n/a
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