Construct: ORF TRCN0000471693

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000144.1_s317c1
Derived from:
ccsbBroadEn_14427
DNA Barcode:
GACGGCAGGGCAGTCACCTAAGGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
CC2D1B (200014)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471693
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 200014 CC2D1B coiled-coil and C2 domain c... XM_017000559.1 96.4% 94.8% (many diffs)
2 human 200014 CC2D1B coiled-coil and C2 domain c... XM_024453857.1 96.4% 94.8% (many diffs)
3 human 200014 CC2D1B coiled-coil and C2 domain c... XM_005270593.2 95.4% 93.8% (many diffs)
4 human 200014 CC2D1B coiled-coil and C2 domain c... NM_001330585.2 61.5% 60.5% (many diffs)
5 human 200014 CC2D1B coiled-coil and C2 domain c... NM_032449.2 61.1% 60.1% (many diffs)
6 human 200014 CC2D1B coiled-coil and C2 domain c... XM_005270590.2 61.1% 60.1% (many diffs)
7 human 200014 CC2D1B coiled-coil and C2 domain c... XM_006710423.2 56.2% 55.2% (many diffs)
8 human 200014 CC2D1B coiled-coil and C2 domain c... XR_001737029.1 45.6% (many diffs)
9 human 200014 CC2D1B coiled-coil and C2 domain c... XR_001737027.1 20.7% (many diffs)
10 human 200014 CC2D1B coiled-coil and C2 domain c... XR_001737028.1 19% (many diffs)
11 human 200014 CC2D1B coiled-coil and C2 domain c... XR_002959657.1 18.6% (many diffs)
12 mouse 319965 Cc2d1b coiled-coil and C2 domain c... XM_006503154.2 74.9% 73.6% (many diffs)
13 mouse 319965 Cc2d1b coiled-coil and C2 domain c... XM_006503152.3 55.1% 54.1% (many diffs)
14 mouse 319965 Cc2d1b coiled-coil and C2 domain c... NM_177045.3 53% 52% (many diffs)
15 mouse 319965 Cc2d1b coiled-coil and C2 domain c... XR_376327.1 40.7% (many diffs)
16 mouse 319965 Cc2d1b coiled-coil and C2 domain c... XR_376326.1 39.8% (many diffs)
17 mouse 319965 Cc2d1b coiled-coil and C2 domain c... XR_376325.2 39.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1635
ORF length:
1569
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag gattgggaag agattcggtg ctgtcctgga ggccctggag aaggggcagc
121ccgtggatct gagtgccatg cccccggcac ctgaggatct gaagccccag caggcttctc
181aggctcccac agcaccctca gtcattcccc cagccgtgga gcgagtgcag ccagtgatgg
241cccctgacgt cccagcaacc ccagtggccc ctacagagtc acagacagtg ctggatgccc
301tgcagcagag gctgaacaag taccgcgagg cgggcatcca ggcccggagt ggtggggacg
361agcgcaaggc tcggatgcat gagcgcattg ccaagcaata tcaagatgct attcgagcac
421accgagcagg acggaaagtc aactttgctg aattgcctgt tcctccagga tttcccccca
481tccctggcct ggagtccact atgggtgttg aggaggacgc agtggcagcg acattggcag
541ctgcagagaa actggcctct gcagaggatt cagccccggc tgataaagac gaggacgagg
601gtgagccccc agcacaggcc ccagtggcca agaaacctgc acggcccaca gtcccttcat
661cccagcgcct gcctgagccc agggcctcaa gttctaagga gtcaccgagt ccatctgtgc
721gggagcagct ggcactgctg gaggcacgga aactgcagta tcagcgggca gccctgcagg
781ccaagcgcag ccaggacctg gagcaggcca aagcctatct gcgggtagcc aaatggcttg
841aggctcagat catccaggcc cgatctggca gacctgttga tctgtccaag gtgccttcgc
901ccttgacgga tgaggagggt gacttcatcc tcatccacca tgaggacctg cgactctccc
961agaaggcgga ggaggtgtat gcccagctgc aaaaaatgct tctggagcaa caagagaagt
1021gcctgctgtt ctccaagcag ttcatgcacc agggcaacgt ggctgagacc acccgatttg
1081agaagcttgc tcaggaccgc aagaaacagc tggagatcct gcagctggcc caggctcagg
1141gcctcgaccc tcccacccac cactttgagt tgaagacatt ccagactgtg aggatcttct
1201cagaactcaa cagcacagaa atgcatctga tcattgtccg gggaatgaac ctcccagccc
1261ctccaggggt gactcccgat gacctggatg cttttgtgcg gtttGAGTTT CACTACCCTA
1321ACTCGGACCA GGCTCAAAAA AGCAAAACAG CTGTGGTGAA GAACACAAAC TCTCCAGAAT
1381TTGATCAACT CTTCAAACTA AACATCAACC GAAACCACCG GGGCTTCAAG AGGGTGATCC
1441AGAGCAAAGG CATCAAGTTT GAGATCTTCC ACAAAGGGTC CTTCTTCAGA AGCGACAAGC
1501TGGTTGGCAC AGCACACCTG AAACTGGAGC GGCTGGAGAA TGAGTGTGAG ATCAGAGAAA
1561TTGTGGAGGT CCTGGATGGA AGGAAGCCCA CCGGGGGGAA GCTGGAGGTG AAGCCGATGG
1621CCAGCACCAG GAGATGAGTC AGGCCGCGAC TGTGCAGGAG ATGCCCAACT TTCTTGTACA
1681AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1741AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1801AGGACGAGAC GGCAGGGCAG TCACCTAAGG AACGCGTTAA GTCgacaatc aacctctgga
1861ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)