Transcript: Human XM_006710423.2

PREDICTED: Homo sapiens coiled-coil and C2 domain containing 1B (CC2D1B), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CC2D1B (200014)
Length:
3381
CDS:
238..2688

Additional Resources:

NCBI RefSeq record:
XM_006710423.2
NBCI Gene record:
CC2D1B (200014)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006710423.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000168106 CGGAAAGTCAACTTTGCTGAA pLKO.1 1531 CDS 100% 4.050 3.240 N CC2D1B n/a
2 TRCN0000434529 AGGCACGGAAACTGCAGTATC pLKO_005 1859 CDS 100% 10.800 7.560 N CC2D1B n/a
3 TRCN0000418650 AGTTTGAGATCTTCCACAAAG pLKO_005 2447 CDS 100% 10.800 7.560 N CC2D1B n/a
4 TRCN0000172915 GCTGGAGAATGAGTGTGAGAT pLKO.1 2523 CDS 100% 4.950 3.465 N CC2D1B n/a
5 TRCN0000172971 GCCTCAAGTTCTAAGGAGTCA pLKO.1 1801 CDS 100% 2.640 1.584 N CC2D1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006710423.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14427 pDONR223 100% 56.2% 55.2% None (many diffs) n/a
2 ccsbBroad304_14427 pLX_304 0% 56.2% 55.2% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000471693 GACGGCAGGGCAGTCACCTAAGGA pLX_317 22.7% 56.2% 55.2% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_13380 pDONR223 100% 19.3% 19.3% None 1_1974del n/a
5 ccsbBroad304_13380 pLX_304 0% 19.3% 19.3% V5 1_1974del n/a
6 TRCN0000467342 ATAAAATCCCCCATTCACTACGTG pLX_317 88.2% 19.3% 19.3% V5 1_1974del n/a
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