Transcript: Human XM_017019412.2

PREDICTED: Homo sapiens TAO kinase 3 (TAOK3), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TAOK3 (51347)
Length:
5273
CDS:
1995..4208

Additional Resources:

NCBI RefSeq record:
XM_017019412.2
NBCI Gene record:
TAOK3 (51347)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017019412.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000273335 TAACTAGCTGCTGCATTATTT pLKO_005 4657 3UTR 100% 15.000 21.000 N TAOK3 n/a
2 TRCN0000242196 AGCGCTTTGCCACGATCAAAT pLKO_005 2809 CDS 100% 13.200 18.480 N Taok3 n/a
3 TRCN0000273361 AGCGCTTTGCCACGATCAAAT pLKO_005 2809 CDS 100% 13.200 18.480 N TAOK3 n/a
4 TRCN0000001527 GAGTACAATAAGAGGCGAGAA pLKO.1 3573 CDS 100% 4.050 3.240 N TAOK3 n/a
5 TRCN0000273336 ATGACGAAAGCACAATCAATT pLKO_005 2632 CDS 100% 13.200 9.240 N TAOK3 n/a
6 TRCN0000382396 GAGCGGATCTCCAAACATAAA pLKO_005 3249 CDS 100% 13.200 9.240 N TAOK3 n/a
7 TRCN0000380562 TGGACTAGCCTACCTACATTC pLKO_005 861 5UTR 100% 10.800 7.560 N TAOK3 n/a
8 TRCN0000196860 GCCGATCTATTCTACAAAGAT pLKO.1 496 5UTR 100% 5.625 3.938 N TAOK3 n/a
9 TRCN0000001525 CCGTCTCTTCTATTCACAGTA pLKO.1 4430 3UTR 100% 4.950 3.465 N TAOK3 n/a
10 TRCN0000001529 AGCGAGAGAATAAAGAACCTA pLKO.1 4083 CDS 100% 3.000 2.100 N TAOK3 n/a
11 TRCN0000001526 GCAGCAACCATTCCATTCCAA pLKO.1 2518 CDS 100% 3.000 2.100 N TAOK3 n/a
12 TRCN0000194986 CGTCACAGTATTGATGTGATT pLKO.1 4453 3UTR 100% 0.495 0.347 N TAOK3 n/a
13 TRCN0000273334 GGAACAGATGTCAGGTTATAA pLKO_005 2885 CDS 100% 15.000 9.000 N TAOK3 n/a
14 TRCN0000001528 GAAAGGCAAGAGCGAGAGATT pLKO.1 4107 CDS 100% 4.950 2.970 N TAOK3 n/a
15 TRCN0000273337 GAAAGGCAAGAGCGAGAGATT pLKO_005 4107 CDS 100% 4.950 2.970 N TAOK3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017019412.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15065 pDONR223 0% 80.2% 80.2% None 0_1ins510;685_711del n/a
2 ccsbBroad304_15065 pLX_304 0% 80.2% 80.2% V5 0_1ins510;685_711del n/a
3 TRCN0000465631 GATCACGGGGCAACCCTTCGATAC pLX_317 13.2% 80.2% 80.2% V5 0_1ins510;685_711del n/a
4 TRCN0000487785 CCTCCAGGGTGACACTCCAAACGT pLX_317 10.6% 80.2% 80.1% V5 (not translated due to prior stop codon) 0_1ins510;685_711del;748G>A n/a
5 TRCN0000488252 CCATAATCCTTCGCATTACGCTCA pLX_317 13.1% 80.1% 80% V5 (many diffs) n/a
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