Construct: ORF TRCN0000479295
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012955.1_s317c1
- Derived from:
- ccsbBroadEn_10033
- DNA Barcode:
- TGCCTTTTGATCCACTCGCCGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEK5 (341676)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479295
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 341676 | NEK5 | NIMA related kinase 5 | NM_199289.3 | 99.9% | 99.8% | 17T>C |
| 2 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_017020557.1 | 90.7% | 90.6% | 1_216del;233T>C |
| 3 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_017020555.1 | 87.5% | 87.4% | 1_216del;233T>C;1785_1786ins75 |
| 4 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_017020556.1 | 81.3% | 79.7% | (many diffs) |
| 5 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_011535066.2 | 79.6% | 79.2% | (many diffs) |
| 6 | human | 341676 | NEK5 | NIMA related kinase 5 | NM_001365552.1 | 78.8% | 77.1% | (many diffs) |
| 7 | human | 341676 | NEK5 | NIMA related kinase 5 | XR_941575.2 | 72.8% | (many diffs) | |
| 8 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_006719808.4 | 65% | 61.8% | (many diffs) |
| 9 | human | 341676 | NEK5 | NIMA related kinase 5 | XR_941574.2 | 63.3% | (many diffs) | |
| 10 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_011535068.2 | 52.3% | 51.7% | (many diffs) |
| 11 | human | 341676 | NEK5 | NIMA related kinase 5 | XR_001749542.1 | 47.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2193
- ORF length:
- 2124
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggataagtac gatgcgatta aggccatcgg gcaaggtgcc ttcgggaaag 121 catacttagc taaagggaaa tcagatagca agcactgtgt cataaaagag atcaattttg 181 aaaagatgcc catacaagaa aaagaagctt caaagaaaga agtgattctt ctggaaaaga 241 tgaaacatcc caacattgta gccttcttca attcatttca agagaatggc aggctgttta 301 ttgtaatgga atattgtgat ggaggggatc tcatgaaaag gatcaataga caacggggtg 361 tgttatttag tgaagatcag atcctcggtt ggtttgtaca gatttctcta ggactaaaac 421 atattcatga caggaagata ttacacaggg acataaaagc tcagaacatt tttcttagca 481 agaacggaat ggtggcaaag cttggggact ttggtatagc aagagtcctg aataattcca 541 tggaacttgc tcgaacttgt attggaacac cttactacct gtccccagag atctgtcaga 601 ataaacccta caacaataaa acggatattt ggtctcttgg ctgtgtctta tatgagctct 661 gcacacttaa acatcctttt gagggtaaca acttacagca gctggttctg aagatttgtc 721 aagcacattt tgccccaata tctccggggt tttctcgtga gctccattcc ttgatatctc 781 agctctttca agtatctcct cgagaccgac catccataaa ttccattttg aaaaggccct 841 ttttagagaa tcttattccc aaatatttga ctcctgaggt cattcaggaa gaattcagtc 901 acatgcttat atgcagagca ggagcgccag cttctcgaca tgctgggaag gtggtccaga 961 agtgtaaaat acaaaaagtg agattccagg gaaagtgccc accaagatca aggatatctg 1021 tgccaattaa aaggaatgct atattgcata gaaatgaatg gagaccacca gctggagccc 1081 agaaggccag atctataaaa atgatagaaa gacccaaaat tgctgctgtc tgtggacatt 1141 atgattatta ttatgctcaa cttgatatgc tgaggaggag agcccacaaa ccaagttatc 1201 accctattcc tcaagaaaat actggagttg aggattacgg tcaggaaacg aggcatggtc 1261 catccccaag tcaatggcct gctgagtacc ttcagagaaa atttgaagct caacaatata 1321 agttgaaagt ggagaagcaa ttgggtcttc gtccatcttc tgccgagcca aattacaacc 1381 agagacaaga gctaagaagt aatggagaag agcctagatt ccaggagctg ccatttagga 1441 aaaacgaaat gaaggaacag gaatattgga agcagttaga ggaaatacgc caacagtacc 1501 acaatgacat gaaagaaatt agaaagaaga tggggagaga accagaggag aactcaaaaa 1561 taagtcataa aacctatttg gtgaagaaga gtaacctgcc tgtccatcaa gatgcatctg 1621 agggagaagc acctgtgcag atggaatttc gctcttgttg cccaggctgg agtgcaatgg 1681 cacgatcttg gctcaccgca acctccgcct cccaggacat tgaaaaagac ttgaaacaaa 1741 tgaggcttca gaacacaaag gaaagtaaaa atccagaaca gaaatataaa gctaagaagg 1801 gggtaaaatt tgaaattaat ttagacaaat gtatttctga tgaaaacaTC CTCCAAGAGG 1861 AAGAGGCAAT GGATATACCA AATGAAACTT TGACCTTTGA GGATGGCATG AAGTTTAAGG 1921 AATATGAATG TGTAAAGGAG CATGGAGATT ATACAGACAA AGCATTTGAA AAACTTCACT 1981 GCCCAGAAGC AGGGTTTTCC ACGCAGACTG TAGCTGCTGT GGGAAACAGG AGGCAGTGGG 2041 ATGGAGGAGC GCCTCAGACT CTGCTGCAGA TGATGGCAGT GGCCGACATC ACCTCCACCT 2101 GCCCCACGGG GCCTGACAGT GAGTCTGTGC TTAGCGTCAG TCGTCAGGAA GGGAAGACCA 2161 AGGACCCGTA CAGCCCAGTG CTCATCCTGA TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2221 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2281 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 2341 CCTTTTGATC CACTCGCCGG ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 2401 ttgtgaaaga tt