Construct: ORF ccsbBroad304_04579

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006857.1_s304c1, BRDN0000399775
Derived from:
ccsbBroadEn_04579
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MRRF (92399)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04579
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 92399 MRRF mitochondrial ribosome recy... NM_001346341.2 100% 100%
2 human 92399 MRRF mitochondrial ribosome recy... NM_138777.5 100% 100%
3 human 92399 MRRF mitochondrial ribosome recy... NM_001346339.2 92.5% 92.5% 1_63del
4 human 92399 MRRF mitochondrial ribosome recy... XM_011519183.2 92.5% 92.5% 1_63del
5 human 92399 MRRF mitochondrial ribosome recy... NM_001346343.2 83.2% 83.2% 0_1ins132
6 human 92399 MRRF mitochondrial ribosome recy... NM_001173512.2 80.1% 80.1% 182_183ins156
7 human 92399 MRRF mitochondrial ribosome recy... NM_199177.4 76.7% 66.9% 551_552ins160;603_604ins23
8 human 92399 MRRF mitochondrial ribosome recy... NM_001346345.2 76.3% 57.8% 459_460ins92;600_601ins94
9 human 92399 MRRF mitochondrial ribosome recy... XM_011519185.3 71% 62.1% 1_63del;614_615ins160;666_667ins23
10 human 92399 MRRF mitochondrial ribosome recy... XM_017015286.2 70.6% 53.8% 1_63del;522_523ins92;663_664ins94
11 human 92399 MRRF mitochondrial ribosome recy... NM_001346347.2 65.2% 65.2% 0_1ins273
12 human 92399 MRRF mitochondrial ribosome recy... XR_428543.4 34% 1_374del;832_1126del;1456_2305del
13 human 92399 MRRF mitochondrial ribosome recy... XR_002956824.1 31.3% (many diffs)
14 human 92399 MRRF mitochondrial ribosome recy... XR_002956825.1 30.6% (many diffs)
15 human 92399 MRRF mitochondrial ribosome recy... XR_001746416.2 20.6% 1_374del;925_1014del;1251_3810del
16 human 92399 MRRF mitochondrial ribosome recy... XR_001746417.2 17.6% (many diffs)
17 human 92399 MRRF mitochondrial ribosome recy... NR_144421.2 7.9% 1_85del;543_837del;1167_9889del
18 mouse 67871 Mrrf mitochondrial ribosome recy... NM_026422.2 86.7% 85.1% (many diffs)
19 mouse 67871 Mrrf mitochondrial ribosome recy... XM_017319228.1 69.9% 69% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
852
ORF length:
786
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cttgggatta aagtgcttcc gcatggtcca ccctaccttt cgcaattatc
121ttgcagcctc tatcagaccc gtttcagaag ttacactgaa gacagtgcat gaaagacaac
181atggccatag gcaatacatg gcctattcag ctgtaccagt ccgccatttt gctaccaaga
241aagccaaagc caaagggaaa ggacagtccc aaaccagagt gaatattaat gctgccttgg
301ttgaggatat aatcaacttg gaagaggtga atgaagaaat gaagtctgtg atagaagctc
361tcaaggataa tttcaataag actctcaata taaggacctc accaggatcc cttgacaaga
421ttgctgtggt aactgctgac gggaagcttg ctttaaacca gattagccag atctccatga
481agtcgccaca gctgattttg gtgaatatgg ccagcttccc agagtgtaca gctgcagcta
541tcaaggctat aagagaaagt ggaatgaatc tgaacccaga agtggaaggg acgctaattc
601gggtacccat tccccaagta accagagagc acagagaaat gctggtgaaa ctggccaaac
661agaacaccaa caaggccaaa gactctttac ggaaggttcg caccaactca atgaacaagc
721tgaagaaatc caaggataca gtctcagagg acaccattag gctaatagag aaacagatca
781gccaaatggc cgatgacaca gtggcagaac tggacaggca tctggcagtg aagaccaaag
841aactccttgg atgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
901tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
961caatcaacct ctggatta

Download FASTA (ORF) (Full)