Construct: ORF TRCN0000474938
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006857.1_s317c1
- Derived from:
- ccsbBroadEn_04579
- DNA Barcode:
- TATGTATCACCGACCATACAATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRRF (92399)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474938
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92399 | MRRF | mitochondrial ribosome recy... | NM_001346341.2 | 100% | 100% | |
| 2 | human | 92399 | MRRF | mitochondrial ribosome recy... | NM_138777.5 | 100% | 100% | |
| 3 | human | 92399 | MRRF | mitochondrial ribosome recy... | NM_001346339.2 | 92.5% | 92.5% | 1_63del |
| 4 | human | 92399 | MRRF | mitochondrial ribosome recy... | XM_011519183.2 | 92.5% | 92.5% | 1_63del |
| 5 | human | 92399 | MRRF | mitochondrial ribosome recy... | NM_001346343.2 | 83.2% | 83.2% | 0_1ins132 |
| 6 | human | 92399 | MRRF | mitochondrial ribosome recy... | NM_001173512.2 | 80.1% | 80.1% | 182_183ins156 |
| 7 | human | 92399 | MRRF | mitochondrial ribosome recy... | NM_199177.4 | 76.7% | 66.9% | 551_552ins160;603_604ins23 |
| 8 | human | 92399 | MRRF | mitochondrial ribosome recy... | NM_001346345.2 | 76.3% | 57.8% | 459_460ins92;600_601ins94 |
| 9 | human | 92399 | MRRF | mitochondrial ribosome recy... | XM_011519185.3 | 71% | 62.1% | 1_63del;614_615ins160;666_667ins23 |
| 10 | human | 92399 | MRRF | mitochondrial ribosome recy... | XM_017015286.2 | 70.6% | 53.8% | 1_63del;522_523ins92;663_664ins94 |
| 11 | human | 92399 | MRRF | mitochondrial ribosome recy... | NM_001346347.2 | 65.2% | 65.2% | 0_1ins273 |
| 12 | human | 92399 | MRRF | mitochondrial ribosome recy... | XR_428543.4 | 34% | 1_374del;832_1126del;1456_2305del | |
| 13 | human | 92399 | MRRF | mitochondrial ribosome recy... | XR_002956824.1 | 31.3% | (many diffs) | |
| 14 | human | 92399 | MRRF | mitochondrial ribosome recy... | XR_002956825.1 | 30.6% | (many diffs) | |
| 15 | human | 92399 | MRRF | mitochondrial ribosome recy... | XR_001746416.2 | 20.6% | 1_374del;925_1014del;1251_3810del | |
| 16 | human | 92399 | MRRF | mitochondrial ribosome recy... | XR_001746417.2 | 17.6% | (many diffs) | |
| 17 | human | 92399 | MRRF | mitochondrial ribosome recy... | NR_144421.2 | 7.9% | 1_85del;543_837del;1167_9889del | |
| 18 | mouse | 67871 | Mrrf | mitochondrial ribosome recy... | NM_026422.2 | 86.7% | 85.1% | (many diffs) |
| 19 | mouse | 67871 | Mrrf | mitochondrial ribosome recy... | XM_017319228.1 | 69.9% | 69% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 852
- ORF length:
- 786
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cttgggatta aagtgcttcc gcatggtcca ccctaccttt cgcaattatc 121 ttgcagcctc tatcagaccc gtttcagaag ttacactgaa gacagtgcat gaaagacaac 181 atggccatag gcaatacatg gcctattcag ctgtaccagt ccgccatttt gctaccaaga 241 aagccaaagc caaagggaaa ggacagtccc aaaccagagt gaatattaat gctgccttgg 301 ttgaggatat aatcaacttg gaagaggtga atGAAGAAAT GAAGTCTGTG ATAGAAGCTC 361 TCAAGGATAA TTTCAATAAG ACTCTCAATA TAAGGACCTC ACCAGGATCC CTTGACAAGA 421 TTGCTGTGGT AACTGCTGAC GGGAAGCTTG CTTTAAACCA GATTAGCCAG ATCTCCATGA 481 AGTCGCCACA GCTGATTTTG GTGAATATGG CCAGCTTCCC AGAGTGTACA GCTGCAGCTA 541 TCAAGGCTAT AAGAGAAAGT GGAATGAATC TGAACCCAGA AGTGGAAGGG ACGCTAATTC 601 GGGTACCCAT TCCCCAAGTA ACCAGAGAGC ACAGAGAAAT GCTGGTGAAA CTGGCCAAAC 661 AGAACACCAA CAAGGCCAAA GACTCTTTAC GGAAGGTTCG CACCAACTCA ATGAACAAGC 721 TGAAGAAATC CAAGGATACA GTCTCAGAGG ACACCATTAG GCTAATAGAG AAACAGATCA 781 GCCAAATGGC CGATGACACA GTGGCAGAAC TGGACAGGCA TCTGGCAGTG AAGACCAAAG 841 AACTCCTTGG ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 901 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 961 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAT GTATCACCGA CCATACAATC 1021 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t