Transcript: Human XM_011519185.3

PREDICTED: Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MRRF (92399)
Length:
3560
CDS:
312..980

Additional Resources:

NCBI RefSeq record:
XM_011519185.3
NBCI Gene record:
MRRF (92399)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011519185.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000045270 GCAATTATCTTGCAGCCTCTA pLKO.1 421 CDS 100% 4.050 3.240 N MRRF n/a
2 TRCN0000290438 GCAATTATCTTGCAGCCTCTA pLKO_005 421 CDS 100% 4.050 3.240 N MRRF n/a
3 TRCN0000045271 GCTGCCTTGGTTGAGGATATA pLKO.1 600 CDS 100% 13.200 9.240 N MRRF n/a
4 TRCN0000290508 GCTGCCTTGGTTGAGGATATA pLKO_005 600 CDS 100% 13.200 9.240 N MRRF n/a
5 TRCN0000248989 TGCAGCTATCAAGGCTATAAG pLKO_005 842 CDS 100% 13.200 9.240 N Mrrf n/a
6 TRCN0000045268 CACTGAAGACAGTGCATGAAA pLKO.1 463 CDS 100% 0.563 0.394 N MRRF n/a
7 TRCN0000290437 CACTGAAGACAGTGCATGAAA pLKO_005 463 CDS 100% 0.563 0.394 N MRRF n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011519185.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04579 pDONR223 100% 71% 62.1% None 1_63del;614_615ins160;666_667ins23 n/a
2 ccsbBroad304_04579 pLX_304 0% 71% 62.1% V5 1_63del;614_615ins160;666_667ins23 n/a
3 TRCN0000474938 TATGTATCACCGACCATACAATCA pLX_317 65.7% 71% 62.1% V5 1_63del;614_615ins160;666_667ins23 n/a
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