Transcript: Human XM_024453857.1

PREDICTED: Homo sapiens coiled-coil and C2 domain containing 1B (CC2D1B), transcript variant X9, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CC2D1B (200014)
Length:
4141
CDS:
1817..3448

Additional Resources:

NCBI RefSeq record:
XM_024453857.1
NBCI Gene record:
CC2D1B (200014)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024453857.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000168106 CGGAAAGTCAACTTTGCTGAA pLKO.1 2183 CDS 100% 4.050 3.240 N CC2D1B n/a
2 TRCN0000434529 AGGCACGGAAACTGCAGTATC pLKO_005 2493 CDS 100% 10.800 7.560 N CC2D1B n/a
3 TRCN0000418650 AGTTTGAGATCTTCCACAAAG pLKO_005 3207 CDS 100% 10.800 7.560 N CC2D1B n/a
4 TRCN0000172915 GCTGGAGAATGAGTGTGAGAT pLKO.1 3283 CDS 100% 4.950 3.465 N CC2D1B n/a
5 TRCN0000172971 GCCTCAAGTTCTAAGGAGTCA pLKO.1 2435 CDS 100% 2.640 1.584 N CC2D1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024453857.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14427 pDONR223 100% 96.4% 94.8% None (many diffs) n/a
2 ccsbBroad304_14427 pLX_304 0% 96.4% 94.8% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000471693 GACGGCAGGGCAGTCACCTAAGGA pLX_317 22.7% 96.4% 94.8% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_13380 pDONR223 100% 29% 29% None 1_1155del n/a
5 ccsbBroad304_13380 pLX_304 0% 29% 29% V5 1_1155del n/a
6 TRCN0000467342 ATAAAATCCCCCATTCACTACGTG pLX_317 88.2% 29% 29% V5 1_1155del n/a
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