Construct: ORF ccsbBroad304_15307

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018869.1_s304c1, BRDN0000459353
Derived from:
ccsbBroadEn_15307
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
NEK5 (341676)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15307
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 341676 NEK5 NIMA related kinase 5 NM_199289.3 95.6% 36% (many diffs)
2 human 341676 NEK5 NIMA related kinase 5 XM_017020555.1 89.7% 33.7% (many diffs)
3 human 341676 NEK5 NIMA related kinase 5 XM_017020557.1 86.8% 32.6% (many diffs)
4 human 341676 NEK5 NIMA related kinase 5 XM_017020556.1 83.3% 34.5% (many diffs)
5 human 341676 NEK5 NIMA related kinase 5 XM_011535066.2 81.6% 36.5% (many diffs)
6 human 341676 NEK5 NIMA related kinase 5 NM_001365552.1 80.6% 30.6% (many diffs)
7 human 341676 NEK5 NIMA related kinase 5 XR_941575.2 74.4% (many diffs)
8 human 341676 NEK5 NIMA related kinase 5 XM_006719808.4 67.9% 42.6% (many diffs)
9 human 341676 NEK5 NIMA related kinase 5 XR_941574.2 64.4% (many diffs)
10 human 341676 NEK5 NIMA related kinase 5 XM_011535068.2 53.8% 52.7% (many diffs)
11 human 341676 NEK5 NIMA related kinase 5 XR_001749542.1 48.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
861
ORF length:
792
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggataagtac gatgtgatta aggccatcgg gcaaggtgcc ttcgggaaag
121catacttagc taaagggaaa tcagatagca agcactgtgt cataaaagag atcaattttg
181aaaagatgcc catacaagaa aaagaagctt caaagaaaga agtgattctt ctggaaaaga
241tgaaacatcc caacattgta gccttcttca attcatttca agagaatggc aggctgttta
301ttgtaatgga atattgtgat ggaggggatc tcatgaaaag gatcaataga caacggggtg
361tgttatttag tgaagatcag atcctcggtt ggtttgtaca gatttctcta ggactaaaac
421atattcatga caggaagata ttacacaggg acataaaagc tcagaacatt tttcttagca
481agaacggaat ggtggcaaag cttggggact ttggtatagc aagagtcctg aataattcca
541tggaacttgc tcgaacttgt attggaacac cttactacct gtccccagag atctgtcaga
601ataaacccta caacaataaa acggatattt ggtctcttgg ctgtgtctta tatgagctct
661gcacacttaa acatcctttt gagggtaaca acttacagca gctggttctg aagatttgtc
721aagcacattt tgccccaata tctccggggt tttctcgtga gctccattcc ttgatatctc
781agctctttca agtatctctc gagaccgaca tcataaattc cattttgaaa gcccttttag
841agaatcttat tcccaatatt tgactcctgg aggtcattca ggaggaattc agtcacatgc
901ttatatgcag agcaggagcg ccagcttctc gacatgctgg gaaggtggtc cagaagtgta
961aaatacaaaa agtgagattc cagggaaagt gcccaccaag atcaaggata tctgtgccaa
1021ttaaaaggaa tgctatattg catagaaatg aatggagacc accagctgga gcccagaagg
1081ccagatctat aaaaatgata gaaagaccca aaattgctgc tgtctgtgga cattatgatt
1141attattatgc tcaacttgat atgctgagga ggagagccca caaaccaagt tatcacccta
1201ttcctcaaga aaatactgga gttgaggatt acggtcagga aacgaggcat ggtccatccc
1261caagtcaatg gcctgctgag taccttcaga gaaaatttga aggtccacaa tataagtttg
1321aaagtggaga agcaattggg tcttcgtcca tctcttgccg agccaaatta caaccaagag
1381acaagagcta gnaagtaatg gagaagagcc tagattccag gagctgccat ttaggaaaaa
1441cgaaatgaag gaacaggaat attggaagca gttagaggaa atacgccaac agtaccacaa
1501tgacatgaaa gaaattagaa agaagatggg gagagaacca gaggagaact caaaaataag
1561tcataaaacc tatttggtga agaagagtaa cctgcctgtc catcaagatg catctgaggg
1621agaagcacct gtgcaggaca ttgaaaaaga cttgaaacaa atgaggcttc agaacacaaa
1681ggaaagtaaa aatccagaac agaaatataa agctaagaag ggggtaaaat ttgaaattaa
1741tttagacaaa tgtatttctg atgaaaacat cctccaagag gaagaggcaa tggatatacc
1801aaatgaaact ttgacctttg aggatggcat gaagtttaag gaatatgaat gtgtaaagga
1861gcatggagat tatacagaca aagcatttga aaaacttcac tgcccagaag cagggttttc
1921cacgcagact gtagctgctg tgggaaacag gaggcagtgg gatggaggag cgcctcagac
1981tctgctgcag atgatggcag tggccgacat cacctccacc tgccccacgg ggcctgacag
2041tgagtctgtg cttagcgtca gtcgtcagga agggaagacc aaggacccgt acagcccagt
2101gctcatcctg atgttgccaa ctttcttgta caaagtggtt ggtaagccta tccctaaccc
2161tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc gcgttaagtc
2221gacaatcaac ctctggatta

Download FASTA (ORF) (Full)

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