Transcript: Human XM_017026748.2

PREDICTED: Homo sapiens zinc finger protein 429 (ZNF429), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF429 (353088)
Length:
5570
CDS:
422..2491

Additional Resources:

NCBI RefSeq record:
XM_017026748.2
NBCI Gene record:
ZNF429 (353088)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017026748.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000242243 TACCCGGTCTTCAAGACTTAC pLKO_005 2356 CDS 100% 0.000 0.000 N ZNF429 n/a
2 TRCN0000242244 GCTATACAAAGGAGGTTATAA pLKO_005 838 CDS 100% 15.000 10.500 N ZNF429 n/a
3 TRCN0000257194 TTCAAGACTTACTCGACATAA pLKO_005 1609 CDS 100% 13.200 9.240 N ZNF429 n/a
4 TRCN0000242245 TAATCGGTCCTCAAGACTTAC pLKO_005 2272 CDS 100% 10.800 7.560 N ZNF429 n/a
5 TRCN0000242246 TTAGCATATCCTCAACCTTTA pLKO_005 1347 CDS 100% 10.800 7.560 N ZNF429 n/a
6 TRCN0000428574 CTGAAGAGAAACCCTACAAAT pLKO_005 1389 CDS 100% 13.200 6.600 Y ZNF138 n/a
7 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 1221 CDS 100% 13.200 6.600 Y Zfp934 n/a
8 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 1221 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
9 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 1221 CDS 100% 13.200 6.600 Y EG668616 n/a
10 TRCN0000018502 GATGTTAGAGAACTACAGAAA pLKO.1 562 CDS 100% 4.950 2.475 Y ZNF493 n/a
11 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2417 CDS 100% 13.200 6.600 Y LIAS n/a
12 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 2916 3UTR 100% 10.800 5.400 Y SMIM11A n/a
13 TRCN0000158848 GAGAAACCTTACAAATGTGAT pLKO.1 2066 CDS 100% 4.950 2.475 Y ZNF28 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017026748.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15167 pDONR223 53.6% 66.5% 28.3% None (many diffs) n/a
2 ccsbBroad304_15167 pLX_304 0% 66.5% 28.3% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_13028 pDONR223 100% 65% 57.2% None (many diffs) n/a
4 ccsbBroad304_13028 pLX_304 0% 65% 57.2% V5 (many diffs) n/a
5 TRCN0000468257 GTACACCAGACCACTACATGCGAC pLX_317 25.6% 65% 57.2% V5 (many diffs) n/a
6 ccsbBroadEn_15729 pDONR223 0% 10.2% 9.3% None (many diffs) n/a
7 ccsbBroad304_15729 pLX_304 0% 10.2% 9.3% V5 (many diffs) n/a
8 TRCN0000470492 GCCGACTTGCTCCATGATGCAGCT pLX_317 100% 10.2% 9.3% V5 (many diffs) n/a
9 ccsbBroadEn_13746 pDONR223 100% 9.9% 9.5% None (many diffs) n/a
10 ccsbBroad304_13746 pLX_304 0% 9.9% 9.5% V5 (many diffs) n/a
11 TRCN0000475669 ATTCGCAGCGAGTTTGTCCGCCGC pLX_317 100% 9.9% 9.5% V5 (many diffs) n/a
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